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Nitrogen-containing organic acid in purslane and extraction and separation method thereof

A separation method, the technology of purslane, applied in the direction of organic active ingredients, carboxylic acid amide separation/purification, food ingredients containing natural extracts, etc., can solve the problem of low structural novelty, and achieve environmentally friendly process methods and simple operation methods and fast results

Active Publication Date: 2022-02-11
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of new compounds in purslane are urgently needed

Method used

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  • Nitrogen-containing organic acid in purslane and extraction and separation method thereof
  • Nitrogen-containing organic acid in purslane and extraction and separation method thereof
  • Nitrogen-containing organic acid in purslane and extraction and separation method thereof

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Effect test

Embodiment 1

[0035] The invention provides a nitrogen-containing organic acid compound, the molecular formula is C 16 h 30 N 2 o 5 , named 5-(diethylamino)-2-(3-(diethylamino)-3-oxopropyl)-2-hydroxy-5-oxopentanoic acid. The chemical structural formula is:

[0036] .

[0037] The nitrogen-containing organic acid compound is named 5-(diethylamino)-2-(3-(diethylamino)-3-oxopropyl)-2-hydroxy-5-oxopentanoic acid according to the structure, and Table 1 is the nitrogen-containing organic acid compound NMR data: 1 H-NMR with 13 C-NMR in MeOD- d 4 middle.

[0038] Table 1: NMR data of nitrogen-containing organic acid compounds of the present invention

[0039] .

[0040] Structural identification and deduction of nitrogen-containing organic acid compound 5-(diethylamino)-2-(3-(diethylamino)-3-oxopropyl)-2-hydroxy-5-oxopentanoic acid of the present invention.

[0041] The obtained compound was a pale yellow powder, which was easily soluble in methanol. HRESI(-)TOFMS gives m / z: 230.139...

Embodiment 2

[0049] Example 2 The anticholinesterase effect of the nitrogen-containing organic acid compound of the present invention.

[0050] 1. Main materials.

[0051] 1.1. Drugs and reagents: The nitrogen-containing organic acid compounds used in the experiment were prepared by the above method, with a purity of 90-99%, sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Technology), phosphorus 5,5'-dithiobis(2-nitrobenzoic acid) (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and iodide thioacetylcholine (Acetylthiocholine iodide, ATCI, Dalian Meilun Biotechnology Co., Ltd).

[0052] 1.2. Grouping: divided into negative control group, positive control group and experimental group, each group.

[0053] 2. Experimental method.

[0054] 2.1 Sample preparation: Accurately weigh the sample and 1 mg of physostigmine, and use methanol as the solvent to prepare five gradients of 1 mg / mL, 0.5 mg / ...

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Abstract

The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a nitrogen-containing organic acid compound extracted, separated and identified from purslane and an extraction and separation method thereof. The molecular formula of the nitrogen-containing organic acid compound is C16H30N2O5, and the nitrogen-containing organic acid compound is named as 5-(diethylamino)-2-(3-(diethylamino)-3-oxopropyl)-2-hydroxy-5-oxopentanoic acid. The invention also provides the extraction and separation method of the nitrogen-containing organic acid compound. The nitrogen-containing organic acid compound is prepared by sequentially adopting water decoction extraction, silica gel column chromatography, ethyl acetate extraction, ODS medium-pressure column, SI medium-pressure column and liquid phase separation. The structure of the nitrogen-containing organic acid compound is determined to be the nitrogen-containing organic acid compound by adopting 1H-NMR, 13C-NMR and two-dimensional nuclear magnetic spectrum analysis methods. The compound has potential anti-cholinesterase activity, the preparation method is provided, and a primer and a theoretical basis are provided for developing new drugs and new components.

Description

technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to nitrogen-containing organic acid compounds extracted, separated and identified from purslane medicinal materials and an extraction and separation method thereof. Background technique [0002] purslane( Portulaca oleracea L.), also known as longevity vegetable, horse amaranth, five-element grass, is an annual succulent herb of the family Portulaca oleracea. Purslane is drought and waterlogging resistant, light and shade tolerant, widely distributed and rich in resources. Most of them are wild, and few people plant them. It is one of the 78 kinds of wild plants with the same origin of medicine and food stipulated by the Ministry of Health of my country. The 2020 edition of "The Pharmacopoeia of the People's Republic of China" records that the dried aerial part of purslane is used as medicine. It has the effects of clearing away heat and d...

Claims

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Application Information

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IPC IPC(8): C07C235/06C07C231/24A61K31/194A61P43/00A23L33/105
CPCC07C235/06C07C231/24A61K31/194A61P43/00A23L33/105A23V2002/00A23V2200/30A23V2250/02A23V2250/21A23V2300/02A23V2300/14A23V2300/38A23V2300/50Y02A50/30
Inventor 成泽东英锡相郭胜男田佳音
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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