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Burkholderia gladioli P8 and application thereof

A technology of Burkholderia populatum and Holderella, which is applied in the field of microorganisms, can solve the problems of the degradation of phosphorus-solubilizing microorganisms' phosphorus-solubilizing ability, the accumulation and disappearance of phosphorus in crop yields, and the promotion of photosynthesis. Resolving the effect of stunted root development

Active Publication Date: 2022-02-08
GUANGXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although most studies have shown that the phosphorus-dissolving microorganisms screened have strong phosphorus-dissolving ability, many phosphorus-dissolving microorganisms have no stable effect when applied to the soil, and sometimes do not increase crop yield and phosphorus accumulation.
The strain has good phosphorus-dissolving ability in the laboratory, but the phosphorus-dissolving ability is weakened or completely disappeared after it is applied to the soil. The main reasons are as follows: (1) The soil has a complex environment, and phosphorus-dissolving microorganisms can (2) competition between phosphorus-dissolving microorganisms and soil microorganisms; (3) ability of phosphorus-dissolving microorganisms to utilize nutrients in soil; (4) phosphorus-dissolving ability of phosphorus-dissolving microorganisms (5) Soil texture, pH, water content and organic matter content will all affect the growth and reproduction of phosphorus-solubilizing microorganisms and their colonization effect

Method used

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  • Burkholderia gladioli P8 and application thereof
  • Burkholderia gladioli P8 and application thereof
  • Burkholderia gladioli P8 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Isolation, Screening and Identification of Rhizosphere Phosphorus Solubilizing Bacteria of Wangtianshu

[0043] 1.1 Isolation of Phosphorus Solubilizing Bacteria

[0044] In August 2019, at the Arboretum in Nanning, Guangxi, we selected Wangtianshu plantations planted in 1978, 2011, 2012, 2017, 2018, and 2019 to collect soil samples from the root system of Wangtianshu and adhered to the rhizosphere.

[0045]Using lecithin organic phosphorus medium and calcium phosphate inorganic phosphorus medium to isolate phosphorus-dissolving bacteria from the rhizosphere soil of Wangtian tree in different afforestation years, a total of 68 isolates were obtained, of which 30 isolates were in organic phosphorus or inorganic phosphorus Obvious transparent circles can be formed on the solid medium, indicating that these 30 strains have the ability to dissolve insoluble organic phosphorus or inorganic phosphorus, and the 30 strains that can produce phosphorus-dissolving circle...

Embodiment 2

[0078] Example 2 Screening of Phosphorus Solubilizing Bacteria Optimal Phosphorus Solubilizing Conditions in Rhizosphere of Wangtianshu

[0079] The organic phosphorus-solubilizing bacteria P1, P5, P7 and P8, and the inorganic phosphorus-solubilizing bacteria P4, P8, P12 and P30 screened out through the test in Example 1 were used as test materials to further study the phosphorus-solubilizing conditions of the phosphorus-solubilizing bacteria.

[0080] 2.1 The effect of pH value on the phosphorus solubilizing ability of phosphorus solubilizing bacteria

[0081] Prepare organic phosphorus and inorganic phosphorus liquid culture media, respectively, and set the initial pH to 3.5, 4.5, 5.5, 6.5, 7.5, and 8.5, respectively, and distribute them in 150mL Erlenmeyer flasks, 30mL per bottle, and sterilize at 121°C for 20min. 1 mL of organophosphate-decomposing bacterial seed solution was inoculated in organic phosphorus liquid medium with different pH, and 1 mL of inorganic phosphorus-d...

Embodiment 3

[0104] Example 3 Mechanism of Phosphorus Solubilizing Phosphorus Solubilizing Bacteria in Rhizosphere of Wangtianshu

[0105] 3.1 Test method

[0106] 3.1.1 Determination of Phosphorus Solubilizing Ability of Phosphorus Solubilizing Bacteria and pH of Bacteria Solution

[0107] The test strain was activated on beef extract peptone solid medium at 30°C for 24h, inoculated a ring of activated bacteria into 30mL LB liquid medium, and cultured on a shaking table for 24h at a speed of 180r / min and a temperature of 30°C. Draw 1mL of the seed liquid of each bacterial strain that was cultivated overnight and inoculate them into 30mL of organic phosphorus and inorganic phosphorus liquid medium respectively (organophosphorus decomposing bacteria are inoculated in organic phosphorus liquid medium, inorganic phosphorus decomposing bacteria are inoculated in inorganic phosphorus liquid medium), Set up 3 replicates for each treatment, culture at 30°C, 180r / min shaking for 7 days, regularly...

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Abstract

The invention discloses Burkholderia gladioli P8 and application thereof, and belongs to the technical field of microorganisms, the Burkholderia gladioli P8 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 13, 2021, and the preservation registration number is CGMCC No.22825; the invention shows that the strain has the best inorganic phosphorus dissolving effect when the temperature is 35 DEG C, the pH is 7.5, the carbon source is glucose, the nitrogen source is ammonium sulfate, the C / N ratio is 40: 1 and the salt concentration is 1.5%, and the strain can secrete phosphatase and phytase, dissolve insoluble organic phosphorus and secrete plant hormones and siderophores to promote photosynthesis, respiration and substance and energy metabolism of plants, the invention can be used for solving the problems of slow seedling root development and slow seedling growth of the tamarind.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Burkholderia gladiolus P8 and its application. Background technique [0002] Phosphorus plays an important role in plant growth. It is the main nutrient element in the metabolic processes of plant energy transfer, signal transduction, macromolecular biosynthesis, photosynthesis and respiration. At the same time, phosphorus element is also an important nutrient element to promote root growth and development. Its deficiency severely restricts the growth of plants. Acidic soil itself has a high phosphorus content, but because acidic soil has a strong solidification effect on phosphorus, most of the phosphorus in the soil is combined with calcium, aluminum or iron minerals to form insoluble phosphate, which in turn makes the effective phosphorus content in the soil Very low, only a small amount of available phosphorus can be provided to plants. On average, most o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C05F11/08C12R1/01
CPCC12N1/20A01N63/20C05F11/08
Inventor 杨梅韩小美余注光黄平升李万年汪丛啸廖思
Owner GUANGXI UNIV
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