Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel crown RBD protein fluorescent microsphere composite preparation

A technology of composite preparation and fluorescent microspheres, applied in the field of saturated competition, can solve the problems of affecting the performance of detection reagents and the inability to perform detection, and achieve the effect of improving detection sensitivity and maintaining antigen activity.

Active Publication Date: 2022-03-01
DYNAMIKER BIOTECH TIANJIN
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stability of the fluorescent microspheres plays a key role in the subsequent detection steps. If the fluorescent microspheres are not properly stored, subsequent detection will not be possible, which will affect the performance of the final detection reagent.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel crown RBD protein fluorescent microsphere composite preparation
  • Novel crown RBD protein fluorescent microsphere composite preparation
  • Novel crown RBD protein fluorescent microsphere composite preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A new crown RBD protein fluorescent microsphere composite preparation, the preparation method is as follows:

[0053] (1) Weigh 10mg ovalbumin and add it to phosphate buffered saline (0.02-0.1M PBS, pH 7.2-7.4), then add 10-50mg / mL 1-ethyl-(3-dimethylaminopropyl ) Carbodiimide (EDC), avoid light reaction for 15-30min;

[0054] (2) Weigh 10 mg of bovine serum albumin and 10 mg of hemocyanin into the activated ovalbumin solution;

[0055] (3) Dialyze with phosphate buffered saline (0.02-0.1M PBS, pH 7.2-7.4) to remove EDC;

[0056] (4) After affinity purification, the product whose molecular weight was the sum of the molecular weights of the three proteins was collected to obtain coupling protein 1 (BSA-OVA-KLH);

[0057] (5) Weigh 2.5g trehalose, 0.9g NaCl, 0.05g PEG4000, 0.05g Tween 20, 0.05gproclin 300, 0.24g Tris, 100g process water, add coupling protein 1 to make the final concentration 1%, adjust the pH to 8.5, to obtain the compound preparation.

Embodiment 2

[0059] A new crown RBD protein fluorescent microsphere composite preparation, the preparation method is as follows:

[0060] (1) Weigh 10mg bovine serum albumin and add it to phosphate buffered saline (0.02-0.1M PBS, pH 7.2-7.4), then add 10-50mg / mL 1-ethyl-(3-dimethylaminopropyl base) carbodiimide (EDC), avoid light reaction for 15-30min;

[0061] (2) Weigh 10 mg of silk fibroin and add it to the activated bovine serum albumin solution;

[0062] (3) Dialyze with phosphate buffered saline (0.02-0.1M PBS, pH 7.2-7.4) to remove EDC;

[0063] (4) After affinity purification, the product whose molecular weight was the sum of the molecular weights of the two proteins was collected to obtain coupling protein 2 (BSA-SF).

[0064] (5) Weigh 2.5g trehalose, 0.9g NaCl, 0.05g PEG4000, 0.05g Tween 20, 0.05gproclin 300, 0.24g Tris, 100g process water, add coupling protein 2 to make the final concentration 1%, adjust the pH to 8.5, to obtain the compound preparation.

Embodiment 3

[0066] A new crown RBD protein fluorescent microsphere composite preparation, the preparation method is as follows:

[0067] (1) Weigh 10mg of polylysine and add it to phosphate buffer (0.02-0.1M PBS, pH 7.2-7.4), then add 10-50mg / mL 1-ethyl-(3-dimethylaminopropyl base) carbodiimide (EDC), avoid light reaction for 15-30min;

[0068] (2) Weigh 10 mg bovine serum albumin and add it to the activated polylysine solution;

[0069] (3) Dialyze with phosphate buffered saline (0.02-0.1M PBS, pH 7.2-7.4) to remove EDC;

[0070] (4) After affinity purification, the product with higher protein concentration was collected to obtain coupling protein 3 (ε-PL-BSA);

[0071] (5) Weigh 5g sucrose, 1.3g MgCl 2 , 0.05g PEG4000, 0.02g Tetronin1307, 0.02g KroVin300M, 0.24g Tris, 100g process water, add coupling protein 3 to make the final concentration 1%, and adjust the pH to 8.5 to obtain a compound preparation.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a novel crown RBD protein fluorescent microsphere composite preparation. The composite preparation is prepared from the following raw materials in percentage by mass: 0.5 to 1 percent of coupling protein, 2.5 to 5 percent of saccharide, 0.9 to 1.3 percent of ion, 0.05 to 0.07 percent of macromolecular substance, 0.05 to 0.1 percent of surfactant, 0.24 to 1.21 percent of buffer solution and 0.02 to 0.05 percent of preservative. The novel crown RBD protein fluorescent microsphere composite preparation can be used for preservation, dilution and treatment of novel crown RBD protein fluorescent compounds and biological specimens. When neutralizing antibodies in human serum, plasma and whole blood samples are detected by a saturation competition method, a time-resolved fluorescent microsphere compound coupled with new crown RBD protein needs to be independently stored, and the compound preparation can prevent the fluorescent microsphere compound from generating aggregation and precipitation, keep the antigen activity and improve the detection sensitivity. The biological specimen comprises serum and plasma.

Description

technical field [0001] The invention belongs to the field of saturated competition, and in particular relates to a composite preparation of novel coronavirus RBD protein fluorescent microspheres. Background technique [0002] In the direct competition method in immunology, the labeled antigen and the antigen to be tested are both in liquid phase, and the chance of binding to the antibody is the same, and the relative binding rate of the labeled antigen is 50%. In the saturation competition method, the contact area between the solid-phase antigen and the antibody is small, and only the remaining antibody that binds to the antigen to be tested will bind to the solid-phase antigen, and the relative binding rate of the solid-phase antigen is 0%. Therefore, the slope of the inhibition curve of the indirect method will be greater than that of the direct competition method. The high sensitivity of the saturation competition method is sometimes difficult to achieve in ELISA, and th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 王丽娇彭洁张变强于迪盛长忠粟艳周泽奇
Owner DYNAMIKER BIOTECH TIANJIN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com