Biosensor as well as preparation method and application thereof

A biosensor, tungsten sulfide technology, applied in the direction of material electrochemical variables, can solve the problems of unstable performance, complex operation, low sensitivity, etc., and achieve the effect of fast synthesis, high sensitivity and high sensitivity

Pending Publication Date: 2022-07-15
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problems of complex operation, low sensitivity and unstable performance of existing biosensors, this application provides a biosensor and its preparation method and application

Method used

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  • Biosensor as well as preparation method and application thereof
  • Biosensor as well as preparation method and application thereof
  • Biosensor as well as preparation method and application thereof

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preparation example Construction

[0045] The present application also provides a method for preparing the biosensor, the method includes the following steps: preparing a gold film substrate; preparing a tungsten sulfide crystal, and disposing the tungsten sulfide crystal on the gold film substrate; A modified film is arranged on the tungsten sulfide crystal; DNA to be observed is arranged on the modified film.

[0046] Further, the preparation of the gold film substrate includes cleaning the cover glass and drying it, then placing the cover glass 1 in an electron beam evaporation film deposition system, and plating the chrome layer 2 and the gold layer 3 in sequence. The temperature of chrome plating is 902°C, and the temperature of gold plating is 877°C.

[0047] Specifically, the cover glass 1 was ultrasonically cleaned with acetone before the reaction, then cleaned with ethanol, and finally with N 2 Blow dry.

[0048] The combination of gold and cover glass 1 is not good, while the combination of chromium...

Embodiment

[0058] 1. The coverslips were ultrasonically cleaned with acetone and ethanol, and washed with N 2 Blow dry, placed in E-Beam electron beam evaporation film deposition system, plated with 2nm thick chromium at 902℃, and plated with 48nm gold at 877℃;

[0059] 2. The multi-temperature zone tube furnace is heated to 200°C upstream and 940°C downstream, keeping the argon gas flow at 30sccm, the pressure in the tube at 50Pa, the heating time of the vulcanization reaction is 25min, and the time of the vulcanization reaction is 20min. After cooling to room temperature naturally, a single layer of WS was obtained. 2 two-dimensional crystal;

[0060] 3. Spin-coat PMMA on the long WS 2 After repeating twice, the PMMA film was peeled off, attached to the obtained gold film substrate and dried, and the PMMA was removed with acetone to obtain a single-layer WS. 2 The gold film substrate of the two-dimensional crystal is the carrier film;

[0061] PMMA is generally used for wet transfe...

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Abstract

The invention belongs to the technical field of biological detection, and particularly relates to a biosensor and a preparation method and application thereof. An existing biosensor is complex in operation, low in sensitivity and unstable in performance. The invention provides a biosensor which comprises a carrier film, a modified film and a to-be-observed DNA layer which are sequentially stacked in a first direction, and the first direction is that the carrier film points to the to-be-observed DNA layer; the carrier film comprises a gold film substrate layer and a tungsten sulfide layer which are sequentially stacked in the first direction. By adding a single-layer tungsten sulfide crystal which is a charge sensitive material, on the basis of original pure gold film surface plasmon resonance angle imaging, modulation of different potential amplitude frequencies is added, and the effect of observing lambda DNA more clearly and sensitively is achieved. Operation is simple, sensitivity is high, and performance is stable.

Description

technical field [0001] The present application belongs to the technical field of biological detection, and in particular relates to a biological sensor and its preparation method and application. Background technique [0002] Over the past 20 years, SPR sensors have added many applications to basic biological research, such as medical diagnostics, drug discovery, food safety, precise measurement, and environmental detection. Sensors based on surface plasmon resonance technology have become the leading optical sensing technology due to their sensitivity, simple fabrication, label-free and real-time detection, which play an important role in the detection of biological interactions such as protein binding and DNA hybridization. effect. [0003] In recent years, the biological interaction of DNA-graphene and its biological analysis have attracted much attention. With the development of graphene-like materials, another two-dimensional transition metal dichalcogenide (TMD) mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 薛天宇刘阳王铮向建勇聂安民温福昇牟从普王博翀翟昆柳忠元
Owner YANSHAN UNIV
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