Method for multi-color colorimetric detection of hemoglobin based on gold nanorod etching
A technology of gold nanorods and hemoglobin, which is applied in the direction of measuring devices, material analysis by observing the influence of chemical indicators, instruments, etc., can solve the problems of high requirements for instruments and professional operation, complicated operation of hemoglobin, etc., and achieve high sensitivity , wide linear range and low detection limit
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Embodiment 1
[0026] A method for multicolor colorimetric detection of hemoglobin based on gold nanorod etching, comprising the following steps:
[0027] (1) Add 0.25mL 0.01mol / L chloroauric acid (HAuCl 4 4H 2 O) Add to 9.75mL 0.1mol / L cetyltrimethylammonium bromide (CTAB) and keep stirring rapidly. During the stirring process, quickly add 0.6mL 0.01mol / L NaBH 4 After stirring for 4 minutes, the solution was brown, and the resulting solution was left to stand at 28°C for 3 hours to obtain a gold nano-seed solution; 2 mL of 0.01 mol / L chloroauric acid was added to 40 mL of 0.1 mol / L CTAB and stirred evenly, and then Sequentially add 0.4mL 0.01mol / L AgNO 3 and 0.1mol / L 0.32mL ascorbic acid (AA) and stir evenly, then add 10μL gold nanometer seed solution, shake well and place it at 28°C for about 12h to obtain an aqueous solution of gold nanorods; take 50mL gold nanorods Centrifuge the rod aqueous solution, add 20mL 0.14M CTAB to the centrifuged precipitate to obtain a gold nanorod dispersi...
Embodiment 2
[0033] In order to increase the reaction sensitivity, the experimental conditions of the method for multicolor colorimetric detection of hemoglobin based on gold nanorod etching described in Example 1 were optimized by the single variable method.
[0034] (1) pH of the reaction system: the experiment was carried out in the range of pH 2~7, the results are as follows image 3 As shown in a, the relative catalytic activity of hemoglobin is the highest when the reaction system pH is 5.0, so pH=5.0 is selected as the optimal reaction pH value.
[0035] (2) Concentration of hydrogen peroxide solution: in H 2 o 2 The solution concentration is 0.01 ~ 3M in the range of experiments, the results are as follows image 3 As shown in b, similar to the catalytic activity of horseradish peroxidase (HRP) enzyme, excess H 2 o 2 will inhibit the catalytic activity of the enzyme, with H 2 o 2 As the concentration of the solution increases, the catalytic activity of hemoglobin reaches the ...
Embodiment 3
[0041] The method described in Example 1 was used for visual colorimetric detection of hemoglobin samples with different concentrations. The result is as Figure 5 As shown in a, the results show that with the increase of hemoglobin concentration, the longitudinal absorption peak of gold nanorods near 700nm shows a blue shift, and its absorbance gradually decreases. linear correlation ( Figure 5 b), its linear equation is: Δλ=1.374C (μg / mL)+18.575 (R 2 =0.943), its detection limit is 1.23μg / mL, and different colors appear with different hemoglobin concentrations ( Figure 5 c). Therefore, after etching with different concentrations of hemoglobin, the color change of the gold nanorod solution can be used to effectively measure the concentration of hemoglobin under the naked eye with high sensitivity.
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