Human extracellular matrix metalloproteinase inducible factor magnetic particle chemiluminescence immunoassay quantitative detection kit and detection method thereof
A technology of chemiluminescence immunity and metalloprotease, which is applied in the direction of chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, and measurement devices, can solve problems such as side effects, achieve easy dispersion, solve low sensitivity, and wide detection range Effect
Pending Publication Date: 2022-07-12
XIANGYA HOSPITAL CENT SOUTH UNIV
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Abstract
The invention discloses a magnetic particle chemiluminescence immune quantitative detection kit for a human extracellular matrix metalloproteinase inducible factor and a detection method of the magnetic particle chemiluminescence immune quantitative detection kit for the human extracellular matrix metalloproteinase inducible factor. The kit comprises a magnetic particle suspension coated with streptavidin, a human extracellular matrix metalloproteinase inducible factor CD147 monoclonal antibody labeled by alkaline phosphatase, a human extracellular matrix metalloproteinase inducible factor CD147 monoclonal antibody labeled by biotin, and a magnetic particle suspension coated by the magnetic particle suspension. According to the kit, an antigen of a to-be-detected sample is coupled with a biotin-labeled CD147 monoclonal antibody and an alkaline phosphatase-labeled CD147 monoclonal antibody to form a compound, and after an enzymatic chemiluminescence substrate is added into the compound, a luminescence reaction is formed to detect the luminescence intensity so as to detect the concentration of the human extracellular matrix metalloproteinase-inducing factor in the to-be-detected sample. According to the kit, the defects of narrow linear range, complicated operation and the like of an enzyme linked immunosorbent assay kit are overcome, and the defects of low sensitivity, poor stability, high cost and the like are overcome.
Application Domain
Chemiluminescene/bioluminescenceAgainst vector-borne diseases
Technology Topic
MetalloproteinaseAntigen +12
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PUM
Property | Measurement | Unit |
Particle size | 10.0 ~ 20.0 | nm |
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