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Alkane degrading functional bacterium XP4-7 and application thereof

A technology of alkanes and paracoccus, applied in the field of microorganisms, can solve the problems of relatively few researches on petroleum hydrocarbon degradation microorganisms, and achieve the effect of excellent degradation ability

Pending Publication Date: 2022-04-12
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, petroleum hydrocarbon-degrading bacteria are mainly isolated from polluted areas such as coastal areas and land, and there are relatively few studies on petroleum hydrocarbon-degrading microorganisms in open seas

Method used

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  • Alkane degrading functional bacterium XP4-7 and application thereof
  • Alkane degrading functional bacterium XP4-7 and application thereof
  • Alkane degrading functional bacterium XP4-7 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1, screening and identification of paracoccus (Paracoccus sp.) XP4-7 strain

[0023] Preparation of artificial seawater medium (MMC): Weigh 24g of sodium chloride, 7g of magnesium sulfate heptahydrate, 1g of ammonium nitrate, 0.7g of potassium chloride, 2g of potassium dihydrogen phosphate, and 3g of sodium hydrogen phosphate, dissolve them in 1000mL of ultrapure water, Adjust the pH to 7.4, sterilize with high-pressure steam at 121°C for 20 minutes, add appropriate amount of trace element mixed solution and magnesium sulfate heptahydrate solution after sterilization; trace element mixed solution: weigh 2g of calcium chloride, 50mg of ferric chloride hexahydrate, copper sulfate 0.5mg, 0.5mg of magnesium chloride tetrahydrate, 10mg of zinc sulfate heptahydrate, dissolved in 1000mL ultrapure water, and the trace element mixture was sterilized by filtering through a 0.22μm filter membrane; solution of magnesium sulfate heptahydrate: weigh 35g of magnesium sulfate he...

Embodiment 2

[0027] Embodiment 2, paracoccus (Paracoccus sp.) XP4-7 strain morphological characteristics

[0028] A single colony was streak-inoculated on the MA plate medium and cultured at 28°C for 3 days. The colony of Paracoccus sp. XP4-7 on the MA plate is round, carrot-colored and opaque, with smooth and moist surface, regular edges, no halo, raised in the middle, and 1-2 mm in diameter. see figure 1 .

Embodiment 3

[0029] Embodiment 3, the determination of paracoccus (Paracoccus sp.) XP4-7 strain degrading alkanes

[0030]Prepare artificial seawater culture medium (MMC), sterilize at 121°C for 20 minutes, divide into 40mL glass bottles, 10mL per bottle, add petroleum and diesel oil with a final concentration of 10g / L, and store at 28°C, 150rpm, in the dark Cultivate under the same conditions for 20 days, and determine the residual alkanes before and after the culture by gas chromatography-mass chromatography (GC-MS). The results showed that the degradation rates of Paracoccus sp. XP4-7 strain to total alkanes in petroleum and diesel oil were 48% and 62%, respectively, and the degradation rates of n-decane to n-pentacane in petroleum were 0.9%, respectively. 0.9%, 16.0%, 75.0%, 70.8%, 82.1%, 85.1%, 83.4%, 93.8%, 92.2%, 79.9%, 63.3%, 82.8%, 85.3%, 78.2%, 71.6%, 77.3%, 80.0% , 84.9%, 80.4%, 72.7%, 74.9%, 73.6%, 73.8%, 53.3%, 43.2%, the degradation rates of n-undecane to n-pentacane in dies...

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Abstract

The invention discloses an alkane degrading functional bacterium XP4-7 and an application thereof. The strain is Paracoccus sp. XP4-7, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.22994, and the preservation date is July 30, 2021. The invention further discloses a preparation method of the paracoccus sp. Experiments prove that the strain XP4-7 has an excellent capability of degrading alkanes, the degradation rates of total alkanes in petroleum and diesel oil are 48% and 62% respectively, and the strain XP4-7 can be widely applied to alkane degradation and petroleum pollution remediation, such as petroleum pollution ocean water remediation and the like.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an alkane-degrading functional bacterium XP4-7 and its application. Background technique [0002] At present, marine pollution is increasing day by day. As a global marine pollutant, oil has become the main type of marine pollution, causing great damage to the marine ecological environment; 70% of the oil needed by countries in the world needs to be transported by sea. Some or all of the oil flows into the sea, causing serious marine oil pollution, which directly or indirectly affects the survival and sustainable development of human beings; human beings gradually realize that marine resources are the main resources for sustainable development in this century. [0003] The key to controlling marine oil pollution lies in how to effectively deal with oil spill pollution. The commonly used methods of marine oil pollution control include physical treatment, chemical treatment ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/32C02F103/08
Inventor 吕丽娜张偲麦志茂李洁王琳
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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