Application of nosiheptide to preparation of periodontitis treatment medicine
A treatment drug, nosiheptide technology, applied in the field of medicine, can solve the problems of inapplicability of oral administration and systemic injection administration, no report on the application of nosiheptide, low bioavailability, etc., to improve BV/TV, The effect of reducing Tb.Sp and promoting inflammation repair
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[0034] 1. Materials and Methods
[0035] 1.1 Drugs and reagents
[0036] Thiostrepton (TSR) (Shanghai Biide Technology Co., Ltd. batch number: ATC114);
[0037] Nosiheptide (NOS) (Shanghai Biide Technology Co., Ltd., batch number: BPW785);
[0038] β-cyclodextrin (Shanghai Beide Technology Co., Ltd. batch number: BGW567);
[0039] Minocycline hydrochloride ointment (Perio, Perio) (Sunstar INC. registration number: H20150106);
[0040] NF-kβELISA kit (USCN KIT INC batch number: SEB824Ra);
[0041] TNF-α kit (Hangzhou Lianke Biotechnology Co., Ltd. batch number: 70-EK981-96);
[0042] Disposable vacuum sampler (Guangzhou Yangpu Medical Technology Co., Ltd.);
[0043] Orthodontic ligature (diameter 0.2mm).
[0044] 1.2 Establishment of experimental rat periodontitis model
[0045]A total of 32 healthy Spraguedawley (SD) male rats, weighing between 200 g and 250 g, were included. Six rats were randomly selected as the normal control group (C0 group), and the remaining 26 r...
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