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Lactobacillus plantarum DMDL9010 microcapsule as well as preparation method and application thereof

A technology of Lactobacillus plantarum and microcapsules, applied in the field of food processing, can solve the problems of large particle size of microcapsules, complicated preparation methods, poor embedding and survival effects, etc., and achieves uniform particles, good dispersibility, and strong storage stability. sexual effect

Pending Publication Date: 2022-05-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the deficiencies in the technology or process of preparing microcapsules by the existing emulsification method, the preparation method is complicated, the particle size of the microcapsules is large, and the embedding and survival effects are not good. The purpose of the present invention is to provide a preparation of Lactobacillus plantarum DMDL 9010 microcapsules Method and its application

Method used

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  • Lactobacillus plantarum DMDL9010 microcapsule as well as preparation method and application thereof
  • Lactobacillus plantarum DMDL9010 microcapsule as well as preparation method and application thereof
  • Lactobacillus plantarum DMDL9010 microcapsule as well as preparation method and application thereof

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Effect test

Embodiment 1

[0047] Embodiment 1: A kind of preparation method of Lactobacillus plantarum DMDL 9010 microcapsules

[0048] (1) Activation of strains: Inoculate the DMDL 9010 bacterial solution stored in the glycerol tube into the liquid medium (inoculum size 2%, v / v), and incubate in a biochemical incubator at a constant temperature of 37°C for 16 hours. The activated Lactobacillus plantarum DMDL 9010 is used as the seed liquid, and the bacterial content of the seed liquid is about 10 8 CFU / mL;

[0049] (2) Expanded cultivation: the above-mentioned seed bacteria solution was inserted into the expanded fermentation medium with an initial pH of 6.0 at a volume ratio of 2:100, cultured on a shaking table, the fermentation temperature was 32°C, the inoculum size was 9%, and fermented for 28 hours to obtain plant milk Bacillus DMDL 9010 fermentation broth;

[0050] (3) Pretreatment: Centrifuge the fermentation broth of Lactobacillus plantarum DMDL 9010, discard the supernatant, take the preci...

Embodiment 2

[0056] Embodiment 2: A kind of preparation method of Lactobacillus plantarum DMDL 9010 microcapsules

[0057] (1) Activation of strains: Inoculate the DMDL 9010 bacterial solution stored in the glycerol tube into the liquid medium (inoculum size 2%, v / v), and incubate in a biochemical incubator at a constant temperature of 37°C for 16 hours. The activated Lactobacillus plantarum DMDL 9010 is used as the seed liquid, and the bacterial content of the seed liquid is about 10 8 CFU / mL;

[0058] (2) Expanded cultivation: the above-mentioned seed bacteria solution was inserted into the expanded fermentation medium with an initial pH of 6.0 at a volume ratio of 2:100, cultured on a shaking table, the fermentation temperature was 32°C, the inoculum size was 9%, and fermented for 28 hours to obtain plant milk Bacillus DMDL 9010 fermentation broth;

[0059] (3) Pretreatment: Centrifuge the fermentation broth of Lactobacillus plantarum DMDL 9010, discard the supernatant, take the preci...

Embodiment 3

[0065] Embodiment 3: A kind of preparation method of Lactobacillus plantarum DMDL 9010 microcapsules

[0066] (1) Activation of strains: Inoculate the DMDL 9010 bacterial solution stored in the glycerol tube into the liquid medium (inoculum size 2%, v / v), and incubate in a biochemical incubator at a constant temperature of 37°C for 16 hours. The activated Lactobacillus plantarum DMDL 9010 is used as the seed liquid, and the bacterial content of the seed liquid is about 108CFU / mL;

[0067] (2) Expanded cultivation: the above-mentioned seed bacteria solution was inserted into the expanded fermentation medium with an initial pH of 6.0 at a volume ratio of 2:100, cultured on a shaking table, the fermentation temperature was 32°C, the inoculum size was 9%, and fermented for 28 hours to obtain plant milk Bacillus DMDL 9010 fermentation broth;

[0068] (3) Pretreatment: Centrifuge the fermentation broth of Lactobacillus plantarum DMDL 9010, discard the supernatant, take the precipit...

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Abstract

The invention belongs to the field of food processing, and discloses a lactobacillus plantarum DMDL 9010 microcapsule as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving chitosan in a lactic acid solution, adjusting the pH value to 3-5, adding lactobacillus plantarum DMDL 9010 activated bacterial sludge, fully stirring, then adding a genipin solution, uniformly mixing, then adding plant oil containing span 80, uniformly mixing, stirring to prepare a water-in-oil emulsion, carrying out a cross-linking reaction to obtain a microcapsule suspension, washing with ethyl acetate, and drying to obtain the chitosan microcapsule. And drying to obtain the lactobacillus plantarum DMDL 9010 microcapsule freeze-dried powder. The obtained lactobacillus plantarum DMDL 9010 microcapsule has the advantages of high embedding rate, small particle size, high gastrointestinal tract tolerance, simple preparation method and the like, and has relatively high application value.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a microcapsule of Lactobacillus plantarum DMDL 9010 and a preparation method and application thereof. Background technique [0002] Lactobacillus plantarum belongs to the Bacillus class Lactobacillus genus probiotics, is an anaerobic or facultative anaerobic Gram-positive bacilli, usually found in fermented vegetables, fruit juices, fermented dairy products and meat, with rich health benefits Effects, such as enhancing the body's immunity, maintaining the balance of intestinal flora, reducing serum cholesterol levels, alleviating lactose intolerance and inhibiting the formation of tumor cells, etc. [0003] According to data from the Food and Agriculture Organization (FAO) and the World Health Organization (WHO), the minimum number of bacteria required for probiotics to exert a beneficial effect is 10 6 -10 7 CFU / mL. Probiotics must pass through the digestive system ...

Claims

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Application Information

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IPC IPC(8): A23L33/135A23L29/30A23L29/00A23P10/30A23P10/35C12N1/20C12R1/25
CPCA23L33/135A23L29/30A23L29/035A23P10/30A23P10/35C12N1/20A23V2002/00A23V2400/169A23V2200/30A23V2250/511
Inventor 刘冬梅熊杰黄燕燕肖弘毅
Owner SOUTH CHINA UNIV OF TECH
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