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Method for preparing algin lyase and its application

A technology of alginase and lyase, which is applied in the direction of biochemical equipment and methods, medical preparations containing active ingredients, enzymes, etc., which can solve the obstacles to the development and utilization of alginate oligosaccharides, uneven product molecular weight, and difficult process conditions Control and other issues, to achieve the effect of less miscellaneous protein content, short fermentation time, and good temperature stability

Inactive Publication Date: 2003-10-01
OCEAN UNIV OF CHINA
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Problems solved by technology

At present, acid hydrolysis is commonly used to prepare alginate oligosaccharides from alginate. This method needs to be carried out in a strong acid environment, and the process conditions are not easy to control. The cracked products have uneven molecular weight and poor repeatability, which seriously hinders the development and utilization of alginate oligosaccharides
[0003] In the existing preparation methods of alginate lyase, methods such as ammonium sulfate fractional precipitation and gel chromatography are often used, and finally centrifugal freeze-drying is carried out. The disadvantages of these methods are that the test steps are complicated, the process is long, and the cost is high.

Method used

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Embodiment Construction

[0009] The preparation method of the alginate lyase comprises the steps of firstly preparing a culture medium, then sterilizing, inoculating with Vibrio after cooling, and then vibrating and fermenting on a constant temperature shaker. The medium is composed of sodium alginate, MgSO 4 , FeSO 4 , (NH 4 ) 2 8O 4 、KH 2 PO 4 、K 2 HPO 4 ·3H 2 O, NaCl, their weight ratio is 1:0.03-0.06:0.03-0.06:0.4-1:0.6-1.5:1-3:2-6. The weight in kilograms of each component in this embodiment is 1, 0.04, 0.04, 0.7, 1.0, 2 and 4 successively. The sterilization temperature is 121-125°C. The Vibrio is Vibrio sp.QY101, which can be directly isolated from the surface of kelp, and it is inoculated with the culture medium according to the inoculation amount of 1-5:100 (weight ratio), and then fermented and cultivated at 18-30°C at 220r / min After 12-18 hours, the obtained fermentation broth was centrifuged at room temperature at 13000 g for 10 minutes to remove bacteria. Select an ultrafiltrat...

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Abstract

A process for preparing algin lyase includes such steps as preparing culture medium, sterilizing, cooling, inoculating Vibro sp QY101, shake fermenting culture at constant temp. removing bacteria from fermented liquid, ultrafiltering supernatant and concentrating. Its advantages are short fermenting time, high output and activity and high temp stability. It can be used to prepare alginic oligose.

Description

technical field [0001] The invention relates to a preparation method and application of alginate lyase. Background technique [0002] Alginate is an acidic marine polysaccharide with rich sources and wide applications. It is a linear high molecular weight polymer formed by random combination of d-L-guluronic acid (G) and β-D-mannuronic acid (M). . Fucoidan oligosaccharides can be produced from alginate. Fucoidan oligosaccharides have strong biological activity and are widely used in many fields. In terms of medicine, it has anti-tumor, anti-senile dementia, anti-AIDS, immune function and hematopoietic effects; such as promoting the growth of human epidermal keratinocytes and vascular endothelial cells, promoting the recovery of inflamed tissues, etc.; preventing and treating cardiovascular and cerebrovascular diseases; as A safe and reliable therapeutic agent for dermal ulcers, used for surgery, burns, ulcers, etc., without any side effects. In the field of food industry...

Claims

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Application Information

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IPC IPC(8): A61K38/43C12N1/20C12N9/00C12P19/00
Inventor 于文功宋凯韩峰路新枝宫倩红
Owner OCEAN UNIV OF CHINA
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