Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Micro-nano double-layer structure antibacterial stent as well as preparation method and application thereof

A double-layer structure, micro-nano technology, applied in the direction of antibacterial drugs, nanotechnology, nanotechnology, etc., can solve the problems of bacterial infection, low biocompatibility and low mechanical strength of polymer membranes, and achieve good antibacterial properties, Good cell compatibility, the effect of increasing the range of use

Active Publication Date: 2022-05-03
PEKING UNIV SCHOOL OF STOMATOLOGY
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a micro-nano double-layer structure antibacterial scaffold and its preparation method and application, which solves the problems of easy bacterial infection, low mechanical strength, low biocompatibility of polymer film and easy Cause rejection of the body, poor stability of traditional antibacterial drugs, low oral bioavailability, poor drug targeting technical problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro-nano double-layer structure antibacterial stent as well as preparation method and application thereof
  • Micro-nano double-layer structure antibacterial stent as well as preparation method and application thereof
  • Micro-nano double-layer structure antibacterial stent as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] The invention provides a method for preparing an antimicrobial bracket with a micro-nano double-layer structure, comprising the following steps:

[0030] (1) polyhydroxyalkanoate material is mixed with nanometer antibacterial drug, add organic solvent to dissolve, obtain polyhydroxyalkanoate solution;

[0031] (2) Pour the polyhydroxyalkanoate solution into the mold, immerse the mold containing the polyhydroxyalkanoate solution in the poor solvent of the organic solvent, and perform solution replacement to obtain a semi-solid stent;

[0032] (3) After the semi-solid stent is freeze-dried, an antibacterial stent with a micro-nano double-layer structure is obtained.

[0033] In the invention, the polyhydroxyalkanoate material is detoxified and purified before preparing the antimicrobial bracket with a micro-nano double-layer structure.

[0034] In the present invention, the detoxification preferably uses a Soxhlet extractor to extract and remove endotoxins in polyhydroxy...

Embodiment 1

[0075] The PHA material used in this example is poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB), which was purchased from Beijing Weimogongchang Biotechnology Co., Ltd. The nano-antibacterial drug used is nano-zinc oxide particles (diameter between 40nm and 100nm), purchased from Beijing Zhongjinyan New Material Technology Co., Ltd.

[0076] 50g of P34HB raw material was extracted by Soxhlet extractor to remove endotoxin. The extraction process is as follows: (1) Turn on the circulating water switch; (2) Set the program: use chloroform to extract and rinse at a temperature of 140°C, and the time is 3h and 2h respectively. The temperature was lowered to 40° C., the extraction cylinder was taken out, and the solvent was completely evaporated to obtain 30 g of crude extraction material of P34HB, with a loss rate of 40%. Add 300ml of chloroform to 30g of P34HB crude extraction material, spin to dissolve, and filter the solution through double-layer gauze to filter out insolu...

Embodiment 2

[0081] Cell Proliferation Experiment of Human Gingival Fibroblasts on Different Scaffolds

[0082] In a sterile environment, the P34HB+0.5% ZnO antibacterial support prepared in Example 1 was cut into a suitable circle according to the pore size of the 96-well plate and placed in the 96-well plate. When the antibacterial support was placed, the micro-pore structure layer was facing upwards Contact with cells, then inoculate human gingival fibroblasts, 5000 cells / well, in DMEM medium containing 10% fetal bovine serum (Gibco, USA) and 1% penicillin / streptomycin (Gibco, USA) at 37°C and 5%CO 2 Incubate in the incubator for 1, 4, 7 days. And with the P34HB material purified in Example 1 as a blank, the antibacterial scaffold (P34HB+1%ZnO) with a HealAll collagen film (Zhenghai Biology, China) and a nano-zinc oxide concentration of 1% prepared according to the method in Example 1 was used as a contrast ,conduct experiment. Three replicate wells were set up for each treatment and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Apertureaaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a micro-nano double-layer structure antibacterial stent as well as a preparation method and application thereof, and belongs to the technical field of oral medical products. The preparation method of the micro-nano double-layer structure antibacterial stent provided by the invention comprises the following steps: (1) mixing a polyhydroxyalkanoate material with a nano antibacterial drug, and adding an organic solvent for dissolving to obtain a polyhydroxyalkanoate solution; (2) pouring a polyhydroxyalkanoate solution into a mold, immersing the mold filled with the polyhydroxyalkanoate solution into the poor solvent of the polyhydroxyalkanoate material, and carrying out solution replacement to obtain a semisolid stent; and (3) freeze-drying the semi-solid scaffold to obtain the micro-nano double-layer structure antibacterial scaffold. The micro-nano double-layer structure antibacterial scaffold provided by the invention has good cytocompatibility and antibacterial property, and can effectively promote adhesion and proliferation of gingival fibroblasts and reduce bacterial infection of wounds.

Description

technical field [0001] The invention relates to the technical field of oral medical products, in particular to a micro-nano double-layer structure antibacterial bracket and its preparation method and application. Background technique [0002] Soft tissue augmentation is a standard procedure prior to implant surgery and prosthetic restorations, and was achieved by Friedman in 1957 in the membranous gingival procedure. This procedure maintains sufficient keratinized or attached gingival tissue to prevent further loss of gingival attachment. Autologous tissue is the gold standard for traditional soft tissue augmentation procedures in terms of gingival width, thickness, aesthetics, and long-term stability. However, the use of autologous tissue needs to open up a second operation area, the amount of soft tissue available in the donor area is relatively limited, the graft valve is prone to contraction and does not match the recipient area, and the incidence of complications (such...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/40A61L27/18A61L27/04A61L27/02A61L27/50A61L27/54A61L27/56A61K47/34A61K45/00A61P1/02A61P31/04B82Y5/00B82Y40/00
CPCA61L27/18A61L27/047A61L27/025A61L27/045A61L27/50A61L27/54A61L27/56A61K47/34A61K45/00A61P1/02A61P31/04B82Y5/00B82Y40/00A61L2430/34A61L2300/404A61L2400/12A61L2300/104A61L2300/102A61L2300/10C08L67/04
Inventor 孙玉春陈国强张旭周永胜王勇陈凡凡赵梓帆翟文茹
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com