Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting and purifying procyanidine from peanut red skin

A purification method, the technology of the original flower of red coat, applied in the direction of organic chemistry, bulk chemical production, etc., can solve the problems of long extraction time, high cost, low extraction rate, etc., to achieve improved extraction rate, high innovation, and improved extraction efficiency effect

Pending Publication Date: 2022-05-24
HENAN AGRICULTURAL UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent extraction method takes a long time to extract, and the extraction rate is generally not high; the supercritical extraction method has higher yield and better selectivity than the traditional assisted organic solvent extraction method, but the cost is high; microwave-assisted extraction method and ultrasonic-assisted extraction method have shortening Extraction time, reduce the amount of extractant, increase the yield, green and environmental protection, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting and purifying procyanidine from peanut red skin
  • Method for extracting and purifying procyanidine from peanut red skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The present embodiment provides a method of extraction and purification of arachidon proanthocyanidins, comprising the following steps:

[0040] Step 1, after drying the peanut red coat, crush it to a particle size of 60 mesh, obtain the peanut red coat powder, and then soak the peanut red coat powder in the petroleum ether for 12 h, reduce the pressure to remove the petroleum ether, and then dry it to a water content of 7%, to obtain the degreased peanut red coat.

[0041] Step 2, take 1g of degreased peanut red coat powder in a beaker, add 40mL 70% ethanol solution to mix evenly and then place it in a microwave light wave ultrasonic extractor for ultrasonic microwave treatment, seal the beaker and then dip it in a 50 °C water bath pot for 20min, and then decompress the obtained product, and use a sterile membrane with a pore diameter of 450 μm to carry out membrane filtration of the filtrate after decompression extraction, and the filtrate obtained by membrane filtration ...

Embodiment 2

[0049] The present embodiment provides a method of extraction and purification of arachidon proanthocyanidins, comprising the following steps:

[0050] Step 1, after drying the peanut red coat, crush it to a particle size of 50 mesh, obtain the peanut red coat powder, then soak the peanut red coat powder in petroleum ether for 10 h, reduce pressure to filter out the petroleum ether, and then dry it to a water content of 6%, to obtain the degreased peanut red coat.

[0051] Step 2, take 1g of degreased peanut red coat powder in a beaker, add 50mL 60% ethanol solution to mix evenly and then place it in a microwave light wave ultrasonic extractor for ultrasonic microwave treatment, seal the beaker and then immerse it in a 40 °C water bath pot for 40 min, and then decompress the obtained products, and use a sterile membrane with a pore diameter of 450 μm to carry out membrane filtration treatment of the filtrate after decompression extraction, and the filtrate obtained by membrane fil...

Embodiment 3

[0059] The present embodiment provides a method of extraction and purification of arachidon proanthocyanidins, comprising the following steps:

[0060] Step 1, the peanut red coat is dried and crushed to a particle size of 70 mesh, the peanut red coat powder is obtained, and then the peanut red coat powder is soaked in petroleum ether for 14 h, and the petroleum ether is removed by vacuum extraction, and then dried to a water content of 8%, and the peanut red coat after defatting is obtained.

[0061]Step 2, take 1 g of degreased peanut red coat powder in a beaker, add 50mL of 80% ethanol solution to mix evenly and then placed in a microwave light wave ultrasonic extractor for ultrasonic microwave treatment, seal the beaker and then immerse it in a 60 °C water bath pot for 10 min, then decompress the obtained product, and use a sterile membrane with a pore diameter of 450 μm to carry out membrane filtration of the filtrate after decompression extraction, and the filtrate obtained ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of peanut red skin effective active ingredient extraction, and discloses a peanut red skin procyanidine extraction and purification method, which comprises: drying peanut red skin until the water content is 6-8%, crushing until the particle size is 50-70 mesh, soaking in petroleum ether for 10-14 h, and drying to obtain degreased peanut red skin; the preparation method comprises the following steps: uniformly mixing degreased peanut skin with an alcohol solution, then carrying out ultrasonic-microwave synergistic treatment, then extracting for 10-40 minutes at the temperature of 40-60 DEG C, and carrying out solid-liquid separation to obtain a first extracting solution; diluting the first extracting solution by 3-5 times, carrying out column chromatography purification treatment through a chromatographic column, and carrying out vacuum concentration and freeze drying to obtain purified peanut red skin procyanidine powder. The method combines an ultrasonic extraction method and a microwave extraction method, and has the advantages of improving the efficiency, greatly shortening the extraction time, reducing the energy consumption and the like.

Description

Technical field [0001] The present invention relates to the field of peanut red coat active ingredient extraction technology, in particular to a peanut red coat proanthocyanidin extraction purification method. Background [0002] Peanut skin (PS), a legume peanut seed coat, is usually pink or red in color and bitter in taste. As a by-product of peanut deep processing, the global output is about 930,000 tons / year, mostly as waste, but also as animal feed for animal husbandry, the utilization and added value of products are low, resulting in great waste and pollution of resources. Peanut red coat is rich in active ingredients, such as tannic acid, resveratrol, procyanidins (PSPc), etc., especially PSPc content of up to 128g / kg, is a kind of polyphenolic substances that are commonly present in plants. Studies have shown that PSPc has antioxidant, bacteriostatic, anti-cancer, hypolipidemic, improved blood glucose levels, anti-allergy and inhibition of acrylamide production of biolo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D311/62
CPCC07D311/62Y02P20/54
Inventor 王娜余秋颖任红涛王凡徐超李春英崔晨旭宁灿灿郑玉茹李正邦
Owner HENAN AGRICULTURAL UNIVERSITY