Method for increasing yield of antifungal active substance HSAF and application

An active substance, antifungal technology, applied in the field of increasing the production of antifungal active substance HSAF, can solve the problems of bacterial cell death, unsustainable synthesis of HSAF, and complexity.
CN114752618APending Publication Date: 2022-07-15JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Publication Date
2022-07-15

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Abstract

The invention discloses a method for increasing the yield of an antifungal active substance HSAF and application. According to the invention, an RNA chaperone molecular protein Hfq on a lysobacter enzymogenes OH11 strain genome is knocked out by using a homologous recombination method, so that a strain delta Hfq is obtained. The strain delays the autolysis phenomenon, so that the effective fermentation time is prolonged, the yield of the active metabolite HSAF of the strain is increased, and a foundation is laid for industrial development of the HSAF.
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Description

technical field

[0001] The invention belongs to the field of microbial genetic engineering, and in particular relates to a method and application for improving the yield of an antifungal active substance HSAF. Background technique

[0002] Lysobacter (Lysobacter) belongs to the Xanthomonas family, γ-Proteobacteria, and widely exists in various natural environments. The bacteria of this genus can not only secrete a variety of extracellular hydrolases (α-proteolytic enzyme, β-1,3-glucanase, chitinase, etc.), but also synthesize secondary metabolic antibacterial substances, which are to be developed. A rich library of natural active substances.

[0003] HSAF is a substance with broad-spectrum antagonistic activity against pathogenic fungi and oomycetes produced by Lysobacter enzymogenes OH11. The substance has high activity, 0.5μg / mL can significantly inhibit pear rot bacteria, and has very good heat resistance, so it is named heat stable antifungal factor HSAF (Heat Stable A...

Claims

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