A method for detecting cloxacillin drug residues in aquatic products

Abstract

The invention discloses a method for detecting cloxacillin drug residues in aquatic products, comprising the following steps: step a, preparing an extract; step b, preparing a first sample liquid; step c, preparing a second sample liquid; step d, preparing The sample liquid to be tested; step e, determining the growth inhibitory effect of the sample liquid to be tested on Pythium by turbidimetry; step f, numerical quality inspection of the growth rate; and step g, judging the result. The method of the present invention converts cloxacillin into a product with exclusive bactericidal properties against Pythium through a chemical reaction, and then uses a biological culture method to detect the product. Compared with chromatography, the analysis procedure is simple, fast, and specific. High sensitivity, high accuracy of test results, no need for large analytical instruments, relatively less time-consuming, low analysis cost, low requirements for experimental environment, low requirements for operators, suitable for testing whether cloxacillin is contained in aquatic products Daily supervision and inspection.

Description

technical field [0001] The invention relates to the field of aquatic product detection, in particular to a method for detecting cloxacillin drug residues in aquatic products. Background technique [0002] Cloxacillin is a semisynthetic penicillinase-resistant penicillin. Its antibacterial effect is characterized by: high stability to penicillinase (its stability is higher than other penicillinase-resistant penicillin drugs), antibacterial to penicillinase-producing Staphylococcus (including Staphylococcus aureus and coagulase-negative staphylococcus) The activity is stronger than that of oxacillin, but its antibacterial effect on penicillin-sensitive staphylococci and various streptococci is 5-10 times weaker than that of penicillin. Cloxacillin is similar to penicillin. It binds to the main penicillin-binding proteins (PBPs) on the bacterial cell wall, affecting the synthesis of the bacterial cell wall, causing the bacteria to swell, burst and die, thus playing an antibact...

AI Technical Summary

Problems solved by technology

[0007] The first method uses ultra-high performance liquid chromatography-tandem mass spectrometry. This method is difficult to operate, the processing steps are cumbersome, time-consuming, and the cost of the instrument is extremely high. It is difficult for ordinary units or enterprises to afford, and the quality requirements for operators High, the overall detection cost is too high

The second method uses ordinary liquid chromatography. Although the operation is simpler than the first method, the use of chloroform as the stripping reagent is more harmful, and the precipitation and degreasing operations in the process may have large errors, resulting in partial recovery. Low, more false positives are often caused in the actual operation process, and the method is mainly aimed at the milk matrix, whether it is also applicable to complex matrices such as meat is also open to question

The above methods have high costs for instruments, reagents, labor, etc., and are not suitable for large-scale implementation, and are not suitable for the increasing detection needs.

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