Unlock instant, AI-driven research and patent intelligence for your innovation.

Bacillus velezensis and application thereof in coconut disease control

A technology of Bacillus Velez and Bacillus, applied in the direction of application, bacteria, fungicides, etc., to achieve the effect of strong selectivity and less resistance to drugs

Pending Publication Date: 2022-08-05
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of Bacillus Velez for the control of coconut diseases at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus velezensis and application thereof in coconut disease control
  • Bacillus velezensis and application thereof in coconut disease control
  • Bacillus velezensis and application thereof in coconut disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and screening of strain X1

[0027] (1) Isolation and purification of strains: obtained by dilution coating method. Select 1 g of healthy coconut garden rhizosphere soil, pass through a 60-mesh sieve, and sieve the soil sample according to 10, 10 2 , 10 3 , 10 4 0.1 mL of each gradient soil dilution solution was applied to the LB medium plate with a pipette, and incubated at 30 °C for 4 d. Single colonies were picked according to the different shapes and colors of colonies growing on the plate, and after repeated streaking and purification, they were transferred to the slant of LB medium and stored at 4°C.

[0028] (2) Preparation of the fermentation broth of the strain to be tested: Inoculate the pure culture of the strain to be tested in a 250 mL conical flask containing 100 mL of LB broth, and culture with constant temperature shaking at 160 r / min and 28°C for 1-2 d.

[0029] (3) Screening of antagonistic strains: using the confrontation cultu...

Embodiment 2

[0033] Example 2 Identification of strain X1

[0034] (1) Observation of the morphology and culture characteristics of the strain

[0035] After inoculating strain X1 on LB solid medium for 2 days at 28°C, the colony is nearly circular, with a smooth and moist surface, irregular edges, milky white or pale yellow (see figure 2 ), opaque, no pigment; under the microscope, the cells are rod-shaped, have spores, and are Gram-positive.

[0036] (2) Physiological and biochemical properties of X1

[0037] The physiological and biochemical identification of bacterial strain X1 mainly refers to the "Common Bacterial System Identification Manual" and "Berger Bacterial Identification Manual", and the identification results are shown in Table 2. The physiological and biochemical characteristics of the X1 strain are in line with the characteristics of Bacillus, which is similar to that of Velez. The type strain of Bacillus velezensis is consistent.

[0038] Table 2 Physiological and bioc...

Embodiment 3

[0044] Example 3 Strain X1 fermentation process

[0045] (1) Strain activation. Picked strains were streaked and inoculated onto LB medium plates, cultured in a constant temperature incubator at 30°C for 24 hours, and when a large number of bacterial cells grew on the plates, they were used to transfer liquid seeds.

[0046] (2) Liquid seed preparation. The activated strains were picked and inoculated into the LB medium, shaken at 30° C., 220 r / min for 28 hours, and a large number of bacterial cells were grown to obtain liquid seeds.

[0047] (3) Liquid fermentation. The liquid seeds prepared in step (2) were inserted into the fermentation medium according to the volume ratio of 5% inoculum, the rotation speed was 180-220r / min, the dissolved oxygen was controlled at 30%, the pH was 7.0-7.5, the temperature was 28°C, and the aeration was cultivated for 72h.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses bacillus velezensis X1 and application thereof in coconut disease prevention and control, and belongs to the technical field of biological prevention and control of plant diseases and the field of microbial resource utilization and development. The bacillus velezensis X1 thallus fermentation liquor and the active metabolite in the fermentation liquor of the bacillus velezensis X1 have a remarkable bacteriostatic effect on the coconut crown rot pathogen (Cetocystis paradoxa) and the leaf spot pathogen (Curvula oryzae), and the bacillus velezensis X1 and the active metabolite in the fermentation liquor of the bacillus velezensis X1 have a remarkable bacteriostatic effect on the coconut crown rot pathogen (Curvula oryzae). The strain is preserved in the China General Microbiological Culture Collection Center on June 7, 2021, is classified and named as Bacillus velezensis, and has a preservation number of CGMCC NO.22539, a preservation number of CGMCC NO.22539, a preservation number of CGMCC NO.22539, a preservation number of CGMCC NO.22539 and a preservation number of CGMCC NO.22539. The bacterial strain has the advantages of safety, high efficiency, environment friendliness, difficulty in generating drug resistance, long disease prevention lasting period and the like, and has huge potential in the aspect of biological prevention and control of coconut diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control and the field of microbial resource utilization and development, and particularly relates to a strain of Bacillus veles and its application in preventing and treating coconut diseases. Background technique [0002] Coconut (scientific name: Cocos nucifera L.) is an evergreen tree of the Palm family. It has been cultivated in my country for more than 2,000 years. Coconut juice and coconut meat contain a lot of protein, fructose, vitamins, potassium, calcium, magnesium, etc. The effect of nourishing qi and expelling wind, coconut shell can be made into various handicrafts, high-grade activated carbon, etc. Coconut is an important landscaping tree species and economic crop in tropical and subtropical regions of my country, mainly distributed in Hainan, Guangdong, Guangxi, Yunnan, Fujian and other places. Up to now, my country's coconut planting area has exceeded 600,000 mu, w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 朱辉牛晓庆刘小玉黄丽云刘立云
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI