Anti-tumor drug delivery system and preparation method and application thereof
A technology for anti-tumor drugs and delivery systems, applied in anti-tumor drugs, pharmaceutical formulations, drug combinations, etc., can solve the problems of high preparation costs and weak immune efficacy, achieve high drug loading, reduce phagocytosis, and enhance the immune function of the body Effect
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Embodiment 1
[0082] Example 1 Preparation of antineoplastic drug delivery system
[0083] In this example, the red blood cell membrane was loaded with irinotecan hydrochloride to obtain the drug-loaded red blood cells, and the obtained drug-loaded red blood cells were labeled as CPT-11-RBCs, and the drug-loaded red blood cells (CPT-11-RBCs) were mixed with the alumina solution, The antineoplastic drug delivery system was prepared and marked as AN-CPT-11-RBC. Including the following steps:
[0084] (1) Preparation of carrier red blood cells
[0085] Take about 200g of SPF male SD rats, use a melting point capillary (inner diameter × tube length = (0.9-1.1) × 100mm) to collect blood from the retro-orbital venous plexus of the rats, and take about 0.2-0.4mL each time to infiltrate In a centrifuge tube with sodium heparin, centrifuge at 2000r / min, 4°C for 4min, discard the upper layer of colorless and transparent plasma and white blood cell layer, add 500μL of pre-cooled PBS solution, wash 2...
Embodiment 2
[0095] The mensuration of embodiment 2 drug loads
[0096] In this example, the effects of treatment time of the hypotonic solution and different concentrations of irinotecan hydrochloride on the drug loading were investigated. Including the following steps:
[0097] (1) Collect the drug-containing supernatant during the drug-loading process
[0098] Using 0.6 wt% sodium chloride aqueous solution as the hypotonic solution, red blood cells were pretreated at 0°C for 5 min, 10 min, 20 min, and 30 min, and the rest of the steps were the same as in Example 1 to prepare an antineoplastic drug delivery system. During the preparation process of each treatment, the supernatant A in step (3) in Example 1 is mixed with the supernatant B in step (4) to obtain a mixed supernatant, and then draw 0.2 mL Add 0.8 mL of methanol to the mixed supernatant, vortex mix, centrifuge at 12000 rpm / min and 4°C for 10 min to obtain a mixed solution, and then detect the mixed solution by high performan...
Embodiment 3
[0120] Example 3 Determination of the adsorption capacity of nano-alumina in the antineoplastic drug delivery system
[0121] In this example, the influence of different concentrations of alumina nano-solutions on the adsorption amount of nano-alumina in the antineoplastic drug delivery system (AN-CPT-11-RBCs) was investigated. Including the following steps:
[0122] (1) Preparation of calcein-labeled nano-alumina
[0123] Weigh a certain amount of calcein (Calcein) powder and nano-alumina powder in a beaker with a mass ratio of 1:10, add a certain amount of deionized water, mix and stir on a magnetic stirrer for 45 minutes, and set the temperature at 1600rpm / min and Centrifuge at 4°C for 5 min. Add deionized water to wash 2-3 times until the supernatant after centrifugation is nearly colorless, add an appropriate amount of PBS solution to resuspend the calcein-labeled nano-alumina, and ultrasonically disperse for 30 minutes to obtain calcein-labeled nano-alumina solution (...
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