Anti-degradation gelatin microspheres, artificial liver model and construction method and application of artificial liver model
An anti-degradation, microsphere technology, applied in the field of biomedical materials and biomedical engineering, can solve the problem that it cannot meet the needs of mass production and industrialization, cannot accurately simulate the complex structure and function of liver tissue, and cannot comprehensively and accurately reflect liver damage. process and other issues, to achieve the effect of being beneficial to liver function expression and long-term in vitro application, improving anti-degradation properties, and excellent cytocompatibility
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Embodiment 1
[0044] The preparation steps of the anti-degradation gelatin microspheres provided in this example are as follows:
[0045] (1) Synthesis of methacrylamide gelatin
[0046] Under stirring conditions, weigh 20g of gelatin powder and dissolve it in 100mL of PBS buffer solution at 50°C to form a gelatin solution with a mass concentration of 200mg / mL; then, add 5mL of methacrylic anhydride dropwise to the above gelatin solution; the formed The reaction system was reacted at 60°C in the dark for 5 hours; during the reaction process, 2mol / L NaOH solution was continuously used to adjust the pH value of the reaction system to 8; after the reaction was completed, an equal volume of absolute ethanol was added to precipitate the precipitate, and then the supernatant was removed by centrifugation solution, and washed three times by centrifugation with absolute ethanol. Dissolve the final centrifuged product in 100 mL of deionized water, and use deionized water to avoid light for 7 days a...
Embodiment 2
[0052] The preparation steps of the anti-degradation gelatin microspheres provided in this example are as follows:
[0053] (1) Synthesis of methacrylamide gelatin
[0054] Under stirring conditions, weigh 10g of gelatin powder and dissolve it in 100mL of PBS buffer solution at 60°C to form a gelatin solution with a mass concentration of 100mg / mL; then, add 5mL of methacrylic anhydride dropwise to the above gelatin solution; the formed The reaction system was reacted at 60°C in the dark for 3 hours; during the reaction process, 5mol / L NaOH solution was continuously used to adjust the pH value of the reaction system to 8; after the reaction was completed, an equal volume of absolute ethanol was added to precipitate the precipitate, and then the supernatant was removed by centrifugation solution, and washed three times by centrifugation with absolute ethanol. Dissolve the final centrifuged product in 100 mL of deionized water, and use deionized water to avoid light for 5 days a...
Embodiment 3
[0060] The preparation steps of the anti-degradation gelatin microspheres provided in this example are as follows:
[0061] (1) Synthesis of methacrylamide gelatin
[0062] Under stirring conditions, weigh 10g of gelatin powder and dissolve it in 100mL of PBS buffer at 60°C to form a gelatin solution with a mass concentration of 100mg / mL; then, add 10mL of methacrylic anhydride dropwise to the above gelatin solution; the formed The reaction system was reacted at 60°C in the dark for 3 hours; during the reaction process, 5mol / L NaOH solution was continuously used to adjust the pH value of the reaction system to 8; after the reaction was completed, an equal volume of absolute ethanol was added to precipitate the precipitate, and then the supernatant was removed by centrifugation solution, and washed three times by centrifugation with absolute ethanol. Dissolve the final centrifuged product in 50 mL of deionized water, and use deionized water to avoid light for 3 days at 4°C to ...
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