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Preparation method of photoelectrochemical sensor for detecting transmembrane glycoprotein CD44 on breast cancer cell surface

A technology for transmembrane glycoproteins and breast cancer cells, which is applied in the synthesis of inorganic nanomaterials and photoelectrochemical biosensing, which can solve the problems of fast electron-hole recombination rate, barriers to batch-to-batch variability of sensor chips, and poor visible light absorption results, etc. question

Pending Publication Date: 2022-08-09
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photoelectrochemical sensors have attracted extensive attention from researchers due to their good stability, low cost, and high detection efficiency, but the batch-to-batch variability of sensor chips has always been a major defect that hinders their wide application; titanium dioxide (TiO 2 ) has received widespread attention in photocatalysis and photoelectrochemical sensing due to its non-toxic, cheap, and stable advantages, but its defects are also obvious. The wider energy band forces it to only absorb short-wavelength ultraviolet light with higher energy. The response of the visible light is poor, and the pure TiO 2 The electron-hole recombination rate is fast, which limits its application in different fields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of TiO 2 -Ag-Ag 2 S heterojunction:

[0026] Set the area to 1*1 cm 2 The conductive side of the FTO glass was placed on the bottom of a 50 mL autoclave, and a mixture containing 8 mL of deionized water, 8 mL of concentrated hydrochloric acid, and 0.2 mL of tetrabutyl titanate was poured into it at 120 o Under C for 8 h, TiO with different densities and thicknesses was obtained 2 Nanoarrays were washed and dried at room temperature in a muffle furnace for 400 o C annealing for 3 h improves the crystallinity;

[0027] For the TiO obtained in the previous step 2The nanoarrays were loaded with metallic silver particles. A silver target was used on a magnetron sputtering coater. The argon pressure was adjusted to 1 Pa, the sputtering power was 40 W, and the sputtering time was 1 min to obtain TiO with different silver particle densities. 2 - Ag composite;

[0028] The TiO obtained in the previous step was 2 -Ag composites were immersed in an aqueous ...

Embodiment 2

[0034] (1) Preparation of TiO 2 -Ag-Ag 2 S heterojunction:

[0035] Set the area to 1*1 cm 2 The conductive side of the FTO glass was spread on the bottom of a 50 mL autoclave, and a mixture containing 25 mL of deionized water, 25 mL of concentrated hydrochloric acid and 1 mL of tetrabutyl titanate was poured. o Under C for 10 h, TiO with different densities and thicknesses was obtained 2 Nanoarrays were washed and dried at room temperature in a muffle furnace at 450 o C annealing for 3 h improves the crystallinity;

[0036] For the TiO obtained in the previous step 2 The nanoarrays were loaded with metallic silver particles. A silver target was used on a magnetron sputtering coater. The argon pressure was adjusted to 2 Pa, the sputtering power was 60 W, and the sputtering time was 2 min to obtain TiO with different silver particle densities. 2 - Ag composite;

[0037] The TiO obtained in the previous step was 2 -Ag composites were immersed in an aqueous solution of so...

Embodiment 3

[0043] (1) Preparation of TiO 2 -Ag-Ag 2 S heterojunction:

[0044] Set the area to 1*1 cm 2 The conductive side of the FTO glass was placed on the bottom of a 50 mL autoclave, and a mixture containing 45 mL of deionized water, 45 mL of concentrated hydrochloric acid, and 1.2 mL of tetrabutyl titanate was poured into it. o Under C for 12 h, TiO with different densities and thicknesses was obtained 2 Nanoarrays were washed and dried at room temperature in a muffle furnace for 500 o C annealing for 3 h improves the crystallinity;

[0045] For the TiO obtained in the previous step 2 The nanoarrays were loaded with metallic silver particles. A silver target was used on a magnetron sputtering coater. The argon pressure was adjusted to 5 Pa, the sputtering power was 80 W, and the sputtering time was 3 min to obtain TiO with different silver particle densities. 2 - Ag composite;

[0046] The TiO obtained in the previous step was 2 -Ag composites were immersed in an aqueous so...

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Abstract

The invention relates to a preparation method of a photoelectrochemical sensor for detecting transmembrane glycoprotein CD44 on the surface of a breast cancer cell. The preparation method comprises the following three steps: loading a TiO2 nano array on the surface of FTO (Fluorine-doped Tin Oxide) conductive glass by a hydrothermal method, uniformly sputtering an Ag nano layer on the surface of the TiO2 nano array by a magnetron sputtering technology, and finally immersing TiO2-Ag into a sodium sulfide solution to realize local vulcanization of Ag to obtain the photoelectric conversion body TiO2-Ag-Ag2S nano composite array. Then, extracting the transmembrane glycoprotein CD44 on the surface of the breast cancer cell MDA-MB-231 by utilizing the subject-object recognition effect of hyaluronic acid and the transmembrane glycoprotein CD44 and a DNA (Deoxyribose Nucleic Acid) strand displacement reaction; finally, the photoelectric material and a target object are assembled on a conductive interface through biological coupling and covalent bonding, compared with other reported methods, the preparation method of the sensor is high in controllability, and particularly a sensing interface with good signal output stability is obtained. The scheme and process mentioned in the method have important reference in the fields of material synthesis, photoelectrochemical sensing, cell detection and the like.

Description

technical field [0001] The invention relates to the field of inorganic nanomaterial synthesis and photoelectrochemical biosensing, in particular to a preparation method of a photoelectrochemical sensor for detecting the transmembrane glycoprotein CD44 on the surface of breast cancer cells. Background technique [0002] Currently, breast cancer, known as the "pink killer", has replaced lung cancer as the world's No. 1 cancer. It has been confirmed that the transmembrane glycoprotein CD44 is closely related to tumor invasion and metastasis, and it has been found that the transmembrane glycoprotein CD44 is ubiquitous in breast tumors. Therefore, if the rapid and sensitive detection of the transmembrane glycoprotein CD44 in body fluids can be realized, it will play an important role in the early diagnosis and treatment of breast cancer. Photoelectrochemical sensors have received extensive attention from researchers due to their good stability, low cost, and high detection effic...

Claims

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Application Information

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IPC IPC(8): G01N27/416
CPCG01N27/416Y02E10/542
Inventor 贾越张诺冷东全魏琴
Owner UNIV OF JINAN
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