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Method for simultaneously and quantitatively detecting taurine and methyl taurine in sodium lauroyl methyl taurate by utilizing high-performance liquid-phase ultraviolet method

A technology of sodium lauroyl methyl taurine and methyl taurine, which is applied in the detection field, can solve the problems of inability to meet the requirements, high requirements for instrumental personnel, difficult operation, etc., and achieves conditions that are easy to meet and have good accuracy. , the effect of low operating requirements

Pending Publication Date: 2022-04-22
广州星业科技股份有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The chemical method includes the nitrogen determination method, which is mainly used in the detection of acid-containing preparations to overcome the inaccurate titration caused by the increase in the amount of alkaline substances; the instrument method uses more methods, including differential refraction detectors and ELSD detection instrument detection, high performance liquid chromatography tandem mass spectrometry detection, ion chromatography detection and magnetic resonance nuclear spin singlet selective detection. sour request

Method used

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  • Method for simultaneously and quantitatively detecting taurine and methyl taurine in sodium lauroyl methyl taurate by utilizing high-performance liquid-phase ultraviolet method
  • Method for simultaneously and quantitatively detecting taurine and methyl taurine in sodium lauroyl methyl taurate by utilizing high-performance liquid-phase ultraviolet method
  • Method for simultaneously and quantitatively detecting taurine and methyl taurine in sodium lauroyl methyl taurate by utilizing high-performance liquid-phase ultraviolet method

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Experimental program
Comparison scheme
Effect test

Embodiment 1-10

[0035] The present inventors found that the derivation environment temperature in the pretreatment process of sodium lauroyl methyl taurine is closely related to the separation degree R between methyl taurine and taurine. Then set the gradient water bath temperature to detect the separation degree of methyl taurine and taurine, specifically:

[0036] Take 10 50ml volumetric flasks, weigh 10 parts of sodium lauroyl methyl taurate 0.2g (accurate to 0.01mg) and place them in the volumetric flasks respectively, make up to 10ml with distilled water, and add 1ml of carbonic acid with a mass concentration of 5%. Sodium, add 1.5ml of 4-benzyl bromide with a mass concentration of 1% dropwise, shake the bottle body while dropping to make the reaction fully, and place at 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C , 70°C, 75°C, and 80°C in a water bath for 1 hour. After the reaction, take out the volumetric flask, add 0.5ml of phosphoric acid with a mass concentration of 10% to terminate th...

Embodiment 11-13

[0053] Embodiment 11-13: Accuracy experiment

[0054] Accurately weigh 3 parts of sodium lauroyl methyl taurate, the weight is shown in Table 4 (accurate to 0.01mg), respectively placed in a 50ml volumetric flask, and the stock solution (1000mg / mL) prepared in Comparative Examples 1-5 was taken as Reference substance (known concentration), add 1mL, 2ml, 4ml respectively, make up to 10ml with distilled water, add 1ml of sodium carbonate with a mass concentration of 5%, add dropwise 1.5ml of 4-nitrobenzyl bromide with a mass concentration of 1%, Shake the bottle body while dripping to make it fully react, and place it in a water bath at 55°C for 1 hour. After the reaction is over, take out the volumetric flask, add 0.5ml of phosphoric acid with a mass concentration of 10% to stop the reaction, and make up to the mark with distilled water line, sonicate for 1 min to remove air bubbles. Filter through a syringe filter into a vial. Gained sample injection is carried out ultraviol...

Embodiment 14-19

[0067] Embodiment 14-19: Precision experiment

[0068] Take the sodium lauroyl methyl taurate solution of the above comparative example 6, inject the sample 6 times continuously, and record the precision of the peak area of ​​the main component as shown in Table 5.

[0069] Table 5 precision test results

[0070]

[0071] Table 5 result shows: detection method precision of the present invention is good

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Abstract

The invention belongs to the field of detection, and discloses a method for simultaneously and quantitatively detecting taurine and methyl taurine in sodium lauroyl methyl taurate by using a high performance liquid chromatography ultraviolet method. The method comprises the following steps: preparing a series of standard substance samples by using methyl taurine and taurine standard substances; performing ultraviolet spectrum detection on the standard sample to obtain peak areas of taurine and methyl taurine; respectively taking methyl taurine and taurine concentrations as horizontal coordinates and respective peak areas as vertical coordinates to obtain two linear equations; preparing a to-be-detected sample from sodium lauroyl methyl taurate; performing ultraviolet spectrum detection on the to-be-detected sample to obtain peak areas of taurine and methyl taurine; respectively substituting into a linear equation, and calculating to obtain the concentration of the methyl taurine and the concentration of the taurine with good linearity within the concentration range of 0-10mg / ml (rgt; 0.999) of the method; the accuracy and the repeatability are good; and the solution is stable after being placed for 24 hours and 48 hours.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a method for quantitatively detecting taurine and methyl taurine in sodium lauroyl methyl taurate simultaneously by using a high-performance liquid phase ultraviolet method. Background technique [0002] Taurine and methyl taurine are both easily soluble in water and stable in nature. They are commonly found in the organs of humans and mammals. They are one of the most important amino acids. They have special pharmacological and physiological functions and can reduce inflammation. , analgesic, antipyretic, hypoglycemic, maintain normal visual function, taurine also has the ability to enhance cell anti-oxidation, anti-free radical damage and anti-viral damage, and plays an important role in improving immunity. [0003] Sodium lauroyl methyl taurate has a sulfonic acid group, has good compatibility with anionic, nonionic and amphoteric surfactants, is easily soluble in water, has ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74G01N2030/027G01N2030/067
Inventor 谢佩佩孟巨光李建许林寿
Owner 广州星业科技股份有限公司
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