Preparation method of CdSe nano-crystalline composite liposome microcapsule bubble used for fluorescence immunity detection

Abstract

The present invention belongs to the field of nano material and biological technology. The composite nano CdSe liposome microvesicle for fluorescent immunoassay is prepared through three steps of: preparing nano oily CdSe semiconductor crystal, synthesizing composite nano oily crystal microvesicle, and electrostatically connecting microvesicle with biological protein via altering pH value to regulate surface potential. The present invention is mainly used in test paper strip for immunochromatographic detection of hepatitis B virus, pesticide residue in vegetable and drug detection. The lipophilic surface nano CdSe crystal is phase transferred to constitute biocompatibility.

Description

Technical field [0001] The invention belongs to the field of nano materials and biotechnology, and relates to nano biotechnology, in particular to a method for preparing CdSe nanocrystal composite liposome microvesicles for fluorescence immunoassay. Background technique [0002] For biological and biomedical detection, quantum dots are a kind of fluorescent probes with potential applications. Traditional fluorescent probes generally use organic dyes. Organic dyes are used as biological detection probes, which have many drawbacks. It is mainly manifested in that the half-width of the fluorescence spectrum emitted by organic dyes is about 3-5 times the half-width of the fluorescence spectrum of quantum dots. This will bring overlap of the spectrum and affect the sensitivity of detection. At the same time, the excitation wavelength of the organic dyes It is narrower than quantum dots, which limits the range of excitation light sources, that is, a dye must be excited with a specific ...

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Problems solved by technology

However, the disadvantage of these two methods is that the quantum dots and protein coupling process will bring aggregation and sedimentation of quantum dots and proteins

In addition, for thiol-modified quantum dots, the thiol group is excessive, so that the protein will bind to the free thioglycolic acid in the solution during the interaction of the protein with thioglycolic acid, which will affect the detection sensitivity.

Then in 2000, Nie coated the quantum dots with polystyrene beads, but the polystyrene beads would swell in the solution, resulting in leakage of the quantum dots, which also limited the application of nanocrystals.

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