Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases

A technology of Helicobacter pylori and psoralen is applied in the directions of anti-infective drugs, anti-bacterial drugs, anti-tumor drugs, etc., and achieves the effects of convenient use, good development and medicinal prospects, and simple preparation process

Inactive Publication Date: 2006-05-10
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, there is no literature report on psoralen and its extracts against Helicobacter pylori infection and treatment of diseases related to Helicobacter pylori infection

Method used

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  • Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases
  • Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases
  • Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Using conventional extraction methods, the psoralen seed powder was extracted with 95% ethanol to obtain the total extract. After the total extract is dissolved in water, it is extracted with petroleum ether and ethyl acetate to obtain petroleum ether, ethyl acetate and water-soluble parts. The extraction rate of petroleum ether part and ethyl acetate part (fat-soluble part) is 9.0-15.0%.

Embodiment 2

[0020] Example 2 Anti-Helicobacter pylori effect in vitro

[0021] Add a certain dose of the total extract of Psoralen and the fat-soluble part of the sample to be tested into a certain amount of nutrient agar medium, mix well, pour into a sterile plate, and inoculate 107CFU / ml in logarithmic growth of Helicobacter spiral Bacillus. After 5 days of microaerobic culture, transfer to a drug-free medium by blind scraping and microaerobic culture at 37°C for 4-5 days. Observe the growth of Helicobacter pylori and measure the minimum bactericidal concentration (Minimum Bactericidal Concentrations, MBCs).

[0022] Sample

[0023] Among them, Hp-Sydney strain 1 (Hp, SS1) is the standard strain; Hp-F44 and Hp-Hudong are clinical strains.

Embodiment 3

[0024] Example 3 In vivo pharmacological test in mice

[0025] Level C 57 50 BL / 6 mice, weighing 20-25g, were gavaged with a certain concentration of Helicobacter pylori (HpSSl) to achieve Hp colonization in the body. Mice colonized with Hp were randomly divided into a blank control group, a positive drug control group, a high-dose group (600mg / kg) for fat-soluble parts of psoralen, a medium-dose group (400mg / kg) and a low-dose group (200mg / kg). Five groups, 10 animals in each group. The positive control group adopts the triple therapy currently used by western medicine: omeprazole 5.2 mg / kg, clarithromycin 65 mg / kg and metronidazole 104 mg / kg are used in combination. The blank control group is the corresponding excipient. The above experimental drugs were prepared into a suspension of a certain concentration with 0.6% sodium carboxymethylcellulose-salad oil (1∶1) and administered via a gastric tube twice a day (0.5 mL per mouse) for continuous administration of 14 day. Thirty day...

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Abstract

The invention relates to the new medical use of Psoralea corylifolia, its extract and its fat-soluble fraction. Psoralea corylifolia, its extract and its fat-soluble fraction have notable effect against Helicobacter pylori and can treat these disease such as gastritis, peptic ulcer and so on caused by infection of Helicobacter pylori. They especially have good therapeutic action on active gastritis, gastric erosion, gastric ulcer, and gastro-duodenal ulcer. They also have therapeutic or preventive action on gastric lymphoma, early carcinoma of stomach, moderate metaplasia and proliferation of intestinal glands.

Description

Technical field [0001] The present invention relates to new uses of traditional Chinese medicines, in particular to the application of psoralen and its extracts in resisting Helicobacter pylori infection and treating diseases related to Helicobacter pylori infection. Background technique [0002] Chronic active gastritis, gastric ulcer and duodenal ulcer, gastric MALT lymphoma and gastric cancer are diseases that seriously endanger human health and life. Medical research has confirmed that the above diseases are all related to Helicobacter pylori (Hp) infection. Statistics show that more than 50% of the world’s population is infected with Helicobacter pylori, especially in developing countries. The infection rate of Helicobacter pylori in adults reaches 60-80%. In 1994, the International Agency for Research on Cancer (IARC) listed Hp as a Class I human carcinogen. At present, the combination of anti-endocrine drugs and antibiotics is used to treat H. pylori infection at home and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/487A61K131/00A61P31/04A61P1/04A61P35/00A61P31/00
CPCA61K36/487A61P1/04A61P31/00A61P31/04A61P35/00
Inventor 岳建民樊成奇董蕾吴艳
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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