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Termite-derived biocontrol streptomyces W7 and application thereof

A technology for biological control of Streptomyces and termites, applied in the field of microorganisms, can solve problems such as ecological environment damage, human health impact, and enhance crop drug resistance, and achieve the effects of environmental friendliness, good development prospects, and high biological control potential.

Active Publication Date: 2022-01-14
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the widespread use of chemical pesticides is extremely harmful to the ecological environment, and will also increase crop resistance, and chemical pesticide residues also have an impact on human health

Method used

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  • Termite-derived biocontrol streptomyces W7 and application thereof
  • Termite-derived biocontrol streptomyces W7 and application thereof
  • Termite-derived biocontrol streptomyces W7 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 strain screening

[0038] The termite samples were collected from the park of the Chinese Academy of Tropical Agricultural Sciences in Haikou City, Hainan Province, China. Gaoshi Synthetic No. 1 medium was selected, and the multi-concentration gradient dilution coating method was used to isolate and screen. Observe and pick the strains that conform to the colony form of actinomycetes every day. Transfer to ISP2 medium, separate and purify, and store in glycerol tubes at -20°C.

[0039] The Gaoshi Synthetic No. 1 medium is prepared according to the following components according to the mass volume ratio: soluble starch 20g / L, NaCl 0.5g / L, FeSO 4 0.01g / L, KNO 3 1g / L, K 2 HPO 4 0.5g / L, MgSO4 0.5 g / L, agar 15g / L, final pH 7.3±0.2.

[0040] The ISP2 medium is prepared according to the following components according to the mass volume ratio: yeast extract powder 4g / L, malt extract powder 10g / L, glucose 4g / L, agar 20g / L, pH7.2±0.2.

Embodiment 2

[0041] Embodiment 2 Actinomycetes anti-phytopathogen active assay

[0042] All the isolated actinomycete strains were transferred to PDA medium for cultivation. After each strain produced a sufficient amount of spores, mango anthracnose bacteria were used as indicator bacteria, and the antibacterial activity of actinomycetes was determined by plate confrontation method: prepare a 7mm diameter The mango anthracnose bacteria cake was inoculated in the center of the PDA plate (diameter 90 mm), and the place 2 cm above and below the bacteria cake was the actinomycetes band cultured for 3 days. The mango anthrax bacteria plate that was not inoculated with actinomycetes was used as the control group, and cultured at a constant temperature of 28°C until The control group was measured after covering the whole plate, and each treatment was repeated 3 times. The diameter of the colony was measured by the cross method, and the antibacterial rate of the strain on the mango anthracnose bac...

Embodiment 3

[0046] Molecular Identification of Example 3 Strain W7

[0047] Actinomycete W7 was inoculated onto ISP2 solid plate, cultured at 28°C for 5 days, and a single colony was picked to ISP2 for secondary purification.

[0048] Total DNA was extracted using TSINGKE DNA Extraction Kit (General Type). Universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3') were used for 16S rRNA amplification.

[0049] The total volume of the PCR amplification reaction system is 50 μL, including 45 μL of 1× gold mix DNA polymerase, 2 μL of primer 27F, 2 μL of primer 1492R, and 1 μL of DNA template.

[0050] The amplification conditions were pre-denaturation at 98°C for 2min, denaturation at 98°C for 10s, annealing at 56°C for 10s, extension at 72°C for 10s, and 35 cycles; final extension at 72°C for 5min.

[0051] 0.8% agarose gel was used to detect the fragments obtained after the PCR reaction (voltage 300V, time 12min), and the gel imager was used to observe the e...

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Abstract

The invention discloses a termite-derived biocontrol streptomyces W7, the preservation number is GDMCC No: 61902, the preservation date is August 30, 2021, and the preservation unit is Guangdong Microbial Culture Collection Center. The invention also discloses application of the termite-derived biocontrol streptomyces W7 and the biocontrol product thereof in prevention and treatment of phytopathogen. The obtained biocontrol streptomyces W7 is good in antibacterial effect, stable in prevention and treatment effect, wide in antibacterial spectrum, good in development prospect and large in biological prevention and treatment potential, and for example, antibacterial active components in fermentation extract of the biocontrol streptomyces W7 can be further used as biopesticide to be developed and applied to prevention and treatment of various plant diseases through separation, purification and other technologies. Compared with chemical pesticides, the biocontrol streptomyces W7 and biocontrol products thereof, such as fermentation extracts, have the advantages of no residue, low toxicity, environment friendliness and the like, and are more in line with the current green and environment-friendly concept.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a strain of termite-derived biocontrol Streptomyces W7 and its application. Background technique [0002] Termites, belonging to the Termite family (Termitidae) of the order Blattaria (Blattaria), also known as insect ants, giant water ants, etc., are social insects and omnivorous pests that occur worldwide. Termites live widely in tropical and subtropical regions, and are also found in temperate regions. my country is mainly distributed in the southern region, of which a large number are distributed in Yunnan, Guangdong, and Hainan, and only a few are distributed in North China and Northeast China. Termites are not only widely distributed geographically, but also have a wide range of damage. According to reports, termites can cause harm to important materials such as house construction, garden trees, water conservancy projects, transportation facilities, and telecommunication e...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/14A01N63/28A01P3/00C12R1/465
CPCC12N1/20A01N63/14A01N63/28
Inventor 郭志凯吴炜城张世清陈必婷齐敏于伟伟李依霖
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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