Application of a scutellaria baicalensis flavone active ingredient and preparation thereof in preparation of medicine for preventing and/or treating inflammatory storm
A technology of active ingredient, flavonoids of scutellariae, applied in the field of medicine, can solve the problems of mild adverse reactions of inflammatory storm and lack of effective treatment, and achieve the effect of inhibiting cytokine storm in mice
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[0073] 1. Preparation of phospholipid complexes
[0074] Weigh a certain amount of drug and soybean phospholipid according to the drug-to-lipid ratio (1:3.5, w / w), put it in a rotary steamer, add an appropriate amount of reaction solvent, mix and shake well, and wait until the raw material drug and phospholipid are completely dissolved and the compound solution is clarified. Place it on a rotary evaporator for 15-30 minutes, evaporate the solvent at 40°C, dry it under reduced pressure and vacuum for more than 12 hours, and scrape off the solid to obtain the compound.
[0075] Preparation of baicalein-phospholipid complex: Weigh 1 g of baicalein and 3.5 g of phospholipid S75, put them in a rotary evaporator, add 20 mL of tetrahydrofuran, mix and shake well, wait for the raw material drug and phospholipid to dissolve completely, let it stand for 30 minutes, and place it in a rotary evaporator evaporate the solvent at 40°C, dry under reduced pressure and vacuum for more than 12 h...
Embodiment 1
[0089] Example 1. The active ingredient of Scutellaria baicalensis inhibits the increase of inflammatory cytokines in plasma of mice with systemic inflammatory storm
[0090] (1) Experimental materials
[0091] Baicalein (purity 98%, Nanjing Zelang Biotechnology Co., Ltd., batch number: ZLS1903601);
[0092] Wogonin (National Institute for Food and Drug Control, batch number 111514-201706), Melaleucain A (Chengdu Pufarui Technology Development Co., Ltd., batch number PRF10030442), Lipopolysaccharides from Escherichia coli055:B5 (SIGMA); sodium chloride injection (Sichuan Kelun Pharmaceutical Co., Ltd.); Mouse IL-6 ELISA Kit (Elabscience); Mouse Inflammation Panel, Mouse Cytokine Release Syndrome Panel, Mouse Proinflammatory Chemokine Panel (Biolegend); C57 BL / 6 male mice, 18-22g , (Beijing Huafukang Biotechnology Co., Ltd.); XS 105DU 1 / 100,000 electronic balance (METTLER TOLEDO); 1-14 centrifuge (SIGMA); VORTEX-2 GENIE vortex instrument (Scientific Industries) ; Flowcytomete...
Embodiment 2
[0107] Embodiment 2, the pharmacodynamic experiment of baicalein phospholipid complex self-microemulsion
[0108] The systemic "inflammatory storm" of sepsis was induced by intraperitoneal injection of LPS, and the dosage was investigated. The dosage was as follows: 20mg / kg, 40mg / kg, 80mg / kg, 160mg / kg. The dosage here is the amount of the active ingredient baicalein.
[0109] Experimental method with reference to embodiment 1.
[0110] see results Figure 7 . Depend on Figure 7 It can be seen that 80 mg / kg baicalein self-microemulsion can significantly inhibit the increase of IL-6, but at the doses of 20, 40 and 160 mg / kg, baicalein self-microemulsion has no obvious drug effect, suggesting that Scutellaria baicalensis The efficacy of Suzimicroemulsion does not increase with dose increase. Therefore, this study selected 80mg / kg as the effective dosage of baicalein self-microemulsion, wogonin self-microemulsion and scutellarin A self-microemulsion in vivo efficacy experime...
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