Method for immobilizing laccase by ionic liquid modified composite material

A technology for immobilizing laccase and composite materials, which is applied to the field of ionic liquid-modified composite materials immobilizing laccase, can solve the problems of lack of modification of matrix materials, the amount of enzyme immobilization, and the degradation and adsorption effect of the immobilized enzyme is not particularly ideal. , to facilitate modification and modification, promote ion exchange, and improve the internal pore structure.

Active Publication Date: 2022-01-28
JIANGSU YINONG BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above methods, ionic liquids are simple modifications to commonly used nanomaterials (such as carbon nanotubes) and porous materials (such as mesoporous molecular sieves), etc., and the matrix materials used lack necessary modifications, which makes the amount and immobilization of enzymes difficult. The degradation and adsorption effect of enzymes is not particularly ideal

Method used

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  • Method for immobilizing laccase by ionic liquid modified composite material

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Effect test

Embodiment 1

[0030] A method for immobilizing laccase in a composite material modified by ionic liquid, comprising the steps of:

[0031] (1) Preparation of laccase immobilization carrier;

[0032] (2) Immobilization of laccase: at room temperature, take 1.5 mL of laccase-containing acetic acid-sodium acetate buffer solution with a pH of 5.5, then add 0.5 g of laccase-immobilized carrier, shake at room temperature for 2 hours, suction filter and wash Immobilized laccase was obtained; the concentration of laccase was 0.5 g / L.

[0033] Wherein, the preparation of laccase-immobilized carrier comprises the following steps:

[0034] S1. Preparation of composite material: Mix 12g of rectorite after impurity removal and drying with 100mL of deionized water, then add 100g of 0.08g / g ferric sulfate solution and adjust the pH to neutral; then stir and react at room temperature for 1.5h After the reaction is completed, perform suction filtration, washing, and drying treatment, and after grinding, p...

Embodiment 2

[0039] A method for immobilizing laccase in a composite material modified by ionic liquid, comprising the steps of:

[0040] (1) Preparation of laccase immobilization carrier;

[0041] (2) Immobilization of laccase: at room temperature, take 1.5 mL of laccase-containing acetic acid-sodium acetate buffer solution with a pH of 5.5, then add 0.5 g of laccase-immobilized carrier, shake at room temperature for 2 hours, suction filter and wash Immobilized laccase was obtained; the concentration of laccase was 0.5 g / L.

[0042] Wherein, the preparation of laccase-immobilized carrier comprises the following steps:

[0043] S1. Preparation of composite material: Mix 12 g of rectorite after impurity removal and drying with 100 mL of deionized water, then add 100 g of 0.09 g / g ferric sulfate solution and adjust the pH to neutral; then stir and react at room temperature for 2 h, After the reaction is completed, perform suction filtration, washing, and drying treatment, and after grindin...

Embodiment 3

[0048]A method for immobilizing laccase in a composite material modified by ionic liquid, comprising the steps of:

[0049] (1) Preparation of laccase immobilization carrier;

[0050] (2) Immobilization of laccase: at room temperature, take 1.5 mL of laccase-containing acetic acid-sodium acetate buffer solution with a pH of 5.5, then add 0.5 g of laccase-immobilized carrier, shake at room temperature for 2 hours, suction filter and wash Immobilized laccase was obtained; the concentration of laccase was 0.5 g / L.

[0051] Wherein, the preparation of laccase-immobilized carrier comprises the following steps:

[0052] S1. Preparation of composite materials: Mix 12g of rectorite after impurity removal and drying with 100mL of deionized water, then add 100g of 0.095g / g ferric sulfate solution and adjust the pH to neutral; then stir and react at room temperature for 2.5h After the reaction is completed, perform suction filtration, washing, and drying treatment, and after grinding, ...

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Abstract

The invention discloses a method for immobilizing laccase by an ionic liquid modified composite material, the ionic liquid modified composite material is used for immobilization of laccase, and the preparation comprises three main steps of preparation of the composite material, acidification of the composite material and modification of the ionic liquid. In the preparation process, firstly, rectorite is used as a raw material, and the rectorite composite material is prepared through calcination; then, abundant hydroxyl and amino are formed on the surface of the rectorite composite material through acidification and coupling agent modification, and finally, the hydroxyl and the amino are combined with the ionic liquid, so that the carrier is further modified by the ionic liquid. The immobilization amount of the obtained immobilized enzyme is close to 120mg/g; the immobilized enzyme has good degradation and adsorption effects on common phenolic substances such as 2, 4-dichlorophenol, catechol and resorcinol, the degradation and adsorption rate of the resorcinol and the 2, 4-dichlorophenol exceeds 90%, the degradation and adsorption rate of the catechol is close to 80%, and wide application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of laccase immobilization, and in particular relates to a method for immobilizing laccase on a composite material modified by an ionic liquid. Background technique [0002] Laccase is a copper-containing glycoprotein. Due to its special catalytic function and wide range of substrate properties, it is widely used in biology, environmental science, food engineering, chemistry and other technical fields by researchers. The lack of low temperature stability and high cost of laccase greatly restricts the use of laccase alone. Immobilization of laccase can greatly improve the enzyme's anti-solvent, heat resistance and other properties, and improve catalytic activity. [0003] In the immobilization of laccase, the use of carrier is very important. Generally speaking, large specific surface area, porous, and abundant functional groups are the factors that must be considered when selecting immobilized laccase carrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N11/04C12N11/08C12N11/14
CPCC12N9/0061C12N11/04C12N11/14C12N11/08Y02P20/54
Inventor 王俊峰潘建泽张慧君张鹏鹏
Owner JIANGSU YINONG BIOLOGY CO LTD
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