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Method for extracting dandelion anti-inflammatory component by ultrasonic-assisted deep-eutectic solvent method

A low eutectic solvent, ultrasonic-assisted technology, used in separation methods, solvent extraction, solid solvent extraction and other directions, can solve the problems of inability to store for a long time, the extract is easily contaminated, and the yield of active ingredients is difficult to guarantee. The effect of high rate and optimized process conditions

Pending Publication Date: 2022-06-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the active components of dandelion are extracted by alcohol extraction, water extraction, enzymatic method, etc. The yield of active components is difficult to be guaranteed, and there are obvious disadvantages. For example, the ethanol used in the alcohol extraction method is a flammable and explosive reagent. The extracts extracted by water extraction and enzymatic methods are easily polluted and cannot be stored for a long time

Method used

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  • Method for extracting dandelion anti-inflammatory component by ultrasonic-assisted deep-eutectic solvent method
  • Method for extracting dandelion anti-inflammatory component by ultrasonic-assisted deep-eutectic solvent method
  • Method for extracting dandelion anti-inflammatory component by ultrasonic-assisted deep-eutectic solvent method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Standard curve drawing

[0036] (1) Weigh 0.0208 g of quercetin standard, dilute to a 100 mL volumetric flask with ethanol, and prepare a solution of 208 μg / mL.

[0037] (2) Take 0, 0.5, 1, 1.5, 2, and 2.5 mL of the control solution and place them in a 50 mL quantitative bottle; and dilute to 50 mL with ethanol to prepare 0 μg / mL, 2.08 μg / mL, 4.16 μg / mL, 6.24μg / mL, 8.32μg / mL, 10.4μg / mL solutions;

[0038] (3) Measure the absorbance at the wavelength of 374 nm (at the maximum absorbance value). Take μg / mL as the abscissa and absorbance as the ordinate to establish a standard curve, and obtain the linear regression equation y=0.0656x+0.0036, R 2 = 0.9977, see figure 1 .

Embodiment 2

[0039] Embodiment 2: The optimization method of the process condition of ultrasonic-assisted deep eutectic solvent extraction of anti-inflammatory components of dandelion, the specific steps are as follows

[0040] 2.1 Determination of deep eutectic solvent (DESs) combinations

[0041] The best combination was selected from choline chloride-lactic acid, choline chloride-fructose, choline chloride-ethylene glycol, choline chloride-glycerol, choline chloride-urea, and choline chloride-urea was selected first. : Hydrogen donor = 1:2 molar ratio, put into 50 mL of reagent, place it in an oil bath at 90°C for 1 hour with stirring to ensure that the deep eutectic solvent is clear and transparent, then cut 40 mL into a centrifuge tube, add 10 mL of Ultrapure water and 0.5g of dandelion powder passed through a 40-mesh sieve were prepared into a 50mL solution with a water content of 20%, a solid-liquid ratio of 1%, and a molar ratio of 1:2, and ultrasonicated for 90 minutes in an ultra...

Embodiment 3

[0066] Example 3: Macroporous resin purification of dandelion extract

[0067] The resin column was first plugged with absorbent cotton, and 10 g of the treated dry resin was diluted with 20 mL of water to form a resin aqueous solution and poured into the resin column. After the resin is completely precipitated, add 50 mL of the extract sample solution of the deep eutectic solvent, pass through the resin column at a flow rate of 5 BV / h, do this twice, and then use 200 mL of distilled water to pass through the resin column at a flow rate of 10 BV / h until the flow rate is 10 BV / h. The solution was colorless, thereby removing the deep eutectic solvent remaining in the resin, and finally desorbing the macroporous adsorption resin with about 200 mL of 95% ethanol solution at a flow rate of 10 BV / h until the effluent solution was colorless. The desorbed solution of the 95% ethanol solution was collected and subjected to rotary evaporation to obtain the dandelion solid extract.

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Abstract

The invention belongs to the technical field of natural product extraction, and particularly relates to a method for extracting dandelion anti-inflammatory components through an ultrasonic-assisted deep-eutectic solvent. According to the dandelion anti-inflammatory component extraction method provided by the invention, a novel green eutectic solvent combination is used as an extracting agent for the first time, and the use of a traditional organic solvent is avoided, so that a series of problems of high cost, high risk, high harm, high pollution and the like caused by a traditional organic chemical reagent in a dandelion extraction process are solved.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting anti-inflammatory components of dandelion assisted by an ultrasonic-assisted low eutectic solvent. Background technique [0002] Dandelion is a medicinal and edible homologous plant announced by the National Health Commission, and it is a good dietary element and medical resource. Dandelion was first recorded in "Tang Materia Medica": "Sweet, flat, non-toxic. Housewife's mammary carbuncle." "Compendium of Materia Medica" records that dandelion treats women's mammary carbuncle, detoxifies food, disperses stagnant qi, and resolves heat toxin , Removal of malignant swelling, tuberculosis, Ding swollen. Modern medical research has proved that dandelion mainly contains phenolic acids, flavonoids, polysaccharides, sterols, terpenes, pigments and other components, which have antibacterial, antioxidant, anti-inflammatory, anti-gastr...

Claims

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Application Information

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IPC IPC(8): A61K36/288A61P29/00B01D11/02
CPCA61K36/288A61P29/00B01D11/0288A61K2236/333A61K2236/53A61K2236/51A61K2236/55
Inventor 冯春来芮蒙杰包明
Owner JIANGSU UNIV
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