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Application of 7-methylxanthine as detection target in preparation of type 2 diabetes mellitus high-risk individual screening kit

A technology of methylxanthine and type 2 diabetes, which is applied in the application field of preparing a screening kit for high-risk individuals of type 2 diabetes, can solve the problems of information lag, low sensitivity and specificity, and limited screening technology, and achieve good results. The effect of predictive value

Active Publication Date: 2022-03-04
无锡市疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far in the practice of prevention and control, the screening technology for high-risk individuals with type 2 diabetes is limited. It can only be based on traditional risk factors such as age, obesity, diabetes family history, blood sugar, and blood lipids. There are low sensitivity and specificity, information bias, and information Hysteresis and other deficiencies

Method used

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  • Application of 7-methylxanthine as detection target in preparation of type 2 diabetes mellitus high-risk individual screening kit
  • Application of 7-methylxanthine as detection target in preparation of type 2 diabetes mellitus high-risk individual screening kit
  • Application of 7-methylxanthine as detection target in preparation of type 2 diabetes mellitus high-risk individual screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Collection of research samples and data arrangement

[0024] From April to June 2007, the inventor collected 10,867 morning fasting blood samples from local residents aged 35 and above from Jianghai Community and Beidajie Community, Liangxi District, Wuxi City, centrifuged at 4000r / min immediately after collection, and took 1.5ml serum Aliquot into cryopreservation tubes and store in -80°C refrigerator; complete epidemiological questionnaire survey (including demographic and sociological information, behavior and lifestyle, disease history and family history), physical examination (measurement of height, weight, waist circumference, blood pressure) at the same time ). Through passive follow-up and active follow-up, find out whether type 2 diabetes occurs.

[0025] (1) Type 2 diabetes case group: the fasting blood glucose was less than 7.0mmol / L at the baseline survey, without diabetes, cardiovascular and cerebrovascular diseases, and tumor history, and was di...

Embodiment 2

[0027] Example 2 Analysis of differential metabolic profiles between type 2 diabetes cases and matched controls

[0028] The above-mentioned 220 cases of type 2 diabetes and 220 healthy controls were tested by metabolomics to obtain relevant results. The specific experimental method is as follows:

[0029] 1.1 Instrument

[0030] UPLC Ultimate 3000system (Dionex) high-performance liquid chromatography; Q-Exactive triple quadrupole tandem mass spectrometer; TraceFinder 3.1 (Thermo Fisher Scientific); Simca P 13.0 (Umetrics, Sweden); XW-80A vortex mixer (Shanghai Qingpu Shanghai) West Instrument Factory); electronic balance (MettlerAE240); KQ3200B ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0031] 1.2 Reagents

[0032] 1579 metabolite standards (≥98.0%, Sigma–Aldrich, St.Louis, MO, USA); methanol, acetonitrile (≥99.9%, Merck, German); formic acid (≥98.0%, Sigma–Aldrich, St.Louis , MO, USA); n-hexane (chromatographic grade, ≥98.0%; chloroform (≥99.9%); ult...

Embodiment 3

[0046] Example 3 Production of 7-methylxanthine detection kit for screening high-risk individuals with type 2 diabetes

[0047] This kit includes reagents and consumables for 7-methylxanthine detection, of which 7-methylxanthine standards are used for qualitative and quantitative analysis. Others include supporting reverse chromatographic columns for UPLC chromatographic separation (Hypersil GOLD C18 chromatographic column, 100mm×2.1mm, particle size 1.9μm), reagents for precipitating plasma proteins (100% methanol), mobile phase Reagents (0.1% formic acid in water and 0.1% formic acid in acetonitrile), reagents for extracting 7-methylxanthine (100% ultrapure water). The value of this kit is that only 100 μl of serum is needed to detect the content of 7-methylxanthine, and then screen high-risk individuals for type 2 diabetes through the content.

[0048] The specific kit composition is as follows:

[0049] 7-Methylxanthine Standard

[0050] Chromatographic column (Thermo 1...

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Abstract

The invention discloses an application of 7-methylxanthine as a detection target in preparation of a kit for screening high-risk individuals of type 2 diabetes mellitus. The 7-methylxanthine is used as a detection target in preparation of a serum / plasma auxiliary screening reagent for high-risk individuals suffering from type 2 diabetes mellitus. The invention discloses application of a reagent for quantitatively detecting 7-methylxanthine in preparation of a serum / plasma auxiliary screening reagent for type 2 diabetes mellitus. According to the application disclosed by the invention, the increase of 7-methylxanthine is a metabolic molecule with high specificity and sensitivity, which is highly related to the morbidity of the type 2 diabetes mellitus in the future, and the AUC of the 7-methylxanthine for independently predicting the type 2 diabetes mellitus is 0.768; the AUC of the type 2 diabetes mellitus predicted by the traditional risk factor is 0.797; after the metabolite 7-methylxanthine is added, the AUC is increased to 0.880, and a very good prediction value is achieved on the occurrence of the type 2 diabetes mellitus; and a technical support is provided for early screening of high-risk individuals of type 2 diabetes mellitus.

Description

[0001] field of invention [0002] The invention belongs to the fields of metabonomics, public health and preventive medicine, and relates to the application of 7-methylxanthine as a detection target in the preparation of a screening kit for high-risk individuals with type 2 diabetes. Background technique [0003] Diabetes is a major chronic disease that seriously affects people's life and health. The number of morbidity and deaths is increasing, and it is showing a younger trend. The prevalence rate of adults aged 18 and over in my country has soared from 0.67% in 1980 to 11.2% in 2017; age standardization Mortality rate is on the rise. Diabetes can cause serious complications such as stroke, coronary heart disease, blindness, foot gangrene, disability and high mortality. Among diabetes, more than 90% are type 2 diabetes (Type 2Diabetes). Early detection of high-risk individuals with type 2 diabetes and intervention can effectively reduce the occurrence of type 2 diabetes an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N30/88
CPCG01N33/50G01N30/88G01N2030/8836
Inventor 钱云刘佳王璐
Owner 无锡市疾病预防控制中心
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