Bacillus subtilis and application thereof in disease prevention and yield increase of hot peppers
A technology of Bacillus subtilis and pepper, which is applied in the field of agricultural microorganisms, can solve the problems of infecting the growth period of pepper, reducing yield, and cutting off the harvest of pepper, and achieves the effect of being suitable for popularization and application, increasing yield, and significant control effect
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Embodiment 1
[0014] Example 1 Isolation and Identification of GT018-JZ18111
[0015] Soil samples were taken, and purified cultured bacterial strains were obtained through the plate dilution method. Through the verification of pathogenic bacteria related tests, it was found that one of the strains had obvious inhibitory effect on the black stem pathogen of pepper ( figure 1 ):Such as figure 2 As shown, the antagonism between bacteria and bacteria was screened on the plate, and the four Bacillus subtilis strains were respectively labeled as 5, 6, 7, and 8, and No. 6 had the best antagonistic effect, and it was named GT018-JZ18111, referred to as JZ18111 .
[0016] Strain JZ18111 has the following characteristics:
[0017] Cells are rod-shaped, Gram-positive, with a size of 0.6-0.8 μm×2.0-4.0 μm.
[0018] The spores are terminal to subterminal, the cysts are not enlarged, and the spores are cylindrical.
[0019] On the broth medium, the 72h colony was round, flat, moist, white with irre...
Embodiment 2
[0025] Example 2 Preparation of fermentation broth of bacterial strain JZ18111
[0026] The slant preservation medium for strain JZ18111 is beef extract peptone medium: 3g beef extract, 10g peptone, 5g sodium chloride, and 20g agar, put in 1000mL water, pH7.2-7.4, and autoclave at 121°C for 30min after aliquoting.
[0027] Before fermentation, the JZ18111 slant was activated at room temperature for 4-6 hours, and then transferred to the seed liquid medium. The composition of the seed liquid medium is: 3g of beef extract, 10g of peptone, and 5g of sodium chloride, which are placed in 1000mL of sterile water. The seed culture conditions are: 32-35° C., 180-200 rpm / min, and culture for 16-20 hours.
[0028] The cultured seed liquid is added to the fermented liquid according to the mass ratio of 3%-5% to prepare the fermented liquid. The formula of the fermented liquid: per 1000mL of water, add 0.1% of bean cake powder, 0.5% of corn flour and calcium carbonate according to the ma...
Embodiment 3
[0029] Embodiment 3 field test
[0030] The test site is located in the agricultural planting base of Lianchi District, Baoding City, Hebei Province; the terrain is an alluvial plain, flat and evenly fertile, which is at a medium level in the local area, representing an area of 200 mu; soil type: fluvo-aquic soil; soil texture: medium soil; previous stubble Crop: cucumber; fertilization amount of previous crops: compound fertilizer 80kg / mu, organic fertilizer 2000kg / mu; previous crop yield 4100kg / mu; soil nutrient status: pH7.8, organic matter 12.6g / kg, total nitrogen 0.89g / mu kg, available phosphorus 12.5mg / kg, available potassium 116mg / kg.
[0031] The experiment consisted of 4 treatments and 3 repetitions, using a completely randomized block design. Residential area 6.00m×4.00m=24.00m 2 .
[0032] Treatment 1: conventional fertilization;
[0033] Treatment 2: 10% reduction in conventional fertilization + preparation of fermented liquid in Example 2;
[0034] Treatmen...
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