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Novosphingobium capsulatum and application thereof

A new technology of Sphingobacterium and strains, applied in the field of microorganisms, can solve the problem of low solubility of insoluble phosphates

Active Publication Date: 2021-12-07
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The cycle of soil phosphorus is mainly centered on microorganisms. Microorganisms that can convert ineffective phosphorus into plants that can absorb and utilize phosphorus are called soil phosphorus conversion microorganisms, also known as phosphorus-dissolving microorganisms, among which bacteria are called phosphorus-dissolving bacteria or phosphorus-solubilizing bacteria. , but traditional phosphate solubilizing bacteria have low solubility to insoluble phosphate

Method used

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  • Novosphingobium capsulatum and application thereof
  • Novosphingobium capsulatum and application thereof
  • Novosphingobium capsulatum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The screening of embodiment 1 phosphate-dissolving bacteria

[0024] Reyan No. 2 Stylosanthes guianensis cv. Reyan No.2 in the vegetative stage was selected, dried and sieved (2mm) as a green manure sample, the experimental base of the Institute of Tropical Crops Variety Resources, Chinese Academy of Tropical Agricultural Sciences, Danzhou City, Hainan Province The brick red soil is the soil sample. The indoor soil column culture method is adopted. One end of the wax cylinder is sealed with two layers of filter paper. The soil to be tested is evenly added to the wax cylinder, and the soil column absorbs water evenly to the saturated water holding capacity by capillary action. Finally, seal the whole wax cylinder with plastic wrap, and place it vertically in the incubator to avoid light and balance for 48 hours. After the soil column is balanced, the phosphate fertilizer or green manure is mixed evenly and placed in a nylon bag, placed on the top of the soil column, seal...

Embodiment 2

[0025] The identification of embodiment 2 phosphate-solubilizing bacteria strains

[0026] Purified and cultivated strains were selected and sequenced by 16S rDNA. The sequences obtained by the sequencing were compared in NCBI, and the sequence with the highest similarity was selected as the species identification result. The similarity between the sequence and the 16S rDNA gene sequence of Novosphingobium capsulatum is as high as 99%.

Embodiment 3

[0027] The ability of embodiment 3 phosphate-solubilizing bacteria to dissolve insoluble phosphate

[0028] Add the same amount of bacterial strain (OD600 is 0.5) into the PKO liquid medium with calcium phosphate, iron phosphate, aluminum phosphate, and phosphate rock powder as the phosphorus source, and place it on a constant temperature shaker (160 r / min , 28°C) for 7 days, centrifuge the culture solution at 4°C (10000r / min) for 15 min, take the supernatant and use the molybdenum-antimony anti-colorimetric method to measure its available phosphorus content, and at the same time, no bacterial suspension was used as a control. The test set was repeated 3 times. The measured available phosphorus content is as follows:

[0029] Available phosphorus content of Novosphingobium capsulatumP3 in four kinds of phosphorus source culture solution (μ g mL -1 )

[0030]

[0031] The formula for calculating the amount of dissolved phosphorus is:

[0032] Calculation formula: P=(K-K ...

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Abstract

The invention discloses Novosphingobium capsulatum and an application thereof. The strain is novosphingobium capsulatum P3, the Novosphingobium capsulatum P3 is preserved in the China Center for Type Culture Collection, the preservation date is May 31, 2021, and the preservation number is CCTCC NO: M2021646. The strain can convert indissolvable inorganic phosphate into soluble phosphorus which can be directly absorbed and utilized by plants, the phosphorus solubilizing quantities of the strain cultured in four indissolvable phosphate liquid culture media including calcium phosphate, iron phosphate, aluminum phosphate and ground phosphate rock for 7 days are 63.1 [mu]g mL <-1>, 4.2 [mu]g mL <-1>, 2.1 [mu]g mL <-1> and 13.3 [mu[g mL <-1> respectively, a certain reference is provided for later research of wild strains, and the Novosphingobium capsulatum has an important value for development of microbial fertilizers such as phosphate-solubilizing bacterial fertilizers and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a new sphingosphingobium capsulatum and an application thereof. Background technique [0002] Phosphorus is one of the most important nutrients needed for plant growth. There are three forms of soil inorganic phosphorus, water-soluble, mineral and adsorbed, of which the mineral form mainly exists. Mineral phosphorus is mainly apatite in alkaline soils and iron aluminum phosphate in acidic soils. Adsorbed phosphorus refers to the phosphate absorbed by the soil solid phase such as mineral or organic matter in the soil, and its content is generally very low. Water-soluble phosphorus can be dissolved in water and can be directly absorbed and utilized by plants. The content in the soil is very low, mainly produced from the dissolution of mineral phosphorus and the release of adsorbed phosphorus. The soil acid-base environment has a direct impact on the form of ph...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/20C12N1/02
Inventor 黄冬芬郇恒福王悠董荣书刘国道
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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