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Acellular allogeneic dermal suspension patch for pelvic organ prolapse treatment

A decellularized and allogeneic technology, applied in prosthetics, medical science, etc., can solve the problems of increased cytotoxicity, mesh erosion, foreign body sensation, etc., and achieve the effect of mild and non-irritating process, enhanced tissue tension, and tissue repair

Pending Publication Date: 2022-07-22
BEIJING JAYYALIFE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, pelvic organ prolapse slings are mainly made of non-absorbable polyester, polypropylene and other materials; these materials are good carriers of bacteria, have poor anti-infection ability, are prone to mesh erosion, and are prone to foreign body sensation and pain when implanted in the body , harden over time, reducing the patient's quality of life
At present, there are also some biologically derived materials used as POP treatment, which are typically prepared from materials such as the small intestinal submucosa. However, if the products made of such materials are not cross-linked, most of them will degrade over time, and the long-term clinical effect cannot be guaranteed; The use of cross-linking agents in the process of material processing is to improve the mechanical properties of the product. However, the cross-linked product will inhibit wound healing during use, and the cytotoxicity of the material will increase in the short term, stimulating local tissue to produce long-term Inflammatory response, some cross-linking agents will release cross-linking agent monomers during long-term tissue repair, causing chronic toxicity to local tissues and the body

Method used

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  • Acellular allogeneic dermal suspension patch for pelvic organ prolapse treatment
  • Acellular allogeneic dermal suspension patch for pelvic organ prolapse treatment
  • Acellular allogeneic dermal suspension patch for pelvic organ prolapse treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The present embodiment provides an acellular allogeneic dermal suspension patch for the treatment of pelvic organ prolapse, and the preparation method is as follows:

[0083] (1) Cut the raw skin (alien skin) (shape and size such as Image 6 shown). The trimmed raw skin was placed in a glycerol saline solution with a glycerol concentration of 50% (v / v) (the solvent used was physiological saline) for immersion treatment at room temperature for 10 hours. The raw material skin after soaking in the glycerol saline solution was taken out, washed with purified water for 5 times, soaked in physiological saline for 2 hours, and washed with purified water for 5 times.

[0084] (2) Trypsin enzymolysis treatment: the raw skin treated in step (1) was soaked and shaken in a mixture of 0.05% trypsin solution and 0.01% EDTA, and the pH value was adjusted to 7.4 with PBS buffer solution, and the shaking speed was 100 rpm, Soak for 2h to stop the enzymatic reaction.

[0085] DNA hydr...

Embodiment 2

[0094] The present embodiment provides an acellular allogeneic dermal suspension patch for the treatment of pelvic organ prolapse, and the preparation method is as follows:

[0095] (1) Cut the raw skin (alien skin) (shape and size such as Image 6 shown).

[0096] The cut pieces were soaked in a glycerol saline solution with a glycerol concentration of 30% (v / v) (the solvent used was physiological saline) at room temperature for 7 h. The raw material skin soaked in the glycerol saline solution was taken out and washed with purified water for 5 times, soaked in physiological saline for 2 hours, and washed with purified water for 5 times.

[0097] (2) Trypsin enzymolysis treatment: the raw skin treated in step (1) was soaked and shaken in a mixture of 0.025% trypsin solution and 0.02% EDTA, and the pH value was adjusted to 7.4 with PBS buffer solution, and the shaking speed was 100 rpm, Soak for 3 hours to stop the enzymatic reaction.

[0098] DNA hydrolase treatment: Take o...

Embodiment 3

[0107] The present embodiment provides an acellular allogeneic dermal suspension patch for the treatment of pelvic organ prolapse, and the preparation method is as follows:

[0108] (1) Cut the raw skin (alien skin) (shape and size such as Figure 8 shown).

[0109] The cut (allogeneic skin) was placed in a glycerol saline solution with a glycerol concentration of 30% (v / v) (the solvent used was physiological saline), and soaked at room temperature for 5 hours. The raw material skin soaked in the glycerol saline solution was taken out and washed with purified water for 5 times, soaked in physiological saline for 4 hours, and washed with purified water for 5 times. Then, it was placed in a mixed solution of 50 mM Tris-HCl and 3 mM EDTA, and was shaken and soaked for 6 hours at room temperature, and the shaking speed was 100 rpm. Washed 5 times with purified water, placed in 10 mM Tris buffer, and immersed in 10 mM Tris buffer at room temperature for 6 h with shaking at 100 rp...

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Abstract

The invention relates to the field of medical biological materials, in particular to a decellularized allogeneic dermis suspension patch for pelvic organ prolapse treatment. The prepared decellularized allogeneic dermis suspension patch can be kept in vivo for a long time without foreign body sensation, is not prone to infection, can reduce the occurrence rate of pain, is suitable in tensile strength and can effectively treat pelvic organ prolapse, tissue damage around the patch cannot be caused after the suspension patch is implanted into an anatomical site, and the suspension patch has the advantages of being safe and reliable. And the patch does not have immunological rejection, and cannot be taken out for the second time due to serious infection caused by the patch.

Description

technical field [0001] The invention relates to the field of medical biological materials, in particular to an acellular allogeneic dermal suspension patch used for the treatment of pelvic organ prolapse and a preparation method thereof. Background technique [0002] Pelvic floor dysfunction (PFD) in women caused by pelvic floor defects or pelvic floor support tissue relaxation is a combination of weak pelvic floor support caused by various causes, which in turn leads to displacement of pelvic organs, which in turn leads to the anatomical location of other pelvic organs and their associated anatomy. Abnormal function. Surgical treatment for pelvic organ prolapse (POP) is continuously used in clinical practice. The traditional surgical method has large trauma, slow postoperative recovery, and high recurrence rate of postoperative pelvic organ prolapse. [0003] At present, the most commonly used clinical treatments for pelvic organ prolapse (POP) are total pelvic floor reco...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/362A61L27/3687A61L27/3691A61L27/50A61L2430/40Y02A50/30
Inventor 孙继煌刘禄斌李文红丛敬
Owner BEIJING JAYYALIFE BIOTECH CO LTD
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