Preparing method of integrated capillary electrophoresis electrochemical luminescence detecting chip

A technology of capillary electrophoresis and luminescence detection, which is applied in the direction of optomechanical equipment, electric excitation analysis, photoplate making process of patterned surface, etc., can solve the problems of cumbersome operation process, time-consuming, high equipment requirements, etc., and achieve convenient use, simple production, Inexpensive effect

Inactive Publication Date: 2004-11-10
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the manufacturing process of such chips and electrodes is complicated, the requirements for equipment are high, the system stability is poor, and the indirect detection limits the practical application of this system.
Fang has also reported short-distance quartz capillary electrophoresis electrochemiluminescence detection (Anal.Chim.Acta2002, 456, 167-175), but this system cannot be regarded as a true capillary electrophoresis chip, and its working electrode is an additional platinum electrode. The optical signal is transmitted to the photomultiplier tube through an additional optical fiber for detection. The operation process is cumbersome and time-consuming, and the entire system is complicated and not integrated.

Method used

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  • Preparing method of integrated capillary electrophoresis electrochemical luminescence detecting chip
  • Preparing method of integrated capillary electrophoresis electrochemical luminescence detecting chip
  • Preparing method of integrated capillary electrophoresis electrochemical luminescence detecting chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: The mask required for photolithography is a transparent film with a channel pattern output by a high-definition 3000dpi laser printer. Covered on the chrome plate glass coated with photoresist, exposed by ultraviolet light, then developed with developer and removed the chrome layer, cleaned, placed in 1.7%: 1.3% HF / NH 4 In the F glass etching solution, the exposed glass part is etched for 15 minutes, and after the etching is completed, the excess photoresist and the chromium layer are removed with 2% sodium hydroxide solution. Wash the glass template and let it dry. The PDMS monomer and curing agent were mixed at a ratio of 10:1, degassed by a vacuum pump, then poured on a glass template, and heated in an oven at 60°C for 2 hours to cure. After being fully cured, the PDMS layer is peeled off from the glass template, and then the PDMS is cut into the desired chip shape with a blade. The liquid pool of the chip is made by punching holes in the PDMS layer w...

Embodiment 2

[0022] Embodiment 2: The mask required for photolithography is a transparent film with a channel pattern output by a high-definition 3000dpi laser printer. Covered on the chrome plate glass coated with photoresist, exposed by ultraviolet light, then developed with developer and removed the chrome layer, cleaned, placed in 1.7%: 1.3% HF / NH 4In the F glass etching solution, the exposed glass part was etched for 20 minutes, and after the etching was completed, the excess photoresist and the chromium layer were removed with 2% sodium hydroxide solution. Wash the glass template and let it dry. The PDMS monomer and curing agent were mixed at a ratio of 10:1, degassed by a vacuum pump, then poured on a glass template, and heated in an oven at 70°C for 1.5 hours to cure. After being fully cured, the PDMS layer is peeled off from the glass template, and then the PDMS is cut into the desired chip shape with a blade. The liquid pool of the chip is made by punching holes in the PDMS lay...

Embodiment 3

[0026] Embodiment 3: The mask required for photolithography is a transparent film with a channel pattern output by a high-definition 3000dpi laser printer. Covered on the chrome plate glass coated with photoresist, exposed by ultraviolet light, then developed with developer and removed the chrome layer, cleaned, placed in 1.7%: 1.3% HF / NH 4 In the F glass etching solution, the exposed glass part was etched for 30 minutes, and after the etching was completed, the excess photoresist and the chromium layer were removed with 2% sodium hydroxide solution. Wash the glass template and let it dry. The PDMS monomer and curing agent were mixed at a ratio of 10:1, degassed by a vacuum pump, then poured on a glass template, and heated in an oven at 80°C for 1 hour to cure. After being fully cured, the PDMS layer is peeled off from the glass template, and then the PDMS is cut into the desired chip shape with a blade. The liquid pool of the chip is made by punching holes in the PDMS layer...

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Abstract

The invention is a preparation of integrated capillary electrophoresis electrochemical lighting detecting chips, adopting polydimethyl siloxane (PDMS) / glass as chip material, the conductive glass with indium tin oxide (ITO) coating as working electrode, where the working electrode is made by photoetching method and acts as chip basal plate, and reversibly binding the basal plate together with the PDMS layer to compose the whole chip. The making course is simple, easy to operate and reduces chip cost. The detecting chip can be widely applied to detect and analyze amine-containing drug molecules, multiple amino acids, alkylamine, oxalic acid and oxalate, and NADH.

Description

technical field [0001] The invention belongs to a preparation method of an integrated capillary electrophoresis electrochemiluminescence detection chip. technical background [0002] In recent years, more and more people pay attention to the micro-total analysis system. It is an analysis system composed of functional units such as microchannels, microreaction pools, and microreactors fabricated on the chip by using micromachining technology. In this system, chemical experiments such as concentration, separation, reaction, and detection of samples can be performed. The entire chemical analysis process from sample injection to detection is integrated into a micro system for automatic and rapid analysis. As an important part of the microanalysis system, chip capillary electrophoresis has attracted people's attention for its outstanding advantages such as fast analysis speed, high information content, low operating cost, and small sample consumption. However, the development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/66G01N27/447G03F7/00
Inventor 汪尔康杨秀荣邱海波严吉林
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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