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Sequence for determining escherichia coli gene by using nanogold-graphdiyne composite material modified electrode

A technology of nanocomposite materials and modified electrodes, which is used in analytical materials, determination/inspection of microorganisms, material analysis by electromagnetic means, etc.

Pending Publication Date: 2022-04-05
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although electrochemical sensing technology has made a lot of progress, it is still one of the challenges for researchers to design new functional materials to improve the sensitivity of sensing technology and improve its accurate capture of target analytes.

Method used

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  • Sequence for determining escherichia coli gene by using nanogold-graphdiyne composite material modified electrode
  • Sequence for determining escherichia coli gene by using nanogold-graphdiyne composite material modified electrode
  • Sequence for determining escherichia coli gene by using nanogold-graphdiyne composite material modified electrode

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Experimental program
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specific Embodiment approach 1

[0016] (1) Preparation of base electrode CILE: After fully mixing and grinding graphite powder, ionic liquid and liquid paraffin in a certain proportion, put them into a glass electrode tube with fine copper wire inserted and compact them, and then obtain ionic liquid modified carbon paste electrode ( CILE), sand to a mirror finish on polishing paper before use.

[0017] (2) Preparation of Au-GDY nanocomposite modified electrode: 8 μL of 1.0 mg / mL GDY solution was drop-coated on the surface of CILE substrate electrode, and dried at room temperature to obtain GDY / CILE modified electrode; then it was placed in 1.6 mg / mL mL HAuCl 4 The constant potential deposition was carried out in the solution, the deposition potential was -0.5 V (vs. Ag / AgCl), and the deposition time was 50 s. Then the electrode was washed and dried naturally to prepare the Au / GDY / CILE modified electrode.

[0018] (3) Immobilization of the probe ssDNA sequence and hybridization with the target ssDNA: 10 μL ...

specific Embodiment approach 2

[0019] Specific embodiment 2, characterization of Au-GDY nanocomposite modified electrode: the SEM characterization results of CILE electrode are as follows figure 1 As shown in A, the inner illustration is the microscopic surface of CILE after magnification, and the smooth and smooth surface can be seen. figure 1 B is GDY dispersed on the surface of CILE. It can be seen that although GDY is stacked together, compared with CILE with the same geometric area, the presence of GDY provides a larger specific surface area. figure 1 C is uniform Au nanoparticles loaded on the surface of GDY.

specific Embodiment approach 3

[0020] Specific embodiment 3, time and potential optimization of electrochemical deposition of gold: In potassium ferricyanide electrolyte, the deposition time and potential of gold nanoparticles were studied. figure 2 In A, as the gold deposition time increases (10 s to 50 s), the current gradually increases, and in the next 200 s, the current gradually decreases, so the maximum response current is reached at 50 s, which is regarded as the optimal gold immersion time. At the same time, electrochemical tests were carried out on the deposition potential of gold nanoparticles between -0.6 V and -0.2 V. The results show that the modified electrode has the maximum current response when the deposition potential of gold is -0.5 V, as figure 2 Shown in B. Therefore -0.5 V is the optimal deposition potential of gold.

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Abstract

The invention relates to a nanogold-graphdiyne composite material modified electrode for determining an escherichia coli gene sequence, which is characterized in that an ionic liquid modified carbon paste electrode (CILE) is used as a substrate electrode, and the nanogold (Au)-graphdiyne (GDY) composite material modified electrode is prepared by two steps of a dispensing method and a constant potential deposition method and is used as a working electrode. A scanning electron microscope (SEM) observes the morphology of the microscopic size of the GDY and the Au-GDY composite material thereof. Cyclic voltammetry (CV) results show that the Au-GDY composite material effectively improves the electrochemical performance of a modified electrode interface and improves the electron transfer rate of the electrode. The sulfydryl-modified probe ssDNA is successfully fixed on the surface of the modified electrode by utilizing the self-assembly bonding effect of gold and sulfur. Methylene blue (MB) is used as an electrochemical indicator and is used for judging a signal successfully captured by a target sequence. Results of differential pulse voltammetry (DPV) show that the Au-GDY / CILE modified electrode has a wide detection range (1.0 * 10 <-15 > mol / L-1. 0 * 10 <-7 > mol / L), a low detection limit (3.17 * 10 <-16 > mol / L) and high sensitivity (2.91 A / (M.cm < 2 >)) on gene sequence detection of escherichia coli.

Description

technical field [0001] The invention belongs to the technical field of electrode materials, and relates to a nano-gold-graphyne composite material, a preparation method thereof and detection of E. coli gene sequences. Background technique [0002] Foodborne diseases are one of the greatest challenges to human health, and their massive occurrence not only poses a serious threat to people's lives, but also brings huge economic losses. Foodborne pathogens are an important cause of many serious foodborne disease outbreaks. Escherichia coli is one of the main foodborne pathogens. The pathogenic route of Escherichia coli is mainly transmitted through the digestive tract. It is ingested into the body by eating contaminated food or water and causes diseases such as urinary tract infection, peritonitis, cholecystitis, appendicitis, and even bacteremia. Traditional detection methods for E. coli include multi-tube fermentation method, rapid disk method, PCR detection method, immunolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327C12Q1/689C12N15/11C12Q1/6816C12Q1/6869C12R1/19
Inventor 孙伟闫丽君艾益静张婧媱张思月童菀玥
Owner HAINAN NORMAL UNIV
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