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Composite hydrogel for promoting wound healing and preparation method and application thereof

A composite hydrogel and wound healing technology, applied in medical science, bandages, etc., to achieve high biological activity, accelerate healing, and promote healing

Pending Publication Date: 2022-03-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many conventional hydrogels use harsh chemicals during the gelation process, which can limit the incorporation of bioactive molecules or cells

Method used

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  • Composite hydrogel for promoting wound healing and preparation method and application thereof
  • Composite hydrogel for promoting wound healing and preparation method and application thereof
  • Composite hydrogel for promoting wound healing and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for preparing silk fibroin methacryl (SFMA), comprising the following steps:

[0032] Submerge 10g of cocoons in 1L of 0.05M Na 2 CO 3 The solution was boiled at 100°C for 30 minutes, and washed several times with distilled water to obtain degummed silk fibroin. Then, the degummed silk fibroin (SF) was dried in a drying oven for 36 hours. To obtain SFMA, 10 g of degummed SF was dissolved in 9.3 M lithium bromide (LiBr) solution at 60 °C for 1 h, stirred with a magnetic stirrer, and then 6 mL of glycidyl methacrylate (GMA) was slowly added dropwise. After 8 hours of reaction, the solution was filtered through a magic filter cloth to remove salt, and dialyzed with distilled water for 7 days using a dialysis bag. The resulting SFMA solution was frozen at −80 °C for 12 h and then freeze-dried for 36 h to obtain spongy SFMA.

[0033] In fact, the ratio of the weight of the degummed silk fibroin to the volume of the glycidyl methacrylate can be 1: (0.3-0.9) g / ml....

Embodiment 2

[0035] A method for preparing dextran methacryl (DEXMA), comprising the following steps:

[0036] Dissolve 10 g of dextran in 100 mL of anhydrous DMSO at room temperature and stir for 1 h. Then 2 g of 4-dimethylaminopyridine (DMAP) was added to the above solution. After stirring for 1 hour, 3 mL of glycidyl methacrylate (GMA) was added. The reaction was carried out for about 48 h at room temperature under closed, dark and continuous stirring. The reaction mixture was then dialyzed against a dialysis bag for about 3 days. The resulting DEXMA solution was frozen at -80°C for 12 hours and then freeze-dried for 36 hours to obtain sponge-like DEXMA.

[0037] In fact, the weight ratio of the dextran to the glycidyl methacrylate may be 1: (0.1-0.6) g / ml.

Embodiment 3

[0039] The preparation method of 10%SFMA / 1%DEXMA hydrogel comprises the following steps:

[0040] The SFMA (10wt.%) prepared by 0.2g embodiment 1 and the DEXMA (1wt.%) prepared by 0.02g embodiment 2 and 0.002gLAP photoinitiator (0.1wt.%) were dissolved in 2mL deionized water, and the ultraviolet light was irradiated for 30s , namely 10% SFMA / 1% DEXMA hydrogel.

[0041] In fact, based on the volume of deionized water, the amount of methacrylylated silk fibroin SFMA can be 5w / v%-15w / v%, and the above-mentioned ultraviolet irradiation time can be 10-120s.

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Abstract

The invention provides composite hydrogel for promoting wound healing as well as a preparation method and application of the composite hydrogel. The composite hydrogel for promoting wound healing comprises a hydrogel matrix as well as platelet-rich plasma and an acellular dermal matrix which are loaded on the hydrogel matrix, and the hydrogel matrix is formed by cross-linking methacrylated silk fibroin and methacrylated glucan; the preparation method comprises the following steps: adding the methacrylated silk fibroin, the methacrylated glucan, the acellular dermal matrix and the photoinitiator into deionized water, dissolving to form a mixture, then adding blood plasma rich in platelets, and then carrying out photo-crosslinking, thereby obtaining the photocrosslinked silk fibroin / acellular dermal matrix composite. The composite hydrogel disclosed by the invention can be used as a medical dressing, can realize slow release of growth factors, has relatively high biological activity, and has a remarkable potential for promoting healing of diabetic wounds.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a composite hydrogel for promoting wound healing and its preparation method and application. Background technique [0002] The high prevalence of diabetes worldwide is a major problem that adversely affects the health of patients. Complications such as diabetic wounds have become a significant cause of disability worldwide, placing a huge burden on patients and resulting in poor quality. Microvascular dysfunction and cytokine distribution imbalance caused by high glucose load may be considered as the main reasons for chronic, delayed or even non-healing wounds in diabetic patients. Wounds that don't heal are a huge challenge for clinics, leading to greater risks of gangrene, amputation and even death. Therefore, improving angiogenesis and revascularization in diabetic non-healing wounds is crucial to accelerate chronic wound healing. In general, surgic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C08J3/075C08L89/00C08L5/02C08K11/00
CPCA61L26/008A61L26/0095A61L26/0066A61L26/0057C08J3/075A61L2300/30A61L2300/412C08L89/00C08L5/02
Inventor 郭瑞冯龙宝周莉明
Owner JINAN UNIVERSITY
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