Acellular matrix fibrous bone composite material and preparation method thereof
A decellularized matrix and composite material technology, applied in the field of decellularized matrix fibrous bone composite material and its preparation, can solve the problems of difficulty in maintaining a specific shape, poor uniformity of pores, and limitation of natural cancellous bone, and achieve convenient and convenient use. Shaping and increasing the effect of local new bone formation
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Embodiment 1
[0036] Such as figure 1 As shown, this embodiment provides a kind of decellularized matrix fiber bone composite material, and its preparation steps include:
[0037] S1-1. Take animal tissues and prepare acellular matrix (including but not limited to: acellular dermal matrix, acellular peritoneal matrix, acellular pericardium matrix, and acellular bladder membrane matrix. The preparation methods will not be repeated here. Those skilled in the art can prepare according to Chinese patent CN103191466B or other similar methods);
[0038] S1-2. Take the wet acellular matrix and cut it into a size less than 1cm 2 After the fragments, carry out high-speed smashing within 1min;
[0039] S1-3. By weight percentage, take 0.5% wet weight of the acellular matrix, 0.01% of the glacial acetic acid and the rest of the purified water, mix and cool down to 2°C;
[0040] S1-4. Homogenize at a frequency of 25 Hz, homogenize the collected material liquid three times, filter out coarse particle...
Embodiment 2
[0053] Such as Figure 2-3 As shown, this embodiment provides another acellular matrix fiber-bone composite material, the preparation steps of which include:
[0054] S1-1, take animal tissue, prepare acellular matrix (same as Example 1);
[0055] S1-2. Take the wet acellular matrix and cut it into a size less than 1cm 2 After the fragments, carry out high-speed mashing within 1min;
[0056] S1-3. By weight percentage, take 3.0% wet weight of the acellular matrix, 0.1% of the glacial acetic acid and the rest of the purified water, mix and cool down to 8°C;
[0057] S1-4. Perform homogenization at a frequency of 29 Hz, and then homogenize the collected feed liquid for 3 times, filter out coarse particles, and use an 8% NaOH solution to adjust the pH of the filtered slurry After reaching 7.5, centrifuge and make an aqueous dispersion to obtain decellularized matrix fibers;
[0058] S2-1, take animal cancellous bone, prepare decellularized cancellous bone (same as embodiment 1)...
Embodiment 3
[0070] Table 1
[0071] Test case comparative example Hydrophilic Hydrophilic Hydrophobic Liquid Absorbency Absorb liquid 2~10 times (porous) does not absorb liquid Performance Easy to use and easy to shape granular, not easily plasticized
[0072] Among them, the test example is the acellular matrix fibrous bone composite material prepared in Example 2; the comparative example is the decellularized cancellous bone in Example 1 of Chinese patent CN103191466B, which is subjected to initial crushing, pretreatment, calcining, re-crushing, and sieving in sequence. The calcined bone particles obtained after separation; the liquid absorption capacity refers to the comparison between the increased weight after absorbing liquid and the original dry state weight.
[0073] In summary, the present invention adopts acellular matrix fibers to simulate the collagen fiber ratio and pores of human bone, and the prepared acellular matrix fibrous bon...
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