Method for measuring cysteine, homocysteine and glutathione
A homocysteine and glutathione technology, which is applied in the fields of nanomaterial preparation and chemical analysis and detection, can solve problems such as limited application, and achieve the effects of excellent sensitivity, improved detection efficiency, and widened application scope.
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Embodiment 1
[0038] 1.1 Synthesis of gold nanoclusters (GSSG-AuNCs)
[0039] 100mL containing 10mM oxidized glutathione (GSSG) and 2mM chloroauric acid (HAuCl 4 ) was poured into a round-bottomed flask, a condensing reflux device was installed, the power of the microwave reactor was set to 350W, the heating mode was controlled to be turned on for 20s and stopped for 10s, and the heating time was 60min.
[0040] After the reaction solution is cooled to normal temperature, put it in a centrifuge tube, centrifuge at 4000rpm for 20min, pour off the upper layer solution, add an equal amount of deionized water, mix well, centrifuge at 4000rpm for 20min, wash with deionized water three times, The gold nanoclusters were poured into a porcelain crucible, placed in a vacuum drying oven, and after vacuuming, the temperature of the drying oven was set at 120°C and dried for 8 hours.
[0041] 1.2 Determination of gold element content in gold nanoclusters
[0042] Accurately weigh 0.01g of gold nanocl...
Embodiment 2
[0050] This embodiment synthesizes manganese dioxide nanosheets (MnO 2 -NSs), and their composition and morphology were characterized.
[0051] 2.1 MnO 2 -NSs synthesis
[0052] 1.0 mL of KMnO at a concentration of 10 mM 4 The solution was added to 2.0 mL of a 50 mM MES (2-morpholineethanesulfonic acid) solution and diluted to 10 mL with deionized water. The mixture was sonicated for 30 min until the mixture became a brown gel. Subsequently, MnO was obtained by centrifugation at 8000 rpm for 15 min 2 Nanosheets. Then, the product was washed five times with deionized water. MnO 2 -NSs was poured into a porcelain crucible, placed in a vacuum drying oven, and after vacuuming, the temperature of the drying oven was set at 120°C and dried for 8 hours.
[0053] 2.2 MnO 2 -Determination of manganese content in NSs
[0054] Accurately weigh 0.01g MnO 2 -NSs, put into a cleaned 30mL polytetrafluoroethylene digestion tank (Xi'an Instrument, Shaanxi Province, China), then add ...
Embodiment 3
[0061] In this embodiment, the pH value of the measurement solution is optimized.
[0062] 3.1 Synthesis of GSSG-AuNCs
[0063] Pour 50mL of a mixed solution containing 10mM oxidized glutathione and 2mM chloroauric acid into a round-bottomed flask, install a condensation reflux device, set the power of the microwave reactor to 200W, control the heating mode to every 20s and stop for 10s, and heat The time is 60 minutes.
[0064] After the reaction solution is cooled to room temperature, place it in a centrifuge tube, centrifuge at 8000rpm for 10min, pour off the upper layer solution, add an equal amount of deionized water, mix well, centrifuge at 8000rpm for 10min, and wash with deionized water three times. The gold nanoclusters were poured into a porcelain crucible, placed in a vacuum drying oven, and after vacuuming, the temperature of the drying oven was set at 120°C and dried for 8 hours.
[0065] 3.2 Determination and characterization of gold content in GSSG-AuNCs
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