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Moringa oleifera straw mediated synthesized biological tellurium nanoparticles and antibacterial and antiviral application thereof

A technology of synthetic biology and tellurium nanometers, applied in antiviral agents, antibacterial drugs, and resistance to vector-borne diseases, etc., can solve the problems of no reports, etc., and achieve the effect of high antibacterial activity, low source, and high stability

Active Publication Date: 2022-06-03
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, although some plants have been used to synthesize bio-TeNPs (Bio-TeNPs), however, the synthesis of bio-TeNPs from Moringa oleifera has not been reported.

Method used

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  • Moringa oleifera straw mediated synthesized biological tellurium nanoparticles and antibacterial and antiviral application thereof
  • Moringa oleifera straw mediated synthesized biological tellurium nanoparticles and antibacterial and antiviral application thereof
  • Moringa oleifera straw mediated synthesized biological tellurium nanoparticles and antibacterial and antiviral application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Synthesis of biological tellurium nanoparticles

[0035] (1) Mix 100g of Moringa stalks (stalks, leaves) with 1000mL of water, and use a 2100W induction cooker at 240°C to stir and heat for 10min;

[0036] (2) The solid-liquid mixture was filtered with 8 layers of gauze, and the crude extract was filtered twice with a water circulating vacuum pump. Finally, 600 mL of filtrate was collected, and the filtrate was divided into 250 mL conical flasks (100 mL / bottle), placed in 600 mL conical flasks. ℃, save for 16h;

[0037] (3) In a 100 mL glass beaker, mix 50 mL of the Moringa oleifera straw extract, which was stored at 6 °C for 16 h, with 0.2 g of potassium tellurite, and stir and react for 4 h under the condition of heating at 50 °C in a water bath;

[0038] (4) After the reaction, the samples were divided into 2 mL centrifuge tubes, centrifuged at room temperature for 5 min under the condition of 12000 rpm, the supernatant was discarded, and the precipitate w...

Embodiment 2

[0040] Example 2 Synthesis of biological tellurium nanoparticles

[0041] (1) Mix 100g of Moringa stalks (stalks, leaves) with 1000mL of water, use a 2100W induction cooker at 240°C, and stir and heat for 5min;

[0042] (2) The solid-liquid mixture was filtered with 8 layers of gauze, and the crude extract was filtered twice with a water circulating vacuum pump. Finally, 600 mL of filtrate was collected, and the filtrate was divided into 250 mL conical flasks (100 mL / bottle), placed in 2 ℃, stored for 48h;

[0043] (3) In a 50 mL glass beaker, mix 50 mL of the Moringa oleifera straw extract, which was stored at 2°C for 48 hours, with 0.3 g of potassium tellurite, and stir and react for 1 hour under the condition of heating at 25°C in a water bath;

[0044] (4) After the reaction, the samples were divided into 2 mL centrifuge tubes, centrifuged at room temperature for 5 min under the condition of 12000 rpm, the supernatant was discarded, and the precipitate was taken;

[0045...

Embodiment 3

[0046] Example 3 Synthesis of biological tellurium nanoparticles

[0047](1) Mix 100g of Moringa stalks (stalks, leaves) with 1000mL of water, use a 2100W induction cooker at 240°C, and stir and heat for 5min;

[0048] (2) The solid-liquid mixture was filtered with 8 layers of gauze, and the crude extract was filtered twice with a water circulating vacuum pump. Finally, 600 mL of filtrate was collected, and the filtrate was divided into 250 mL conical flasks (100 mL / bottle), placed in 4 ℃, save for 24h;

[0049] (3) In a 50 mL glass beaker, mix 50 mL of the Moringa oleifera straw extract, which was stored at 4°C for 24h, with 0.1g potassium tellurite, and stir and react for 4h under the condition of heating at 28°C in a water bath;

[0050] (4) After the reaction, the samples were divided into 2 mL centrifuge tubes, centrifuged at room temperature for 5 min under the condition of 12000 rpm, the supernatant was discarded, and the precipitate was taken;

[0051] (5) Wash the p...

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Abstract

The invention provides a Moringa oleifera straw mediated synthesized biological tellurium nanoparticle. The Moringa oleifera straw mediated synthesized biological tellurium nanoparticle is prepared by a method comprising the following steps: taking Moringa oleifera straw and water to prepare a solid-liquid mixture; filtering and collecting filtrate; mixing and stirring the filtrate and potassium tellurite to react; centrifuging and taking precipitate; and after washing, re-suspending in deionized water to obtain the product. The invention also provides antibacterial and antiviral application of the biological tellurium nano material. The technical blank of synthesizing the biological tellurium nanoparticles with antibacterial and antiviral functions by using moringa oleifera in the prior art is filled, and the preparation method is low in production cost, simple in synthesis process, safe, efficient, high in product stability, rich in biological function, wide in application range, remarkable in antibacterial effect, good in antiviral effect and environmentally friendly. The method has a wide market prospect.

Description

【Technical field】 [0001] The invention relates to the technical field of biological nanomaterials, in particular to a kind of Moringa straw-mediated synthesis of biological tellurium nanoparticles and its antibacterial and antiviral application. 【Background technique】 [0002] Pathogenic bacteria are a class of bacteria that can cause human and other hosts to treat diseases. Under certain conditions, they can directly or indirectly cause diseases and seriously affect human health. Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus agalactiae and Klebsiella pneumoniae are common pathogenic bacteria in clinical practice. Meningitis, sepsis, etc. Respiratory tract infection is a common disease. Clinically, because the pathogen cannot be identified in time, the mortality rate remains high. More importantly, due to the frequent use of antibiotics, many respiratory tract infection pathogens are resistant to antibiotics to varying degrees. Due to its medicinal proper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K33/04A61K36/185A61P31/04A61P31/16
CPCA61K9/14A61K33/04A61K36/185A61P31/04A61P31/16Y02A50/30
Inventor 夏险何飞吕静胡兵涂俊铭敖波谭峥
Owner HUBEI NORMAL UNIV
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