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Mildew-proof biological fermentation feed and preparation method thereof

A bio-fermented feed and a technology for fermented feed are applied in the field of mildew-proof bio-fermented feed and its preparation, which can solve the problems of affecting the fermentation and storage of feed, easy introduction of mold, etc., so as to improve fermentation activity, improve nutrient content and fermentation efficiency , Enhance the effect of anti-mildew effect

Inactive Publication Date: 2022-08-09
湖南天府生态农业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned related technologies, the inventor believes that when there is insufficient cleanliness, mold is easily introduced during the preparation and storage of fermented feed, which affects the fermentation and storage of feed, so how to avoid the introduction of mold is an urgent problem to be solved

Method used

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  • Mildew-proof biological fermentation feed and preparation method thereof
  • Mildew-proof biological fermentation feed and preparation method thereof
  • Mildew-proof biological fermentation feed and preparation method thereof

Examples

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Effect test

preparation example 1

[0032] Preparation Example 1: Preparation of Burkholderia gladiolus fermented freeze-dried powder

[0033] S1 activation: The strain of Burkholderia gladiolus preserved in glycerol was streaked and activated on LB solid medium plates, and then the solid medium was cultured at 30° C. for 2 days.

[0034] S2 culture: take the single colony on the plate in step S1 and place it in LB liquid medium, and at a temperature of 28-32° C., shake and culture on a shaking table for 18 hours to obtain a culture solution containing Burkholderia gladiolus.

[0035]S3 Fermentation: adding a fermentation broth to the fermentor, then adding the broth containing Burkholderia gladiolus obtained in step S2 into the fermentor at a volume ratio of 1:100 to the fermentation broth, Fermentation was carried out at a temperature of 30 ° C for 6 days. After the fermentation was completed, the fermentation broth was centrifuged to remove the bacteria, and then the fermentation broth after removal of the ba...

preparation example 2

[0036] Preparation Example 2: Preparation of fermented freeze-dried powder of Streptomyces alba

[0037] Activation of S1: take and preserve the strain of Streptomyces albicans in the modified MM solid medium (the composition of the solid medium is: soybean powder 5.0g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 (0.01 g / L, agar powder 20 g / L) for streak activation, and then the solid medium was incubated at 28°C for 2 days.

[0038] S2 culture: take the single colony on the plate in step S1 and place it in the improved MM liquid medium (the composition of the liquid medium is: soybean powder 5.0g / L, K 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.2g / L, FeSO 4 ·7H 2 O 0.01g / L), and at a temperature of 28°C, shaking cultured on a shaker for 18h to obtain a culture solution containing Streptomyces alba.

[0039] S3 Fermentation: adding a fermentation broth to the fermentor, then adding the broth containing Burkholderia gladiolus obtained in step S2 into the fermentor...

preparation example 3

[0048] Preparation Example 3 Preparation of Lactobacillus jensenii Fermented Seed Liquid

[0049] 1) Single colony purification: Lactobacillus jensenii was activated and stored on a slant; then the activated Lactobacillus jensenii was spread on a petri dish of a newly configured medium, and then cultured at 32° C. for 48 hours to obtain the Lactobacillus jensenii. Lactobacillus spp. single colony seeds.

[0050] 2) Cultivation: inoculate the single colony seeds of Lactobacillus jensenii in step 1) into a newly configured liquid medium for cultivation (cultivation temperature is 32° C., cultivation time is 64h), and a liquid medium containing bacteria is obtained.

[0051] 3) Expanded culture: configure the fermentation broth, then transfer the fermentation broth to the fermentor, according to the volume ratio of the liquid culture medium containing the bacterial classification and the fermented broth to be 1:100, the liquid culture containing the bacterial classification in st...

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Abstract

The invention relates to a mildew-proof biological fermentation feed and a preparation method thereof, and the mildew-proof biological fermentation feed comprises the following raw material components in parts by weight: 100-150 parts of a coarse feed, 2-4 parts of an antagonistic microorganism fermentation product and 10-20 parts of a compound fermentation strain, wherein the antagonistic microorganism fermentation product is composed of freeze-dried powder of a fermentation product of the Bickholderia and freeze-dried powder of a fermentation product of the streptomycete, and the freeze-dried powder of the fermentation product of the Bickholderia and the streptomycete is added into the fermentation raw materials in the application. The activity of aspergillus flavus and aspergillus ochraceus possibly introduced in the fermentation and storage processes of the fermented feed is inhibited, so that the mildew-proof effect of the fermented feed is improved. In order to further enhance the mildewproof effect, two fermentation products of sansevieria trifasciata prain and basil leaves are added into the fermentation raw materials, so that the mildewproof effect of the fermented feed is further improved due to the synergistic effect of the two fermentation products and the microbial fermentation product freeze-dried powder.

Description

technical field [0001] The present application relates to the technical field of microbial fermentation, in particular to a mildew-proof biological fermentation feed and a preparation method thereof. Background technique [0002] Fermented feed refers to the use of microorganisms and compound enzymes as biological feed starter strains to convert macromolecular substances and anti-nutritional factors in feed raw materials under artificially controlled environmental conditions such as oxygen content, temperature, and pH value. decompose to produce palatable and nutritious feed. Fermented feed has the advantages of degrading the anti-nutritional factors in the feed and improving the palatability of the feed. The fermentation process can increase the aroma of the feed and improve the palatability, thereby promoting animal feed intake, increasing animal feed intake, improving animal growth performance, and reducing feed-to-meat ratio. , increase economic benefits; use in animal ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37A23K10/22A23L3/3463A23L3/3571A23L3/3472
CPCA23K10/12A23K10/37A23K10/22A23L3/3463A23L3/3571A23L3/3472Y02P60/87
Inventor 金宇陈金柱饶棒胡颖刘奇
Owner 湖南天府生态农业有限公司
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