Moringa oleifera straw mediated synthesized biological tellurium nanoparticles and antibacterial and antiviral application thereof
A technology of synthetic biology and tellurium nanometers, applied in antiviral agents, antibacterial drugs, and resistance to vector-borne diseases, etc., can solve the problems of no reports, etc., and achieve the effect of high antibacterial activity, low source, and high stability
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Embodiment 1
[0034] Example 1 Synthesis of biological tellurium nanoparticles
[0035] (1) Mix 100g of Moringa stalks (stalks, leaves) with 1000mL of water, and use a 2100W induction cooker at 240°C to stir and heat for 10min;
[0036] (2) The solid-liquid mixture was filtered with 8 layers of gauze, and the crude extract was filtered twice with a water circulating vacuum pump. Finally, 600 mL of filtrate was collected, and the filtrate was divided into 250 mL conical flasks (100 mL / bottle), placed in 600 mL conical flasks. ℃, save for 16h;
[0037] (3) In a 100 mL glass beaker, mix 50 mL of the Moringa oleifera straw extract, which was stored at 6 °C for 16 h, with 0.2 g of potassium tellurite, and stir and react for 4 h under the condition of heating at 50 °C in a water bath;
[0038] (4) After the reaction, the samples were divided into 2 mL centrifuge tubes, centrifuged at room temperature for 5 min under the condition of 12000 rpm, the supernatant was discarded, and the precipitate w...
Embodiment 2
[0040] Example 2 Synthesis of biological tellurium nanoparticles
[0041] (1) Mix 100g of Moringa stalks (stalks, leaves) with 1000mL of water, use a 2100W induction cooker at 240°C, and stir and heat for 5min;
[0042] (2) The solid-liquid mixture was filtered with 8 layers of gauze, and the crude extract was filtered twice with a water circulating vacuum pump. Finally, 600 mL of filtrate was collected, and the filtrate was divided into 250 mL conical flasks (100 mL / bottle), placed in 2 ℃, stored for 48h;
[0043] (3) In a 50 mL glass beaker, mix 50 mL of the Moringa oleifera straw extract, which was stored at 2°C for 48 hours, with 0.3 g of potassium tellurite, and stir and react for 1 hour under the condition of heating at 25°C in a water bath;
[0044] (4) After the reaction, the samples were divided into 2 mL centrifuge tubes, centrifuged at room temperature for 5 min under the condition of 12000 rpm, the supernatant was discarded, and the precipitate was taken;
[0045...
Embodiment 3
[0046] Example 3 Synthesis of biological tellurium nanoparticles
[0047](1) Mix 100g of Moringa stalks (stalks, leaves) with 1000mL of water, use a 2100W induction cooker at 240°C, and stir and heat for 5min;
[0048] (2) The solid-liquid mixture was filtered with 8 layers of gauze, and the crude extract was filtered twice with a water circulating vacuum pump. Finally, 600 mL of filtrate was collected, and the filtrate was divided into 250 mL conical flasks (100 mL / bottle), placed in 4 ℃, save for 24h;
[0049] (3) In a 50 mL glass beaker, mix 50 mL of the Moringa oleifera straw extract, which was stored at 4°C for 24h, with 0.1g potassium tellurite, and stir and react for 4h under the condition of heating at 28°C in a water bath;
[0050] (4) After the reaction, the samples were divided into 2 mL centrifuge tubes, centrifuged at room temperature for 5 min under the condition of 12000 rpm, the supernatant was discarded, and the precipitate was taken;
[0051] (5) Wash the p...
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