Acremonium persicae H1-1 as well as screening method and application thereof
Acremonium anemone, metabolite technology, applied in the direction of application, microbe-based methods, botanical equipment and methods, etc., to achieve the effect of increasing survival rate
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[0042] Based on the same inventive concept, the present application also provides a preparation method for an inhibitor of sclerotiorum, comprising the following steps:
[0043] Inoculate the above-mentioned Acremonium peach color H1-1 into PDB medium, shake and culture at 150-200 r / min for 5-10 d, filter, and collect the supernatant by centrifugation to obtain the inhibitor of S. chinensis.
[0044] Specifically, use a sterile hole puncher (5mm in diameter) to punch holes at the edge of the colony of Acremonium peach, pick 3 pieces of bacteria and inoculate them into a 100ml Erlenmeyer flask containing 50ml PDB medium, at 180r / min , 28 ℃ constant temperature shaking culture for 7d until the culture solution is pink, filter the culture solution with three layers of lens paper, centrifuge the filtered culture solution at 8000r / min for 15min, and collect the supernatant to obtain the inhibition of S. agent. When the mass concentration of the inhibitor reaches 20%, the growth of...
Embodiment 1
[0050] The examples of the present application provide an Acremonium peach color H1-1, and the Acremonium peach color H1-1 preservation number is CCTCCNO: M2022055.
[0051] The above-mentioned screening method of Acremonium peach H1-1, comprising the following steps:
[0052] Weigh 2g of the sediment mixture collected from the ocean beach, add it to a triangular flask containing 18mL of sterile water and glass beads, shake and mix with a vortex shaker to make a suspension, and dilute it sequentially with a 10-fold gradient dilution method. From a dilution factor of 10 -2 , 10 -3 , 10 -4 and 10 -5 Pipette 100 μL of the suspension from the prepared spore and spread it on the PDA medium containing 0.1 mol / L NaCl, invert at 28 °C, and pick a single colony with different shapes when the colony is produced.
[0053] The preparation method of PDA medium is as follows: take 200g of potatoes and boil in purified water for 10min, filter with 4 layers of gauze to remove potato piece...
Embodiment 2
[0055] Strain identification
[0056] The bacterial strain H1-1 isolated in Example 1 was tapetum-shaped on the PDA plate, the mycelium was not easy to be lifted from the culture substrate, the colony was white at the initial stage, and pale pink at the later stage, and the surface of the mycelium was filled with conidia, The conidia were observed to be oval or round under a 40x microscope, and they could still grow normally under NaCl treatment. Specifically, the colony morphology and spore morphology of the strains isolated in Example 1 are as follows: figure 1 shown. The bacterial colony morphology of the bacterial strain isolated in Example 1 under NaCl treatment is as follows: figure 2 As shown, Figure 2 shows the colony morphology of the strain under 0.1 mol / L NaCl treatment, and the colony morphology without NaCl treatment (that is, corresponding to 0 mol / L NaCl). The DNA of the strain H1-1 isolated in Example 1 was subjected to PCR amplification to obtain the ITS r...
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