Method of producing hydrogen by culturing marine photosynthetic bacteria for coupled photo and dark fermentation

Abstract

The invention provides a method of producing hydrogen by culturing marine photosynthetic bacteria for coupled photo and dark fermentation, and relates to the field of biological hydrogen production. The method comprises the following steps: conducting culturing with marine bottom mud, seawater and an inorganic substance as a dark fermentation culture medium to obtain a flora, and carrying out enriching to obtain a pure strain of dark zymophyte; with marine bottom mud, seawater and a light fermentation culture medium as substrates, carrying out culturing under an illumination condition to obtain a flora, and conducting enriching to obtain a pure strain of photo zymophyte; and adding tapioca flour into the dark fermentation culture medium, inoculating the dark zymophyte for fermentation so as to produce hydrogen, and inoculating the photo zymophyte to continuously produce hydrogen in 50-60 hours after inoculation of the dark zymophyte. According to the invention, continuous, efficient and stable hydrogen production capacity is realized, a living environment for synergistic growth is created, and two bacteria fully play a symbiotic synergistic complementary role.

Description

technical field [0001] The invention relates to the field of biological hydrogen production, in particular to a method for cultivating marine photosynthetic bacteria and coupling hydrogen production with light-dark fermentation. Background technique [0002] Energy shortage and environmental pollution are two major problems facing human economic and social development, and the development of renewable green energy is imminent. Therefore, we must look for clean renewable energy to replace "fossil energy". Due to its high energy density, convenient transportation, and no pollution, hydrogen energy is most likely to become one of the main alternative energy sources in the future. However, hydrogen in nature exists in the form of a compound state, and hydrogen must be obtained through chemical physical methods or biological methods, so it is also called a secondary energy source. The production of hydrogen by chemical and physical methods consumes fossil energy in addition to ...

AI Technical Summary

Problems solved by technology

Among them, dark fermentation hydrogen production has the advantages of fast hydrogen production speed and simple process conditions, but when the dark fermentation bacteria release hydrogen, with the degradation of organic matter, the pH value continues to drop, and the drop in pH will reduce the hydrogen release activity of the fermentation bacteria. Even completely lose the hydrogen desorption activity, the actual hydrogen production is lower than the theoretical value

Hydrogen production by photofermentative bacteria is a biohydrogen production technology in which photosynthetic bacteria, mainly purple non-sulfur bacteria, can convert various low-molecular organic substrates into hydrogen and carbon dioxide under light anaerobic conditions, but photofermentative bacteria decompose macromolecules The ability of organic matter is not strong, and even organic waste cannot be treated alone to produce hydrogen

Therefore, the current two biohydrogen production methods have relatively large defects.

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