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Bacillus subtilis preparation

A technology for Bacillus subtilis and preparations, which is applied in the field of preparations containing Bacillus subtilis, can solve the problems of high production cost, secondary pollution, difficulty in curing formulations and the like

Active Publication Date: 2007-06-13
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems that the water quality of the aquaculture water body is deteriorating due to pollution and that the species of micro-ecological water quality improvers for fishery is relatively single or the production cost is high, there is secondary pollution, and the dosage form is difficult to solidify. Bacillus preparation, this improver can effectively improve the water quality of aquaculture water, and is non-toxic and pathogenic to fish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Take pond silt, add 10 times the weight of sterile water to dilute, stir and mix, boil for 8 minutes, cool, and then dilute to 10 times with sterile water -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 Take 0.1ml of liquid with different dilutions, evenly spread it on the LB solid medium (pH 7.0~ 7.2), pick suspicious colonies (colonies with irregular edges), and then inoculate them on another LB solid medium with the above-mentioned composition, identify after pure culture, and obtain Bacillus subtilis B115.

[0063] Bacillus subtilis B115 is inoculated in containing 4ml by weight by tryptone 1%, beef extract 0.25%, NaCl 0.5%, the remainder is water, in the test tube of the liquid culture medium (pH7.0~7.2) that forms, and then The bacteria in the test tube were inoculated into a conical flask containing 250 mL of the above-mentioned liquid medium, cultured on a shaker at 200 rpm, 30°C, for 21 hours to obtain a bacterial liquid, and the concentration of Bacillus su...

Embodiment 2

[0067] What is different from example 1 is that this example fermented medium is made up of bean cake powder 4% by weight, bran 5%, the water of corn flour 5% and surplus; Filler is by weight by zeolite powder: rice bran is 2: 1 Mixed composition, the filling material is mixed with the bacteria-containing paste and then dried at 41°C, and the live bacteria content of the powder preparation is determined to be 9.8×10 9 pcs / g, plus the filling material described in 3 times the weight of the filling material, the bacteria content is 24×10 8 individual / g formulations. Other steps are the same as the example.

Embodiment 3

[0069] This example fermentation medium is made up of bean cake powder 4% by weight, bran 4%, the water of corn flour 3% and surplus, fermentation culture 24 hours; Filling material is by weight: kaolin: corn cob powder is 2: 1 Mixing composition, the live bacteria content of the powdered preparation was determined to be 9.2×10 9 pcs / g, plus the filling material described in the content of 3 times the weight of the filling material, the bacteria content is 22×10 8 individual / g formulations.

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PUM

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Abstract

Bacilli preparation which can advance water quality of cultivation, decrease the harmful and poisonous substance, with no toxicity and pathogenicity to the fish, can increase the benefit of cultivation, contains solid substance of the following culture medium of 1.4-4.0% by the standard of mass, 86-96% the following filler and the other being water. The preparation contains Bacillus subtilis B115 CGMCC NO.1210 1~100*108 / g; the solid substance of culture medium is the mixture of two or three ones from pee powder, bran and corn powder; the filler is of are powder, straw powder the mixture of them, is suitable for water quality modifier.

Description

technical field [0001] The present invention relates to preparations containing Bacillus subtilis, especially water quality improvers. Background technique [0002] In recent years, the aquaculture industry has developed extremely rapidly. A large number of antibiotics and other chemical drugs have been used in the process of large-scale intensification. Although the purpose of increasing production and disease resistance has been achieved, long-term abuse of antibiotics will not only cause drug resistance of pathogenic bacteria, but also aquatic products and The chemical residues of antibiotics in the water environment directly threaten human health. Antibiotic residues have become a technical barrier to the international trade of aquatic products in our country, causing huge losses to the export of aquatic products in our country. In recent years, countries have strictly restricted the use of antibiotics and actively sought their substitutes. [0003] The use of microeco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125
Inventor 沈锦玉尹文林
Owner ZHEJIANG INST OF FRESH WATER FISHERIES