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Metal organic framework multi-enzyme composite catalytic material, preparation method, and application and use method of metal organic framework multi-enzyme composite catalytic material

A technology of metal-organic frameworks and catalytic materials, applied in biochemical equipment and methods, fixed on/in organic carriers, enzymes, etc., can solve the problems of difficult repeated use and difficult collection of enzymes for repeated use , to achieve the effect of improving the recycling rate, mild synthesis conditions and reducing production costs

Pending Publication Date: 2021-12-17
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: The purpose of the present invention is to provide a metal-organic framework multi-enzyme composite catalytic material to solve the problem that enzymes are difficult to collect and reuse repeatedly, while reducing the cost of continuous enzyme catalysis, reducing the impact of the environment on enzyme activity, and difficult to collect multiple reuse

Method used

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  • Metal organic framework multi-enzyme composite catalytic material, preparation method, and application and use method of metal organic framework multi-enzyme composite catalytic material
  • Metal organic framework multi-enzyme composite catalytic material, preparation method, and application and use method of metal organic framework multi-enzyme composite catalytic material
  • Metal organic framework multi-enzyme composite catalytic material, preparation method, and application and use method of metal organic framework multi-enzyme composite catalytic material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] FDH / F ald Preparation of DH / ADH@ZPF:

[0054] First set FDH, F ald DH, ADH (FDH, F aldThe concentrations of DH and ADH are both 0.3mg / mL) into Tris-HCl buffer (pH=7, 0.1M), then add 2-hydroxy-5-fluoropyrimidine (0.2M), and finally add zinc nitrate (0.05M) and stir After uniformity, react at 35°C for 12h. After the reaction, centrifuge at 8000rpm for 4min to recover the precipitate. It was then washed with ultrapure water, sonicated and centrifuged three times to remove free enzyme not encapsulated by the ZPF material.

Embodiment 2

[0056] (FDH / F ald Preparation of DH / ADH@ZPF) / UGDH@MOF:

[0057] 1. (FDH / F ald DH / ADH@ZPF) / UGDH@ZIF-8

[0058] Take the prepared 0.3mg FDH / F ald DH / ADH@ZPFs powder was added to 1mL Tris-HCl buffer solution, stirred evenly, added to 2-methyl-imidazole (HmIm, 640mM) solution, and then added Zn(NO 3 ) 2 ·6H 2 O (40mM) solution and add ultrapure water to make up to 3mL. React at 37° C. and 300 rpm for 24 hours. After the reaction, centrifuge at 8,000 rpm for 4 minutes to recover the precipitate. It was then washed with ultrapure water, sonicated and centrifuged three times.

[0059] 2. (FDH / F ald DH / ADH@ZPF) / UGDH@ZIF-90

[0060] Take the prepared 0.3mg FDH / F ald DH / ADH@ZPFs powder was added to 1mL Tris-HCl buffer solution, stirred evenly, added to 2-imidazole-carboxaldehyde (HICA, 160mM) solution, and then added Zn(NO 3 ) 2 ·6H 2 O (40mM) solution and add ultrapure water to make up to 3mL. React at 37° C. and 200 rpm for 24 hours. After the reaction, centrifuge at 8...

Embodiment 3

[0064] Etching of Template ZPF and FDH / F ald Preparation of DH / ADH / UGDH@MOF:

[0065] Will prepare (FDH / F ald DH / ADH@ZPF) / UGDH@MOF powder was added to PB (pH=5, 50mM) buffer solution and stirred, centrifuged (8000rpm, 5min) after 0.5h, washed, and dried at 35°C to obtain a solid.

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Abstract

The invention discloses a metal organic framework multi-enzyme composite catalytic material, a preparation method, and application and use method of the metal organic framework multi-enzyme composite catalytic material. The composite catalytic material comprises formate dehydrogenase, formaldehyde dehydrogenase, methanol dehydrogenase, UDP-glucose dehydrogenase and an MOF material, and is provided with a hollow capsule cavity; the formate dehydrogenase, the formaldehyde dehydrogenase and the methanol dehydrogenase are wrapped in the capsule cavity, and the UDP-glucose dehydrogenase is immobilized in the MOF material. According to the composite catalytic material of the invention, CO2 can be effectively recycled to provide a potential method for solving the greenhouse effect, repeated utilization can be implemented through immobilization and cyclic regeneration of cofactors while relatively high catalytic activity is kept, and a high-value industrial product is synthesized by a low-cost and environment-friendly method.

Description

technical field [0001] The invention relates to a catalytic material, a preparation method, application and use method, in particular to a metal organic framework multi-enzyme composite catalytic material, a preparation method and an application and use method. Background technique [0002] Glycosaminoglycans are a class of linear polysaccharides widely present on animal cell surfaces and intercellular matrices, and are complex carbohydrate macromolecules. Glycosaminoglycans are also related to many important diseases such as inflammation, tumor, cancer and so on. In addition to being an important functional substance in the body, it is also an important class of biological drugs, such as antithrombotic, treatment of arthritis, etc. The most common glycosyl donor in organisms is UDP-sugar, in which UDP-glucose is the basic raw material for forming other UDP-monosaccharides, and UDP-glucuronic acid is the conversion of UDP-sugar from six-carbon sugar to five-carbon sugar ke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/089C12N11/098C12N11/04C12N9/02C12N9/04C08G83/00C12P7/04C12P19/30
CPCC12N11/089C12N11/098C12N11/04C12N9/0008C12N9/0006C08G83/008C12P7/04C12P19/305C12Y102/01002C12Y102/01046C12Y101/01244C12Y101/01022
Inventor 张幸黄和纪元曹敏沈宝星
Owner NANJING NORMAL UNIVERSITY
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