Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases
A technology of Helicobacter pylori and psoralen is applied in the directions of anti-infective drugs, anti-bacterial drugs, anti-tumor drugs, etc., and achieves the effects of convenient use, good development and medicinal prospects, and simple preparation process
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Embodiment 1
[0019] Using conventional extraction methods, the psoralen seed powder was extracted with 95% ethanol to obtain the total extract. After the total extract is dissolved in water, it is extracted with petroleum ether and ethyl acetate to obtain petroleum ether, ethyl acetate and water-soluble parts. The extraction rate of petroleum ether part and ethyl acetate part (fat-soluble part) is 9.0-15.0%.
Embodiment 2
[0020] Example 2 Anti-Helicobacter pylori effect in vitro
[0021] Add a certain dose of the total extract of Psoralen and the fat-soluble part of the sample to be tested into a certain amount of nutrient agar medium, mix well, pour into a sterile plate, and inoculate 107CFU / ml in logarithmic growth of Helicobacter spiral Bacillus. After 5 days of microaerobic culture, transfer to a drug-free medium by blind scraping and microaerobic culture at 37°C for 4-5 days. Observe the growth of Helicobacter pylori and measure the minimum bactericidal concentration (Minimum Bactericidal Concentrations, MBCs).
[0022] Sample
[0023] Among them, Hp-Sydney strain 1 (Hp, SS1) is the standard strain; Hp-F44 and Hp-Hudong are clinical strains.
Embodiment 3
[0024] Example 3 In vivo pharmacological test in mice
[0025] Level C 57 50 BL / 6 mice, weighing 20-25g, were gavaged with a certain concentration of Helicobacter pylori (HpSSl) to achieve Hp colonization in the body. Mice colonized with Hp were randomly divided into a blank control group, a positive drug control group, a high-dose group (600mg / kg) for fat-soluble parts of psoralen, a medium-dose group (400mg / kg) and a low-dose group (200mg / kg). Five groups, 10 animals in each group. The positive control group adopts the triple therapy currently used by western medicine: omeprazole 5.2 mg / kg, clarithromycin 65 mg / kg and metronidazole 104 mg / kg are used in combination. The blank control group is the corresponding excipient. The above experimental drugs were prepared into a suspension of a certain concentration with 0.6% sodium carboxymethylcellulose-salad oil (1∶1) and administered via a gastric tube twice a day (0.5 mL per mouse) for continuous administration of 14 day. Thirty day...
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