Magnetic micro-motor capable of being used for muscle tissue construction and preparation method of magnetic micro-motor
A muscle tissue and micro-motor technology, applied in biochemical equipment and methods, tissue cell/virus culture devices, stress-stimulated microbial growth methods, etc., can solve uncontrollable, unreported cell encapsulation, and complicated micro-motor processes and other issues, to achieve the effect of safety cost, excellent magnetic response performance and mechanical performance, and broad application prospects
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Embodiment 1
[0032] Example 1 Preparation of Magnetic Micromotor
[0033] A magnetic micromotor that can be used for muscle tissue construction is prepared by the following method ( figure 1 ):
[0034] S1. Design a microfluidic chip: assemble the inner phase capillary, observation capillary and outer phase capillary of silica material coaxially, and connect the inner phase liquid inlet device and the outer phase liquid inlet device to the inner phase capillary tube and the outer phase capillary tube correspondingly. The interfaces are sealed with transparent epoxy resin; the inner diameter of the inner phase capillary is 80 μm, and the inner diameter of the outer phase capillary is 750 μm.
[0035] The inner phase capillary, observation capillary and outer phase capillary made of silica can be replaced with the inner phase capillary, observation capillary and outer phase capillary made of Teflon.
[0036] S2. Preparation of spiral fiber template: use 1wt% sodium alginate solution as the...
Embodiment 2
[0041] Example 2 Magnetic Response Performance Test of Magnetic Micromotor
[0042] A rotating magnetic field is generated by using a permanent magnet or an electromagnet, and the magnetic response characteristics of the magnetic micromotor prepared in Example 1 are further studied by using the rotating magnetic field. like image 3 As shown in (a), in a glass tube, the magnetic micromotors with the same length but different pitches rotate and advance along the capillary in a translational helical motion under the action of a rotating magnetic field. Under the same magnetic field strength, the motion speeds of the magnetic micromotors with different pitches are quite different. image 3 (b) and (c) give the quantitative relationship between the motion speed of the magnetic micromotor and its corresponding pitch, and the results show that the motion speed of the magnetic micromotor increases with the increase of the pitch. Furthermore, compared to image 3 It can be seen fro...
Embodiment 3
[0045] Example 3 Application of Magnetic Micromotors in the Field of Artificial Muscles
[0046] In order to make the magnetic micromotor biocompatible to build large-scale cell structures, based on the method of preparing the magnetic micromotor in Example 1, the biocompatible material GelMA and 0.5-1.5 × 10 were added to the sodium alginate solution. 7 cells / ml muscle cells C2C12, GelMA can provide adhesion sites for muscle cells. In order to facilitate the observation, C2C12 cells were stained with green fluorescent probes on the cell membrane, and the cells were incubated at 37 °C for 15-30 minutes in advance; then the C2C12 cells were mixed with the internal phase solution, and a micromotor embedded in a large number of C2C12 cells was prepared through a microfluidic chip. like Figure 5 As shown in (a), the fluorescence image of embedded C2C12 cells was observed by laser scanning microscope, and it can be seen that C2C12 cells showed good viability inside the micromotor...
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