Preparation method and application of high-activity lactobacillus fermenti grx08 fermentation liquor
A Lactobacillus fermentum, high activity technology is applied in the field of preparation of the fermentation broth of highly active Lactobacillus fermentum grx08, can solve the problems of unstable lactobacillus content, poor survival rate, etc., achieves food quality assurance, food quality improvement, and economic benefits Effect
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Embodiment 1
[0036] A process for preparing synbiotic coagulated fermented milk using a combination of compound oligosaccharides to increase the number of viable bacteria, the specific steps are as follows:
[0037] Mix papain enzymatically hydrolyzed whey protein hydrolyzate with 0.28% fructo-oligosaccharide, 0.4% galacto-oligosaccharide, 0.4% inulin, 0.8% stachyose, 0.05% xylo-oligosaccharide, filter and homogenize under 20Mpa Finally, heat treatment at 95°C for 5-10 minutes, cool to about 37°C, inoculate with 3% Lactobacillus fermentum grx08 starter, ferment at 37°C for 18 hours, and refrigerate at 4°C for 24 hours to obtain highly active Lactobacillus fermentum grx08 fermentation broth.
Embodiment 2
[0039] Mix papain enzymolyzed whey protein hydrolyzate with 0.30% fructo-oligosaccharide, 0.5% galacto-oligosaccharide, 0.3% inulin, 0.7% stachyose, 0.03% xylo-oligosaccharide, filter and homogenize under 20Mpa Finally, heat treatment at 95°C for 5-10 minutes, cool to about 37°C, inoculate with 3% Lactobacillus fermentum grx08 starter, ferment at 37°C for 18 hours to obtain a fermented liquid, stir the fermented liquid, add sterilized dissolved stabilizer 1. An aqueous solution of an emulsifier, and the aqueous solution is 3 times that of the fermented liquid, homogenized, filled into a sales container, and refrigerated at 4°C for 24 hours to obtain a highly active Lactobacillus fermentum grx08 beverage. The number of live bacteria of Lactobacillus fermentum grx08 in the beverage was 1.23×10 9 CFU / mL.
Embodiment 1、2
[0041] The preparation method of papain enzymolysis whey protein hydrolyzate is as follows:
[0042] In 10.0%~12.0%w / w whey powder, add papain 1000-5000U·g -1 , the enzymolysis temperature is 55~65℃, and the enzymolysis time is 2~3h. After the enzymolysis is completed, it is centrifuged, the supernatant is taken, and the enzyme is inactivated at 105℃ / 5min to obtain the papain enzymolysis whey protein hydrolyzate.
[0043]The preparation method of Lactobacillus fermentum grx08 starter is as follows: the first-generation strain of Lactobacillus fermentum grx08 was inoculated in MRS liquid medium according to the inoculation amount of 2-4%, and placed in a 37°C incubator for 18 hours to obtain;
[0044] Among them, when preparing the MRS liquid medium, 10g of soybean peptone, 10g of beef extract, 5g of yeast extract, 20g of glucose, 801ml of Tween, 2g of sodium dihydrogen phosphate, 5g of anhydrous sodium acetate, 2g of triamine citrate, and 0.02 g of manganese sulfate g. Add 0....
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