Application of a scutellaria baicalensis flavone active ingredient and preparation thereof in preparation of medicine for preventing and/or treating inflammatory storm

A technology of active ingredient, flavonoids of scutellariae, applied in the field of medicine, can solve the problems of mild adverse reactions of inflammatory storm and lack of effective treatment, and achieve the effect of inhibiting cytokine storm in mice

Pending Publication Date: 2022-01-14
BEIJING WEHAND BIO PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the deficiency in the prior art that there is a lack of drugs that effectively treat inflammatory storms and have mild adverse reactions, the present invention provides the application of the active ingredient of Scutellaria baicalensis in the preparation of drugs for preventing and / or treating inflammatory storms

Method used

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  • Application of a scutellaria baicalensis flavone active ingredient and preparation thereof in preparation of medicine for preventing and/or treating inflammatory storm
  • Application of a scutellaria baicalensis flavone active ingredient and preparation thereof in preparation of medicine for preventing and/or treating inflammatory storm
  • Application of a scutellaria baicalensis flavone active ingredient and preparation thereof in preparation of medicine for preventing and/or treating inflammatory storm

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0073] 1. Preparation of phospholipid complexes

[0074] Weigh a certain amount of drug and soybean phospholipid according to the drug-to-lipid ratio (1:3.5, w / w), put it in a rotary steamer, add an appropriate amount of reaction solvent, mix and shake well, and wait until the raw material drug and phospholipid are completely dissolved and the compound solution is clarified. Place it on a rotary evaporator for 15-30 minutes, evaporate the solvent at 40°C, dry it under reduced pressure and vacuum for more than 12 hours, and scrape off the solid to obtain the compound.

[0075] Preparation of baicalein-phospholipid complex: Weigh 1 g of baicalein and 3.5 g of phospholipid S75, put them in a rotary evaporator, add 20 mL of tetrahydrofuran, mix and shake well, wait for the raw material drug and phospholipid to dissolve completely, let it stand for 30 minutes, and place it in a rotary evaporator evaporate the solvent at 40°C, dry under reduced pressure and vacuum for more than 12 h...

Embodiment 1

[0089] Example 1. The active ingredient of Scutellaria baicalensis inhibits the increase of inflammatory cytokines in plasma of mice with systemic inflammatory storm

[0090] (1) Experimental materials

[0091] Baicalein (purity 98%, Nanjing Zelang Biotechnology Co., Ltd., batch number: ZLS1903601);

[0092] Wogonin (National Institute for Food and Drug Control, batch number 111514-201706), Melaleucain A (Chengdu Pufarui Technology Development Co., Ltd., batch number PRF10030442), Lipopolysaccharides from Escherichia coli055:B5 (SIGMA); sodium chloride injection (Sichuan Kelun Pharmaceutical Co., Ltd.); Mouse IL-6 ELISA Kit (Elabscience); Mouse Inflammation Panel, Mouse Cytokine Release Syndrome Panel, Mouse Proinflammatory Chemokine Panel (Biolegend); C57 BL / 6 male mice, 18-22g , (Beijing Huafukang Biotechnology Co., Ltd.); XS 105DU 1 / 100,000 electronic balance (METTLER TOLEDO); 1-14 centrifuge (SIGMA); VORTEX-2 GENIE vortex instrument (Scientific Industries) ; Flowcytomete...

Embodiment 2

[0107] Embodiment 2, the pharmacodynamic experiment of baicalein phospholipid complex self-microemulsion

[0108] The systemic "inflammatory storm" of sepsis was induced by intraperitoneal injection of LPS, and the dosage was investigated. The dosage was as follows: 20mg / kg, 40mg / kg, 80mg / kg, 160mg / kg. The dosage here is the amount of the active ingredient baicalein.

[0109] Experimental method with reference to embodiment 1.

[0110] see results Figure 7 . Depend on Figure 7 It can be seen that 80 mg / kg baicalein self-microemulsion can significantly inhibit the increase of IL-6, but at the doses of 20, 40 and 160 mg / kg, baicalein self-microemulsion has no obvious drug effect, suggesting that Scutellaria baicalensis The efficacy of Suzimicroemulsion does not increase with dose increase. Therefore, this study selected 80mg / kg as the effective dosage of baicalein self-microemulsion, wogonin self-microemulsion and scutellarin A self-microemulsion in vivo efficacy experime...

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Abstract

The invention discloses application of a scutellaria baicalensis flavone active ingredient and a preparation thereof in preparation of a medicine for preventing and/or treating inflammatory storm. The active ingredient of the scutellaria baicalensis flavone is selected from at least one of the following components: baicalein, wogonin and oroxylin A. Inflammation storm is an excessive immune response and an inflammatory response of an organism to external stimulation, and is characterized by rapid and massive release of inflammatory cytokines. Inflammation storm can be caused by many infectious or non-infectious diseases and is closely related to the severity of the diseases and the occurrence of multi-organ dysfunction syndrome. Reducing the occurrence of inflammatory storm is helpful for reducing organ injury and slowing down disease progress, and is especially crucial for treatment of critical patients. It is found that baicalein, wogonin and oroxylin A all have the effects of inhibiting mouse cytokine storm to different degrees. Baicalein can improve lung injury and inflammatory cell infiltration caused by inflammatory storm. Therefore, the flavonoid active ingredient of scutellaria baicalensis can be used for preparing the medicine for preventing and treating the inflammatory storm.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of active components of baicalin flavonoids and preparations thereof in the preparation of medicines for preventing and / or treating inflammatory storms. Background technique [0002] Inflammatory storm is an excessive immune response and inflammatory response of the body to external stimuli, characterized by the excessive proliferation of T cells and macrophages and the rapid release and overexpression of inflammatory cytokines. Inflammatory storms can be caused by many infectious or non-infectious diseases, such as influenza, new coronary pneumonia (COVID-19), Middle East respiratory syndrome (MERS-CoV), atypical pneumonia (SARS), familial hemophagocytic syndrome, sepsis, systemic Juvenile idiopathic arthritis (sJIA), chimeric antigen receptor (CAR)-T cell therapy and lupus erythematosus, etc. [0003] The inflammatory storm phenomenon caused by respiratory v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K36/539A61K9/107A61K47/24A61P29/00
CPCA61K31/352A61K36/539A61K9/1075A61K47/24A61P29/00
Inventor 刘玉玲廖恒锋叶军刘志华高越杨艳芳廉春放刘璐徐晓燕冯遇姜丹刘瑾如王丽
Owner BEIJING WEHAND BIO PHARMA CO LTD
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