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Preparation method of extremely thermophilic microbial agent and application of extremely thermophilic microbial agent in municipal sludge composting

A technology of thermophilic microorganisms and extreme thermophilic bacteria, applied in the field of microorganisms, can solve the problems of long fermentation cycle, slow heating speed, limited maximum fermentation temperature, etc. Effect

Pending Publication Date: 2022-03-22
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the technical problems of long municipal sludge composting fermentation period, slow heating rate, and limited maximum fermentation temperature, the invention provides a preparation method of an extreme thermophilic microbial bacterial agent, and the prepared extreme thermophilic microbial bacterial agent can promote Increase the temperature of urban sludge composting, increase the temperature in the high temperature stage to above 75°C, and effectively prolong the duration of the high temperature stage of composting

Method used

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  • Preparation method of extremely thermophilic microbial agent and application of extremely thermophilic microbial agent in municipal sludge composting
  • Preparation method of extremely thermophilic microbial agent and application of extremely thermophilic microbial agent in municipal sludge composting
  • Preparation method of extremely thermophilic microbial agent and application of extremely thermophilic microbial agent in municipal sludge composting

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation method of extreme thermophilic microbial bacterial agent

[0048] 1. Take water samples from the hot spring in Liujiazhai, Tengchong, Yunnan. The temperature of the hot spring is 80°C. Use a sterile sampler to draw hot spring water samples into a sterile water sample collection bag, store them in a dry ice box at low temperature, and transport them to the laboratory for subsequent processing.

[0049] 2. Enrichment of extreme thermophagic microorganisms

[0050] Selection of the most suitable medium:

[0051] Seven kinds of microbial enrichment media commonly used in laboratories were selected to enrich microorganisms in water samples from hot springs in Tengchong, Yunnan. include:

[0052] (1) Peptone water medium: peptone 1.0g, K2 HPO 4 0.2g, Na 2 HPO 4 0.2g, NaCl 0.5g, glucose 0.5g, add deionized water to 1000mL, adjust pH to 7.8; (2) yeast extract medium: yeast extract 10.0g, add deionized water to 1000mL, pH Natural; (3) Wort medium: malt extra...

Embodiment 2

[0064] Application of extreme thermophagic microorganism bacterial agent

[0065] 1. Test material

[0066] Table 2 High temperature composting raw material moisture content

[0067]

[0068] The raw sludge used in the laboratory-scale sludge composting experiment was provided by the Sanshui Plant of Changsha Water Supply Company. The auxiliary materials used included rice husk, rice bran, bran and sawdust. The moisture content of each raw material and the physical and chemical properties of the raw sludge are shown in Tables 2 and 3. .

[0069] Table 3 Physical and chemical properties of raw sludge

[0070]

[0071] After the moisture content of each raw material is determined, the four auxiliary materials are mixed according to the ratio of 0.5:1.5:1:1 of rice husk, rice bran, bran and sawdust, and the addition ratio of mixed auxiliary materials and dewatered sludge (ie raw sludge) is calculated , control the moisture content of the composting system at about 60%, a...

Embodiment 3

[0103] Isolation and Characterization of Extremophilic Microorganisms

[0104] 1. Culture medium: The enrichment medium is nutrient broth (NB) medium and CYS medium (see Tables 4 and 5 for ingredients).

[0105] Table 4 CYS medium composition (pH7.5)

[0106]

[0107] Table 5 Composition of trace elements[40]

[0108]

[0109] The plate used is made of the above medium plus 1.5-2% agar. The culture can be used after steam sterilization at 121°C for 20 minutes.

[0110] 2. Enrichment and isolation of thermophilic microorganisms

[0111] Take 10mL of the fermented compound extreme thermophilic microorganism bacterial agent into 100mL NB and CYS medium respectively, place it on a shaker at 75°C, enrich and cultivate it at 170rpm for 72h, and inoculate the enriched bacterial suspension to In the newly prepared NB and CYS medium, after subculture and enrichment for 3 times, single bacteria were isolated by concentration gradient dilution method and plate streak method, re...

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Abstract

The invention provides a preparation method of an extreme thermophilic microbial agent, and belongs to the technical field of microorganisms, the preparation method comprises the following steps: inoculating a culture medium with hot spring water at 75-85 DEG C, and culturing at 75 DEG C to obtain a microbial seed solution; adding a protective agent into the microbial seed solution, then adding carrier powder according to a solid-to-liquid ratio of 1: 1.5, and stirring at 75 DEG C for 2 hours to obtain a microbial mixed solution; and concentrating the microbial mixed solution, and drying at 75 DEG C to obtain the extreme thermophilic microbial agent. The prepared extreme thermophilic microbial agent can promote the temperature rise of municipal sludge compost, increase the temperature of a high-temperature stage to 75 DEG C or above, and effectively prolong the duration of the high-temperature stage of compost. The invention also provides an application of the extremely thermophilic microbial agent in municipal sludge composting.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a preparation method of an extreme thermophilic microbial bacterial agent and its application in urban sludge composting. Background technique [0002] The activated sludge method is currently the most widely used urban sewage treatment technology. The large amount of excess sludge produced by this method has become a major problem for sewage treatment plants. Composting is one of the most economical and effective ways of recycling and harmless treatment of urban sludge. This method utilizes microorganisms that exist widely in nature to promote the conversion of biodegradable organic matter in solid waste into stable and complex structures in a controlled manner. Humus, which can completely decompose and utilize organic matter, without generating intermediate products, and has a high degree of harmlessness. Aerobic composting is currently the first choice for most compostin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/20C12R1/07C12R1/10C12R1/01
CPCC12N1/20C05F17/20C05F11/08Y02W30/40
Inventor 尹华群孟德龙谭格
Owner CENT SOUTH UNIV