Microbial agent for preventing both root knot nematode disease and clubroot and preparation method thereof

A microbial agent and technology of clubworm, applied in the field of fertilizers, can solve the problems of difficulty in timely prevention and control, difficulty in quickly distinguishing root-knot nematode disease from clubroot disease, and simultaneous occurrence of root-knot nematode disease and clubroot disease. Promote growth, improve crop resistance, and improve the effect of absorption and utilization

Pending Publication Date: 2022-05-17
YUNNAN YUNYE FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to propose a microbial agent for preventing root-knot nematode and clubroot and its preparation method, which solves the problem of similar field symptoms of root-knot nematode and clubroot in the prior art, which is difficult to quickly distinguish and accurately select Chemicals, root-knot nematode and clubroot are prone to occur simultaneously and are difficult to prevent and control in time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The present embodiment provides a kind of microbial inoculum for preventing root-knot nematode and clubroot, and the specific preparation method is as follows:

[0041] The first step: preparing methylotrophic bacillus bacteria powder and bacillus subtilis bacteria powder

[0042] The methylotrophic Bacillus and Bacillus subtilis preserved in glycerol were taken out from -80°C, inoculated into liquid LB medium at a ratio of 3‰, and cultured with shaking at 38°C for 24 hours to obtain the strains of the two strains. seed liquid. The activated seed liquid is inoculated into the first-level medium according to the ratio of 10% for cultivation to obtain the first-level seed liquid, and then according to the proportion of 60% of the volume, the first-level seed liquid is respectively inoculated into the second-level medium for cultivation, Obtain secondary seed solution. Next, the secondary seed liquids were respectively introduced into disc centrifuges, centrifuged at a r...

Embodiment 2

[0056] The present embodiment provides a kind of microbial inoculum for preventing root-knot nematode and clubroot, and the specific preparation method is as follows:

[0057] The first step: preparing methylotrophic bacillus bacteria powder and bacillus subtilis bacteria powder.

[0058]The methylotrophic Bacillus and Bacillus subtilis preserved in glycerol were taken out from the condition of -80°C, respectively inoculated into the liquid LB medium according to the ratio of 5‰, and the two strains were obtained after shaking culture at 35°C for 48 hours. seed liquid. The activated seed liquid is inoculated into the first-level medium according to the proportion of 5% for cultivation to obtain the first-level seed liquid, and then the first-level seed liquid is respectively inoculated into the second-level medium according to the proportion of 70% by volume for cultivation, Obtain secondary seed solution. Next, the secondary seed liquids were respectively introduced into di...

Embodiment 3

[0072] The present embodiment provides a kind of microbial inoculum for preventing root-knot nematode and clubroot, and the specific preparation method is as follows:

[0073] The first step: preparing methylotrophic bacillus bacteria powder and bacillus subtilis bacteria powder.

[0074] The methylotrophic Bacillus and Bacillus subtilis preserved in glycerol were taken out from -80℃, inoculated into liquid LB medium at a ratio of 4‰, and cultured with shaking at 37℃ for 35 hours to obtain the two strains. seed liquid. The activated seed liquid is inoculated into the primary medium according to the ratio of 8% for cultivation to obtain the primary seed liquid, and then the primary seed liquid is respectively inoculated into the secondary medium according to the volume of 65% for cultivation, Obtain secondary seed solution. Next, the secondary seed liquids were respectively introduced into disc centrifuges, centrifuged at a rotational speed of 6000r / min for 15min, and the sup...

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Abstract

The invention discloses a microbial agent capable of preventing both root knot nematode disease and clubroot and a preparation method of the microbial agent, and relates to the technical field of fertilizers. The technical problems that in the prior art, field symptoms of the root-knot nematode disease and the clubroot disease are similar, rapid distinguishing and accurate medicament selection are difficult, and the root-knot nematode disease and the clubroot disease easily occur at the same time and are difficult to prevent and control in time are solved. The preparation method of the microbial agent for preventing both the root knot nematode disease and the clubroot disease comprises the following steps: preparing bacillus methylotrophicus powder and bacillus subtilis powder; preparing paecilomyces lilacinus powder; and compounding the prepared bacillus methylotrophicus powder, paecilomyces lilacinus powder and bacillus subtilis powder in proportion, and uniformly mixing to obtain the microbial agent capable of preventing both the root knot nematode disease and the clubroot disease. The prepared microbial agent for simultaneously preventing the root knot nematode disease and the clubroot disease can be used for uniformly preventing and controlling the two diseases, and the situation that the golden period of early prevention and control is delayed due to wrong medicament selection in agricultural production is avoided.

Description

technical field [0001] The invention relates to the technical field of fertilizers, in particular to a microbial agent for preventing root-knot nematode and clubroot and a preparation method thereof. Background technique [0002] Clubroot is caused by Plasmodium brassicae, a fungal disease that only occurs in cruciferous vegetables, such as Chinese cabbage, pak choy, cabbage, radish, etc. After the onset of clubroot, the main root and lateral root of the plant swell to form tumors or chicken intestinal roots of different sizes. The shape and size are greatly affected by the planting site. The tumors on the main root are mostly close to the upper part, spherical or nearly spherical, and the surface is uneven. , Rough, and sometimes the epidermis cracks in the later stage; the tumors on the lateral roots are mostly cylindrical or finger-shaped. There are also some plants that after the onset of the disease, the upper part of the main root is stimulated by the pathogen, and ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/30A01P3/00A01P5/00A01P21/00C12R1/07C12R1/125C12R1/79
CPCC12N1/20C12N1/14A01N63/22A01N63/30
Inventor 盘文政赵学通黄晓杰王斌徐思琪华小兵朱永立李建云李荣海笪颖飞
Owner YUNNAN YUNYE FERTILIZER
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