The invention discloses a Chinese medicine probiotic compound preparation for the poultry ovarian maintenance, which comprises the following raw materials in part by weight: 10-20 parts of Radix Astragali, 5-15 parts of Radix Codonopsis, 5-10 parts of acanthopanax, 4-8 parts of Astragali Complanati Semen, 2-5 parts of Cyperus Rotundus L, 1-5 parts of corydalis tuber, 5-15 parts of isatis roots, 15-25 parts of epimeddium, 10-15 parts of motherwort, 4-8 parts of Radix Ranunculi Ternati, 0.001-0.1 part of lactic acid bacteria, 0.001-0.1 part of Bacillus spp, 7-15 parts of selenium yeast, and 2-5 parts of decavitamin. The invention also discloses a preparation method of the Chinese medicine probiotic compound preparation. The Chinese medicine probiotic compound preparation significantly improves the immunity of laying hens, improves the ovarian function, eliminates the genital system damage, promotes the follicular development and ovulation, prolongs the peak period of egg production, and improves the egg quality. The Chinese medicine probiotic compound preparation has the health-care effect on ovaries and low in cost, thus being suitable for the egg-production poultry.