Polypeptide with anti-wrinkle function and compound and application thereof
A complex and functional technology, applied in the field of bioengineering, can solve problems such as unsatisfactory stability and affinity penetration of the skin barrier, adverse reactions, breathing difficulties, etc., and achieve good biocompatibility, good permeability and affinity, The effect of eliminating or reducing wrinkles
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Embodiment 1
[0037] This embodiment provides the preparation method of the polypeptide with anti-wrinkle function.
[0038] Using total chemical synthesis of peptide technology, using Boc chemical solid-phase peptide synthesis (SPPS) developed by Kent and colleagues, HBTU / HOBt-based Fmoc method optimized HBTU activation / DIEA in situ neutralization scheme provided by ABI, in CSbio All peptides were synthesized on MBHA resin in a 336X automated peptide synthesizer. In HF (Boc Chemicals) or with 88% TFA, 5% phenol, 5% H 2 After cleavage and deprotection in a reagent mixture of O and 2% TIPS (Fmoc chemistry), the crude product was precipitated with cold diethyl ether and purified to homogeneity by preparative C18 reverse phase HPLC method.
[0039] The obtained polypeptide with anti-wrinkle function was prepared into a 1 mg / mL solution, and the molecular weight was determined by electrospray ionization mass spectrometry (ESI-MS). And its purity is detected by high performance liquid chromato...
Embodiment 2
[0041] This embodiment provides a process for preparing a complex from the polypeptide with anti-wrinkle function.
[0042] 4.5mL pure water, 0.5mL HAuCl 4 (10mM), 0.4mL vitamin C ascorbic acid (0.2M) were added into the beaker in sequence, and stirred at 650rpm until it turned into a wine red solution. Weigh 5.4 mg of the polypeptide with anti-wrinkle function, dissolve it in 21.6 mL of pure water, and add it to the wine-red solution after it is completely dissolved by ultrasonic waves, and stir to form the complex.
[0043] The obtained complex is detected by high performance liquid chromatography, and the test results are as follows: figure 2 shown. Depend on figure 2 It can be seen that the molecular weight of the polypeptide in the complex is 1947Da, which further verifies that the sequence of the peptide is correct; the peak emerges at 13.61 min, and a single chromatographic peak appears, and the analytical HPLC identifies the peptide in the complex. The purity of th...
Embodiment 3
[0045] This embodiment provides a process for preparing a complex from the polypeptide with anti-wrinkle function.
[0046] 4.5mL pure water, 0.5mL HAuCl 4 (10mM), 0.4mL vitamin C ascorbic acid (0.6M) were added to the beaker in turn, and stirred at 650rpm until it turned into a wine red solution. Weigh 5.4 mg of the polypeptide with anti-wrinkle function, dissolve it in 21.6 mL of pure water, and add it to the wine-red solution after it is completely dissolved by ultrasonic waves, and stir to form the complex.
[0047] Draw 10 μL of the obtained complex and drop it on the carbon support film. After the carbon support film is dry, measure its shape and size by transmission electron microscope. The test results are as follows: image 3 shown.
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Abstract
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