Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of citrobacter freundii in efficient degradation of DMAc

A technology of citric acid and bacillus freundii is applied in the field of microorganisms, which can solve the problems such as difficulty in stabilizing the water quality of the effluent, and achieve the effects of low cost and simple operation.

Pending Publication Date: 2022-03-15
GUIZHOU MATERIAL IND TECH INSTITUE
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional biological treatment method is greatly affected by factors such as the environment and microbial growth characteristics, and the effluent quality is difficult to stabilize

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of citrobacter freundii in efficient degradation of DMAc
  • Application of citrobacter freundii in efficient degradation of DMAc

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Experimental steps:

[0020] The ratio of LB medium is: NaCl 1%, peptone 1%, yeast extract powder 0.5%, agar 2%-3%.

[0021] 1. Collect about 10g of sludge from the drainage ditch of the membrane production plant, add it to 100ml of sterile water, and incubate at 35°C and 150rpm for about 4 hours to prepare a bacterial suspension.

[0022] 2. Dilute the bacterial suspension by 10 -1 、10 -2 、10 -3 、10 -4 After dilution, they were respectively spread on LB medium, and then placed in an incubator for 48 hours of cultivation at a temperature of 37°C. The ratio of LB medium is: NaCl 1%, peptone 1%, yeast extract powder 0.5%, agar 2%-3%.

[0023] 3. Pick colonies of different shapes from the petri dishes in step 2, streak on LB medium, and culture at 37°C for 48 hours.

[0024] 4. Repeat step 3.

[0025] 5. Separate colonies of different shapes by streaking in steps 3 and 4, inoculate them into DMAC medium respectively, and culture at 37°C and 150rpm for 24h. The rati...

Embodiment 2

[0035] Utilize the Citrobacter freundii screened out of the present invention to carry out DMAC degradation experiment, concrete steps are as follows:

[0036] 1. Pick Citrobacter freundii from a refrigerated inclined tube and inoculate it into 100 mL of LB liquid medium, and culture it at about 37°C and 150 rpm for 24 hours to prepare the seed solution;

[0037] 2. Take 1mL seed solution and inoculate it into 100mL inorganic salt liquid medium containing DMAC. The formula of inorganic salt medium is: Na 2 HPO 4 2H 2 O 2.0g L -1 , MgSO 4 0.5gL -1 , KCl 0.5g L -1 , KH 2 PO 4 1.0gL -1 , Fe 2 (SO4) 3 ·H 2 O0.2gL -1 , CaCl 2 2H 2 O 0.1g L -1 . The initial concentration of DMAC was 0.5mg / L. After 24 hours of shaking culture at 37°C and 150rpm, the DMAC concentration was 0.37mg / L by sampling, 0.11mg / L by sampling at 48h, and 0.03 after 72h. mg / L.

[0038] The results showed that: Citrobacter freundii had a good degradation effect on DMAC.

Embodiment 3

[0040] Utilize the Citrobacter freundii screened by the present invention to simulate the waste water treatment experiment containing DMAC fee, the concrete steps are as follows:

[0041] 1. Pick Citrobacter freundii from a refrigerated inclined tube and inoculate it into 100 mL of LB liquid medium, and culture it at about 37°C and 150 rpm for 24 hours to prepare the seed solution;

[0042] 2. The MBR wastewater treatment simulation device with a capacity of 200L (see figure 2 ), configure 120L of DMAC solution according to the concentration of 500mg / L, add polyurethane biological filler, turn on the aeration pump, and the aeration rate is 20L / min.

[0043] 3. Pour 100mL of seed solution and 1L of inorganic salt culture medium into the wastewater, and the wastewater is continuously aerated. After a certain period of time, 100mL of water samples are taken through the hollow fiber membrane module, and the DMAC concentration is measured. The measurement results are shown in Tabl...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of citrobacter freundii in efficient degradation of DMAc (dimethylacetamide). Belongs to the technical field of microorganisms. Aiming at the defects of a traditional biological treatment method, the citrobacter freundii can efficiently degrade DMAc through screening, and the citrobacter freundii has the advantages of being easy to operate, low in cost, environmentally friendly and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of Citrobacter freundii in efficiently degrading DMAc. Background technique [0002] DMAc (N, N-dimethylacetamide) is a colorless and transparent aprotic highly polar solvent with strong solubility and can be mixed with water, aromatic compounds, esters, ketones, alcohols, ethers, benzene and trichloro As an important chemical raw material, it is widely used in many industries such as spinning, medicine, pesticide, organic raw material, petrochemical industry, etc. [0003] However, due to its wide and large application, the discharge of DMAc industrial wastewater is also increasing day by day, and the pollution of DMAc is becoming more and more serious, which brings great pressure to the environment and ecosystem. According to the existing research results, DMAc has a certain toxic effect on animals and humans, DMAc has a stimulating effect on the human r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/38
CPCC02F3/34C02F2101/38
Inventor 李科褡杨园园雷婷李剑姚勇张敏敏杨敬葵武晓
Owner GUIZHOU MATERIAL IND TECH INSTITUE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com