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Preparation method of bone repair scaffold with drug sustained release and antibacterial effects

A technology of antibacterial effect and bone repair, applied in medical science, prosthesis, etc., can solve the problems of PLLA brittleness, non-union, unfavorable cell adhesion and proliferation, etc., and achieve the effect of accelerating bone repair, promoting proliferation and differentiation

Inactive Publication Date: 2022-07-01
THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the PLLA bone scaffold is implanted in the bone defect, the phenomenon of delayed union or nonunion will occur due to the loss or death of cell activity during the bone repair process, and PLLA is brittle and hydrophobic, which is not conducive to the adhesion of cells on the scaffold. and proliferation, which to some extent limits its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0017] The invention provides a preparation method of a bone repair scaffold with slow drug release and antibacterial effect, which comprises the following steps:

[0018] First, mix MBG powder with PLLA powder, ultrasonically disperse, ball mill, and dry to obtain a composite powder, which is subjected to selective laser sintering to obtain a PLLA / MBG bone scaffold; mesoporous bioactive glass (MBG) is an inorganic material, which After compounding with PLLA polymer, the hydrophobic properties of the polymer can be significantly improved, and the biocompatibility can be improved. As a bone scaffold material, it can provide good mechanical properties and an environment for cell migration and adhesion, and the MBG degradation product is non-toxic and non-toxic. Harmful, can be metabolized by the human body. In the implementation process, the mass ratio of MBG powder to PLLA powder is 1:(5-10), and the particle size of the composite powder is 50-100 microns, so that MBG has enoug...

Embodiment

[0022] According to the mass ratio of 1:10, MBG powder and PLLA powder were mixed, dispersed by ultrasonic, and ball-milled into a composite powder with an average particle size of about 100 microns, which was then dried for use, and then selectively laser sintered into a diameter of about 5 mm and a height of about 5 mm. 10mm cylindrical PLLA / MBG bone scaffold; then, immerse the PLLA / MBG bone scaffold in a 6g / L chitosan hydrogel solution for 1 hour, then put it into an oven to dry at 37°C, take it out, rinse it with ultrapure water, and then rinse it with ultrapure water. Put into 5% NaOH solution for 30min, and then rinsed with ultrapure water for 3 times to obtain PLLA / MBG / CS scaffold; then immerse PLLA / MBG / CS scaffold in 2g / L Slit3 solution for 1 hour at 37℃ , then take out -20°C freeze and dry to obtain a PLLA / MBG / CS scaffold loaded with Slit3, cut out a small section of the PLLA / MBG / CS scaffold to expose the cross section, and detected by electron microscope, the MBG pore...

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PUM

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Abstract

The invention relates to preparation of a bone repair scaffold, in particular to a preparation method of a bone repair scaffold with drug sustained release and antibacterial effects, which comprises the following steps: mixing MBG powder and PLLA powder, performing ultrasonic dispersion, ball milling and drying to obtain composite powder, and performing selective laser sintering to obtain the PLLA / MBG bone scaffold. The PLLA / MBG bone scaffold is immersed in a hydrogel solution prepared from chitosan to prepare a PLLA / MBG / CS scaffold, then the PLLA / MBG / CS scaffold is immersed in a solution prepared from growth factors to obtain the PLLA / MBG / CS scaffold loaded with the growth factors, and a PLLA / MBG scaffold matrix provides mechanical properties and a cell migration and adhesion environment; the chitosan hydrogel attached to the stent provides a matrix for cell growth and has antibacterial property and drug-loading capacity; slit3 or PDGF-BB loaded on the stent can be associated with H-type blood vessels, so that proliferation and differentiation of bone progenitor cells in bone marrow are promoted.

Description

technical field [0001] The invention relates to the preparation of a bone repair bracket, in particular to a preparation method of a bone repair bracket with slow drug release and antibacterial effect. Background technique [0002] Bone graft surgery is required for bone defects caused by trauma, tumors, infections, congenital diseases, and spinal fusion. During the operation, there are many problems, such as long operation time of autologous bone transplantation, limited supply, complications at the donor site, low compatibility between allogeneic bone transplantation and recipient tissue, and prone to rejection. In order to solve these problems, artificial bone substitutes appeared and developed rapidly, and bone transplantation surgery gradually turned to the use of synthetic bone substitutes. Polymer materials are considered to be a therapeutic approach for bone defects due to their favorable biological properties, which provide conditions for cell migration and new tis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/10A61L27/20A61L27/50A61L27/54A61L27/58
CPCA61L27/18A61L27/10A61L27/20A61L27/50A61L27/54A61L27/58A61L2300/602A61L2300/404A61L2300/412A61L2430/02A61L2300/414A61L2300/236C08L67/04C08L5/08
Inventor 陈世杰黄力平曾晋彭毅王栋仝昭宸王卫国李劲松苗惊雷
Owner THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV