Disease-preventing and growth-promoting trichoderma and application thereof

A trichoderma and microbial technology, applied in the direction of application, microbial-based methods, chemicals for biological control, etc., can solve the hidden dangers of chemical pesticides and pesticide residues, and achieve rapid growth, large spore production, and improved germination Effect

Pending Publication Date: 2022-07-22
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of controlling Gastrodia elata mold disease, Atractylodes root rot and Asarum leaf spot is still based on chemical pesticides, but there are hidden dangers of pesticide residues in chemical pesticides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Disease-preventing and growth-promoting trichoderma and application thereof
  • Disease-preventing and growth-promoting trichoderma and application thereof
  • Disease-preventing and growth-promoting trichoderma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0031] Embodiment 1: The Trichoderma sp. MH, which is used for disease prevention and growth promotion in this embodiment, is preserved in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee, and the deposit number is CGMCC No.40084.

[0032] Strain screening: Using the plate dilution coating method, weigh 10 g of Gastrodia elata rhizosphere soil samples from Tonghua, Jilin in June 2021, put them in 90 ml of sterile water, let stand at 120 rpm for 30 minutes, and take the supernatant for 10 times the concentration Gradient dilution. Take 10 respectively -1 , 10 -2 , 10 -3 100 μL of each concentration of the diluent, and each gradient was spread on 3 PDA medium plates; then placed in an incubator at 28° C. for 3 to 7 days, and the growth of the colonies was observed. Use a sterilized inoculation needle to pick out the suspected Trichoderma strains and place them in a new PDA medium for cultivation in a 28°C incubator; when t...

Embodiment 1

[0037]Pick the Trichoderma (Trichoderma sp.) MH bacterium cake and the medicinal plant pathogenic fungus bacterium cake that diameter is 7mm on the PDA plate, respectively symmetrically inoculate on the PDA medium from the position of the center 2.5cm, contrast does not inoculate Trichoderma sp. ) MH bacteria cake, only inoculated with medicinal plant pathogenic fungus cake, each treatment repeated three times, and cultured at 28°C for 5 days to observe the growth of pathogenic fungi. The medicinal plant pathogenic fungi in this example are Aspergillus flavus, Fusarium oxysporum, and Alternaria tenuissima.

[0038] Under confrontational culture conditions, Trichoderma sp. MH could inhibit the mycelial growth of Aspergillus flavus, Fusarium oxysporum and Alternaria tenuissima to varying degrees. The rates were 85.54%, 84.9% and 76.92% respectively (such as Figures 4 to 6 shown). The results of the confrontation culture experiment showed that Trichoderma sp. MH had inhibitory...

Embodiment 2

[0040] Pick the Trichoderma (Trichoderma sp.) MH bacterium cake that diameter is 7mm on the PDA flat plate and inoculate in the triangular flask that 150mL liquid PDA is housed, in 160rpm, 28 ℃ of shaking shakers, ferment and cultivate 3 days, obtain Trichoderma (Trichoderma sp.) sp.) MH fermentation broth.

[0041] Commercially available oxamidil (30%), mancozeb (80%) and carbendazim (50%) were diluted 800-fold, respectively.

[0042] Take 100 μL Trichoderma sp. MH fermentation broth, 800-fold oxamidil (30%) dilution, 800-fold mancozeb (80%) dilution, and 800-fold carbendazim (50%) dilution, respectively. Coated on a PDA plate, the center of the plate was inoculated with bacteria cakes with a diameter of 7 mm Aspergillus gastrodia, Atractylodes root rot bacteria, Asarum leaf spot bacteria, with sterile water as a control, each group was repeated 3 times, and incubated at a constant temperature at 28°C , and measure the diameter of pathogenic bacteria on the 5th day, calculat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses trichoderma for preventing diseases and promoting growth and application thereof, and relates to a strain and application thereof. The trichoderma viride capable of preventing diseases and promoting growth is trichoderma viride MH and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.40084. The trichoderma viride capable of preventing diseases and promoting growth disclosed by the invention is a trichoderma viride MH. The Trichoderma sp. MH strain disclosed by the invention is rapid in growth and large in sporulation quantity, and has a competitive inhibition effect on a plurality of medicinal plant pathogenic fungi such as gastrodia elata aspergillosis, rhizoma atractylodis root rot and asarum tenuifolia leaf spot, and the Trichoderma sp. MH strain disclosed by the invention has the advantages that the Trichoderma sp. MH strain can be used for preventing and treating various medicinal plant pathogenic fungi such as gastrodia elata aspergillosis, rhizoma atractylodis root rot and asarum tenuifolia; meanwhile, Trichoderma sp. MH can generate biocontrol active substances such as indoleacetic acid, siderophore and protease, and can improve the seed germination of medicinal plants.

Description

technical field [0001] The present invention relates to a strain and its application. Background technique [0002] Tianma, Atractylodes and Asarum, as the main authentic medicinal materials in Northeast China, have high medicinal value and economic value. Gastrodia aspergillosis will interfere with the growth of Armillaria, infect Gastrodia tubers, and cause Gastrodia disease. Atractylodes root rot is a common systemic soil-borne disease in the growth period of Atractylodes. Asarum leaf spot infects the leaves and gradually expands, cutting off the conducting tissue, causing the roots to rot and wither. At present, the main methods to control gastrodia elata mold disease, Atractylodes root rot and Asarum leaf spot are still mainly chemical pesticides, but there are hidden dangers of pesticide residues in chemical pesticides. SUMMARY OF THE INVENTION [0003] In order to prevent and treat aspergillosis of Gastrodia elata, root rot of Atractylodes Rhizoma, and Asarum lea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCA01N63/38Y02A40/10
Inventor 罗守杨田春杰吉丽姚宗沐张加凡张亨飞叶力博
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap