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Anti-degradation gelatin microspheres, artificial liver model and construction method and application of artificial liver model

An anti-degradation, microsphere technology, applied in the field of biomedical materials and biomedical engineering, can solve the problem that it cannot meet the needs of mass production and industrialization, cannot accurately simulate the complex structure and function of liver tissue, and cannot comprehensively and accurately reflect liver damage. process and other issues, to achieve the effect of being beneficial to liver function expression and long-term in vitro application, improving anti-degradation properties, and excellent cytocompatibility

Pending Publication Date: 2022-01-14
LUZHOU GUOZHIRONGYAO LIQUOR IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strong regenerative ability and compensatory function of the liver, these models cannot truly, quickly and sensitively reflect liver lesions or abnormal liver function in the short term, and are costly, difficult to operate, and highly professional, which cannot meet the needs of mass production and industrialization
However, simple in vitro cell models cannot accurately simulate the complex structure and function of liver tissue, and cannot fully and accurately reflect the process of liver injury.

Method used

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  • Anti-degradation gelatin microspheres, artificial liver model and construction method and application of artificial liver model
  • Anti-degradation gelatin microspheres, artificial liver model and construction method and application of artificial liver model
  • Anti-degradation gelatin microspheres, artificial liver model and construction method and application of artificial liver model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation steps of the anti-degradation gelatin microspheres provided in this example are as follows:

[0045] (1) Synthesis of methacrylamide gelatin

[0046] Under stirring conditions, weigh 20g of gelatin powder and dissolve it in 100mL of PBS buffer solution at 50°C to form a gelatin solution with a mass concentration of 200mg / mL; then, add 5mL of methacrylic anhydride dropwise to the above gelatin solution; the formed The reaction system was reacted at 60°C in the dark for 5 hours; during the reaction process, 2mol / L NaOH solution was continuously used to adjust the pH value of the reaction system to 8; after the reaction was completed, an equal volume of absolute ethanol was added to precipitate the precipitate, and then the supernatant was removed by centrifugation solution, and washed three times by centrifugation with absolute ethanol. Dissolve the final centrifuged product in 100 mL of deionized water, and use deionized water to avoid light for 7 days a...

Embodiment 2

[0052] The preparation steps of the anti-degradation gelatin microspheres provided in this example are as follows:

[0053] (1) Synthesis of methacrylamide gelatin

[0054] Under stirring conditions, weigh 10g of gelatin powder and dissolve it in 100mL of PBS buffer solution at 60°C to form a gelatin solution with a mass concentration of 100mg / mL; then, add 5mL of methacrylic anhydride dropwise to the above gelatin solution; the formed The reaction system was reacted at 60°C in the dark for 3 hours; during the reaction process, 5mol / L NaOH solution was continuously used to adjust the pH value of the reaction system to 8; after the reaction was completed, an equal volume of absolute ethanol was added to precipitate the precipitate, and then the supernatant was removed by centrifugation solution, and washed three times by centrifugation with absolute ethanol. Dissolve the final centrifuged product in 100 mL of deionized water, and use deionized water to avoid light for 5 days a...

Embodiment 3

[0060] The preparation steps of the anti-degradation gelatin microspheres provided in this example are as follows:

[0061] (1) Synthesis of methacrylamide gelatin

[0062] Under stirring conditions, weigh 10g of gelatin powder and dissolve it in 100mL of PBS buffer at 60°C to form a gelatin solution with a mass concentration of 100mg / mL; then, add 10mL of methacrylic anhydride dropwise to the above gelatin solution; the formed The reaction system was reacted at 60°C in the dark for 3 hours; during the reaction process, 5mol / L NaOH solution was continuously used to adjust the pH value of the reaction system to 8; after the reaction was completed, an equal volume of absolute ethanol was added to precipitate the precipitate, and then the supernatant was removed by centrifugation solution, and washed three times by centrifugation with absolute ethanol. Dissolve the final centrifuged product in 50 mL of deionized water, and use deionized water to avoid light for 3 days at 4°C to ...

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Abstract

The invention discloses anti-degradation gelatin microspheres, an artificial liver model and a construction method and application of the artificial liver model. Methacrylamide modified gelatin is firstly synthesized, and then photo-crosslinking gelatin microspheres are further prepared through emulsification, so that the gelatin microspheres have the anti-degradation property in a physiological environment; and moreover, simple and efficient regulation and control of the degradation rate of the microspheres can be realized by adjusting the methacrylamide amidation degree and the illumination condition, and a cell carrier is provided for construction of an in-vitro tissue engineering artificial liver. The anti-degradation gelatin microspheres are used as cell carriers, vascular endothelial cells and hepatocyte electrodes are inoculated on the surfaces of the anti-degradation gelatin microspheres, and the artificial liver model is formed through spontaneous bonding and assembling, so that the anti-degradation characteristic of the artificial liver model is improved, the obtained artificial liver model is high in vascularization degree, liver function expression and long-term in-vitro application of the artificial liver model are facilitated, and the artificial liver model can be used for Baijiu quality identification and alcoholic liver disease treatment drug treatment effect evaluation.

Description

technical field [0001] The invention belongs to the technical fields of biomedical materials and biomedical engineering, and relates to a method for manufacturing a bionic material, in particular to a method for constructing a tissue-engineered artificial liver model for rapid evaluation of liquor safety. Background technique [0002] Studies have shown that long-term heavy drinking may cause alcoholic liver disease, and there are differences in liver damage caused by different quality liquors. The degree of liver function damage and morphological changes caused by liquor is related to the type and dose of liquor. Excessive drinking and drinking of low-quality liquor will lead to the accumulation of acetaldehyde, an intermediate metabolite of ethanol, in the body, destroy the cellular structure of liver cells, trigger autoimmune reactions, directly or indirectly lead to liver cell degeneration, necrosis and fibrosis, and also cause Changes in nonparenchymal cells of the live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/28C08L89/00C08H1/00C12N5/071C12Q1/02
CPCC08J3/28C08H1/00C12N5/0671C12N5/0691G01N33/5067C08J2389/00C12N2533/54C12N2503/04C12N2503/02G01N2500/10
Inventor 范宏筠张煜亮税梁扬周帅杨明永郭臻珍
Owner LUZHOU GUOZHIRONGYAO LIQUOR IND CO LTD
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