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Composite microbial agent for degrading oil pollutants in oily sludge and preparation method and application thereof

A technology for compounding microbial inoculants and pollutants, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unsatisfactory effect of oily sludge, differences in oily sludge properties and components, and improve crude oil. Degradation effect, reduction of oil concentration in sludge, effect of good application prospect

Pending Publication Date: 2021-12-31
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, the effect of using a single strain to treat oily sludge is not ideal. The reason is that a single strain has specificity. Due to the differences in crude oil concentration and mining technology in various regions, there are differences in the properties and components of oil sludge. Therefore, single degradation It is difficult for strains to achieve good degradation effects on all kinds of oil sludge

Method used

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  • Composite microbial agent for degrading oil pollutants in oily sludge and preparation method and application thereof
  • Composite microbial agent for degrading oil pollutants in oily sludge and preparation method and application thereof

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preparation example Construction

[0025] The present invention also provides a method for preparing a composite microbial bacterial agent for degrading oily pollutants in oily sludge, comprising the following steps:

[0026] 1) Solid slant culture: Inoculate Pseudomonas aeruginosa, Bifidobacterium, Bacillus subtilis and Mycobacterium salidenitritis respectively in beef extract peptone solid medium, and culture at constant temperature to fully activate the strains;

[0027] 2) Primary seed culture: Inoculate the activated Pseudomonas aeruginosa, Bifidobacterium, Bacillus subtilis and Mycobacterium salidenitritis respectively in the beef extract peptone liquid medium in step 1), and cultivate at constant temperature. OD 600 When the value reaches 0.8, the cultivation is stopped, and the primary seed liquids of Pseudomonas aeruginosa, Bifidobacterium, Bacillus subtilis and Mycobacillus salidenitritis are respectively obtained;

[0028] 3) Secondary seed culture: Inoculate the primary seed liquids of Pseudomonas ...

Embodiment 1

[0034] A compound microbial bacterial agent for degrading oily pollutants in oily sludge, the preparation method of which is as follows:

[0035] 1) Solid slant culture: inoculate Pseudomonas aeruginosa, Domibacillus antri strain XD80, Bacillus subtilis and Mycobacterium salidenitritis in beef extract peptone solid medium respectively, and culture at 37°C for 2 days to fully activate the strains Wherein, the beef extract peptone solid medium formula is: beef extract 0.5wt%, peptone 1.0wt%, sodium chloride 0.5wt%, agar 2wt%, the rest is water, and the pH value is 7.0-7.5;

[0036] 2) Primary seed culture: inoculate Pseudomonas aeruginosa, Bifidobacterium, Bacillus subtilis and Mycobacterium salidenitrophilus activated in step 1) respectively in beef extract peptone liquid medium, at 37°C, Cultured at 130r / min for 2 days, when OD 600 When the value reaches 0.8, the cultivation is stopped, and the first-grade seed liquids of Pseudomonas aeruginosa, Bifidobacterium, Bacillus subt...

Embodiment 2

[0049] Different from Example 1, the composite microbial bacterial agent involved in this embodiment is composed of Pseudomonas aeruginosa bacterial agent, Bifidobacterium bacterial agent, Bacillus subtilis bacterial agent and Mycobacterium salt denitrification bacterial agent by volume It is mixed with a ratio of 1:2:3:4; wherein, the Bifidobacterium used is specifically Bifidobacterium bifidum;

[0050] The fermentation medium used to prepare the composite microbial agent, in terms of mass percentage, includes: 0.3% ammonium chloride, 0.3% potassium dihydrogen phosphate, 0.1% dipotassium hydrogen phosphate, 0.001% anhydrous calcium chloride , 0.3% sodium chloride, 0.01% magnesium sulfate heptahydrate, 1% molasses, the balance is water, and the pH value is 7.0.

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Abstract

The invention discloses a composite microbial agent for degrading oil pollutants in oily sludge and a preparation method and application thereof. The composite microbial agent is prepared by mixing a Pseudomonas aeruginosa agent, a Bifidobacterium agent, a Bacillus subtilis agent and a Virgibacillus halodenitrificans agent according to a volume ratio of (1-4): (1-4) : (1-4) : (1-4). The invention also relates to application of the composite microbial agent for degrading oil pollutants in oily sludge in degrading petroleum substances. The composite microbial agent provided by the invention has a good degradation effect on petroleum hydrocarbon substances in the oily sludge, so that the oil substances in the oily sludge can be removed, the oil concentration in the oily sludge is reduced, and a great promotion effect is achieved on the oily sludge treatment work.

Description

technical field [0001] The invention relates to microbial degradation and treatment of oily pollutants, in particular to a composite microbial bacterial agent for degrading oily pollutants in oily sludge and its preparation method and application. Background technique [0002] Crude oil is a mixture of various simple or complex hydrocarbon compounds, generally composed of saturated hydrocarbons, aromatic hydrocarbons, colloids, asphaltenes, and a small amount of non-hydrocarbon compounds such as nitrogen compounds and sulfur compounds, which are found in various industries in my country. Important applications play a decisive role in my country's economic development. Oily sludge refers to the oil-contaminated mixture formed after crude oil is mixed with soil, water, garbage, etc. during the extraction, refining, and transportation of crude oil, which contains a large number of pathogenic bacteria, parasites (eggs), heavy metals, and a large number of benzene series. Many to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/38C12R1/01C12R1/07C02F101/32
CPCC12N1/20C02F11/02C02F2101/32
Inventor 李成涛王瑞朱超闫宇豪汪泽
Owner SHAANXI UNIV OF SCI & TECH
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